FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296789
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC780526.018866600503243No Hit
AAGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC720775.558113154877161No Hit
TCGTAGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC653465.0390618674279315No Hit
CCTCTTACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC514373.966489536848323No Hit
AACGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC45270.34909302901242995No Hit
TCCTAGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC35610.27460134223840577No Hit
GCCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC35160.27113123260607547No Hit
ACGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC32030.24699469227453347No Hit
TCGTCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC25160.1940176852209573No Hit
TCGTACACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC22540.17381393580605636No Hit
GTCAGCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC20750.1600106108241202No Hit
CCCCTTACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC20560.15854545342380294No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT19840.15299327801207444No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT19720.15206791544345302No Hit
TCGTCGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC18990.1464386264843394No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT17980.13865015819844248No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT17630.1359511840399633No Hit
TCGTATACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC17390.1341004589027205No Hit
ACGTCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC16990.13101591700731577No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT16630.1282398293014515No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT16320.12584930933251284No Hit
TCCTGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC16290.1256179686903575No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT15780.12168517777371647No Hit
TCCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC14590.11250866563488741No Hit
AACACCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC14500.11181464370842134No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT14300.11027237276071897No Hit
TTCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC13800.10641669539146308No Hit
AAGTCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC13570.10464308380160536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)