FastQCFastQC Report
Tue 21 Dec 2021
000000000-JVJ4T_l01_n01_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JVJ4T_l01_n01_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2886269
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG767842.6603202958559993TruSeq Adapter, Index 21 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA92890.3218341741535526TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC51830.17957439171470158TruSeq Adapter, Index 21 (98% over 50bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT51250.1775648770090383TruSeq Adapter, Index 21 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGACCTCGTATG31690.10979572590080827TruSeq Adapter, Index 21 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)