FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n02_HW6_25_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n02_HW6_25_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248270
Sequences flagged as poor quality0
Sequence length301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTTACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC5799523.359648769484835No Hit
TCGTAGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC5454821.97124098763443No Hit
AAGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC3631114.62560921577315No Hit
GTCAGGACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC168206.77488218471825No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT18580.7483787811656664No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT16810.6770854311837918No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT15350.6182784871309461No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT15320.6170701252668466No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT12580.5067064083457526No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT10440.42050992870665005No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCGGTTCCTTT8990.3621057719418375No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT8800.35445281346920693No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATT7660.3085350626334233No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT6530.26302009908567286No Hit
CCTCTTACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA6410.25818665162927457No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA4660.18769887622346637No Hit
CCTCTTACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT4580.18447657791920086No Hit
CCTCTTACGCCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC3730.15023965843637974No Hit
TCGTAGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTCCTA3730.15023965843637974No Hit
AAGGCCACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT3310.13332259233898577No Hit
ACGGCCACGTCATCCCCACCTTCCAGTGGGGTATGTGTCAGTTCCTGAGC3080.12405848471422243No Hit
GTCAGGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT2990.1204333991219237No Hit
AAGGCCACGTCATCCCCACCTTCCTCACAGTTTGCACTGGCAGTCTTGTT2820.1135860152253595No Hit
TCGTAGACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCT2790.11237765336125992No Hit
CCTCTTACGTCATCCCCACCTTCCAGTGTGGTATGTGTCAGTTCCTGAGC2560.10311354573649656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)