FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n01_HW6_90_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n01_HW6_90_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154912
Sequences flagged as poor quality0
Sequence length301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG117097.558484817186533No Hit
TGCTGTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG110427.1279177855814915No Hit
ACTCGAAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG102606.623115058872134No Hit
ACTCGAAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG96316.217078083040694No Hit
CTGGTTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG91325.894959719066309No Hit
CTGGTTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG79275.117098739929767No Hit
GACTACAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG74044.779487709151002No Hit
GACTACAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG56763.664015699235695No Hit
CTGGTTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG21101.3620636232183432No Hit
CTGGTTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG15110.9753924808923776No Hit
ACTCGAAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG10150.6552107002685396No Hit
GACTACAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG9810.6332627556290024No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA7160.4621978929973146No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA6940.447996281759967No Hit
ACTCGAAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG6650.4292759760380087No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA5080.3279281140260277No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT5030.3247004751084487No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA4940.31889072505680643No Hit
GACTACAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG4220.27241272464366867No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA4000.25821111340632097No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA3930.2536924189217104No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA3820.24659161330303656No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA3630.2343265854162363No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT3620.2336810576327205No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT3620.2336810576327205No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA3460.22335261309646767No Hit
TGCTGTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG3220.2078599462920884No Hit
TGCTGTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG3160.2039867795909936No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA3030.19559491840528817No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT2920.18849411278661435No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2790.1801022516009089No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT2180.14072505680644495No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2050.13233319562073953No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA1910.12329580665151828No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA1810.11684052881636026No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT1650.1065120842801074No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT1650.1065120842801074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)