FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n02_HW6_32_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n02_HW6_32_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224556
Sequences flagged as poor quality0
Sequence length301
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC8373337.288248810987014No Hit
CCTCTTACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC2784512.400024938100072No Hit
TCGTAGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC186168.290136981421115No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT21000.9351787527387377No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT17300.770409162970484No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT16040.7142984378061598No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT14820.6599690055041949No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT12320.5486382016067262No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT12040.5361691515702096No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA10980.4889648907176829No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA8240.3669463296460571No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT7080.3152888366376316No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA7020.3126168973440923No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT6760.30103849373875563No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT6040.2689752222162846No Hit
TCGTAGACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT5790.2578421418265377No Hit
CCTCTTACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT5020.22355225422611733No Hit
GCCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC4650.20707529524929194No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTACCTT3930.17501202372682093No Hit
GTCAGGACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT2860.12736243965870428No Hit
GTCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGCGC2500.11133080389746877No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTACCTT2270.10108836993890165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)