FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n02_HW6_90_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n02_HW6_90_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154912
Sequences flagged as poor quality0
Sequence length301
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC3235220.88411485230324No Hit
AAGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC2618216.90120842801074No Hit
GTCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC2000212.911846725883084No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT15490.9999225366659781No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT15110.9753924808923776No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT10100.6519830613509606No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT8870.5725831439785168No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT7030.45380603181160917No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT6670.4305670316050403No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA5460.3524581697996282No Hit
TCGTAGACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT4150.26789403015905805No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT4120.26595744680851063No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT3900.251755835571163No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA3480.22464366866349927No Hit
GTCAGGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTT3120.2014046684569304No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT2410.1555721958273084No Hit
TCGTAGACGTCATCCCCACCTTCCTCACAGTTTGCACTGGCAGTCTTGTT2310.14911691799215038No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT2230.14395269572402394No Hit
AACGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC1940.12523239000206568No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTACCTT1860.12006816773393927No Hit
ACGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC1630.1052210287130758No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA1560.1007023342284652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)