FastQCFastQC Report
Wed 12 Jan 2022
000000000-JNBDR_l01_n01_HW6_22_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNBDR_l01_n01_HW6_22_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167577
Sequences flagged as poor quality0
Sequence length301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGAAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG104636.243696927382636No Hit
ACTCGAAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG101136.034837716393062No Hit
TGCTGTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG89255.3259098802341605No Hit
TGCTGTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG82394.916545826694594No Hit
CTGGTTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG55133.289830943387219No Hit
GACTACAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG52233.1167761685672852No Hit
CTGGTTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG49102.929996359882323No Hit
GACTACAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG38122.274775177977885No Hit
ACTCGAAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG22221.3259576194823872No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA20241.2078029801225705No Hit
ACTCGAAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG15900.9488175584954976No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA14820.884369573390143No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT11870.7083310955560728No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT9840.5871927531821193No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA9060.5406469861615854No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA9000.5370665425446213No Hit
CTGGTTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG8830.5269219522965561No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA7440.4439750085035536No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA7170.4278630122272149No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT6630.3956390196745377No Hit
CTGGTTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG6530.3896716136462641No Hit
GACTACAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG6510.3884781324406094No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT6480.3866879106321273No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGAT6230.3717693955614434No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA5220.31149859467588037No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA4970.2965800796051964No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4930.29419311719388697No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGAT4790.285838748754304No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA4680.27927460212320304No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA4570.2727104554921021No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4390.26196912464120975No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT4190.2500343125846626No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA4140.2470506095705258No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA4120.24585712836487106No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA4000.2386962411309428No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT3890.23213209449984185No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA3540.21124617340088436No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT3170.18916677109627214No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA3110.185586327479308No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA3040.18140914325951654No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT2960.17663521843689767No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2880.17186129361427882No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2720.1623134439690411No Hit
GACTACAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG2610.15574929733794018No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2490.1485884101040119No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACTATGGAA2390.14262100407573833No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA2330.13904056045877417No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2300.1372503386502921No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT2160.12889597021070912No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT2130.12710574840222702No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT2060.12292856418243553No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACTATGGAA1980.11815463935981668No Hit
TGCTGTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG1920.11457419574285253No Hit
TGCTGTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG1910.11397745514002519No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA1740.1038328648919601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)