FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n01_HW6_80_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n01_HW6_80_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213588
Sequences flagged as poor quality0
Sequence length301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG169277.925070696855628No Hit
TGCTGTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG147886.923609940633368No Hit
TGCTGTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG125825.890780380920276No Hit
CTGGTTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG117305.491881566380133No Hit
GACTACAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG114865.377642938741877No Hit
ACTCGAAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG110495.173043429406146No Hit
ACTCGAAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG104214.879019420566698No Hit
CTGGTTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG102274.788190347772347No Hit
CTGGTTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG21290.9967788452534787No Hit
ACTCGAAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG19790.9265501807217633No Hit
GACTACAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG18520.8670899114182444No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA14630.6849635747326629No Hit
ACTCGAAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG14440.676067943891979No Hit
CTGGTTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG14030.6568721089199768No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA11870.5557428319943069No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA9980.4672547146843456No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA9100.4260538981590726No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA8750.4096672097683391No Hit
GACTACAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG7720.36144352678989455No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA7710.36097533569301643No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT7610.3562934247242354No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA6840.3202427102646216No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA6110.2860647601925202No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT6000.28091465812686106No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT5330.24954585463602824No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT5190.2429911792797348No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4900.22941363747026985No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4490.21021780249826771No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA4350.20366312714197427No Hit
TGCTGTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG4310.20179036275446183No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA4080.19102196752626552No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA4010.18774462984811882No Hit
TGCTGTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG3920.18353090997621588No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA3670.17182613255426335No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT3650.17088975036050716No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA3410.1596531640354327No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT3400.1591849729385546No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA3350.1568440174541641No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2810.1315616982227466No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2740.12828436054459988No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGAT2700.12641159615708747No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT2560.11985692080079405No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA2560.11985692080079405No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT2520.11798415641328165No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2520.11798415641328165No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2470.11564320092889115No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2350.11002490776635392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)