FastQCFastQC Report
Tue 21 Dec 2021
000000000-JVJ4T_l01_n01_28B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JVJ4T_l01_n01_28B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4341930
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG1790024.12263670763923TruSeq Adapter, Index 20 (97% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG146290.3369239025041859TruSeq Adapter, Index 20 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA115590.266218018254555TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC102380.23579375991782456TruSeq Adapter, Index 20 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCACCTCGTATG66550.15327285331638235TruSeq Adapter, Index 20 (97% over 39bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCC64640.14887388787935318TruSeq Adapter, Index 20 (100% over 39bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC61940.1426554550626104TruSeq Adapter, Index 20 (97% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)