FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n02_HW6_66_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n02_HW6_66_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175691
Sequences flagged as poor quality0
Sequence length301
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC4877127.759532360792527No Hit
AAGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC3272518.626452123330164No Hit
GTCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC2776215.801606229118168No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT15440.8788156479273269No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT14720.837834607350405No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT11560.6579733737072474No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT9270.5276308974278705No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT6730.38305889317039576No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT5870.3341093169257389No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT5770.3284175057344998No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA5200.29597418194443653No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA4860.2766220238942234No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT4340.24702460569977971No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT4160.23677934555554922No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTACCTT3710.21116619519497298No Hit
TCGTAGACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT3700.21059701407584908No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT2590.14741790985309436No Hit
AACGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC2320.13205001963674862No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA2310.1314808385176247No Hit
ACGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC2170.12351230284988986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)