FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n02_HW6_86_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n02_HW6_86_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168224
Sequences flagged as poor quality0
Sequence length301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC4014423.863420201635915No Hit
AAGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC3566121.198521019592924No Hit
GTCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC2376014.124025109377971No Hit
CCTCTTACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC33782.0080369031767167No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT13700.8143903366939318No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT12470.741273540041849No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT11480.6824234354194407No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT8280.49220087502377785No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT8050.47852862849533956No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT6220.3697451017690698No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT5860.34834506372455776No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA4940.29365607761080464No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT4720.2805782765836028No Hit
TCGTAGACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT4500.267500475556401No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT3950.2348059729883964No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTACCTT2840.16882252235115083No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT2570.15277249381776678No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA2240.13315579227696406No Hit
AAGGCCACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTT2180.12958911926954536No Hit
AACGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC2130.12661689176336313No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA2000.11888910024728933No Hit
ACGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC1990.11829465474605287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)