FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n01_HW6_32_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n01_HW6_32_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224556
Sequences flagged as poor quality0
Sequence length301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG193818.63080924134737No Hit
TGCTGTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG187698.358271433406367No Hit
TGCTGTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG174657.7775699602771695No Hit
CTGGTTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG142936.365004720426085No Hit
GACTACAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG134345.982472078234383No Hit
CTGGTTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG124245.532695630488608No Hit
ACTCGAAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG115225.131014090026541No Hit
ACTCGAAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG111904.983166782450703No Hit
CTGGTTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG32341.4401752792176563No Hit
GACTACAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG24611.0959404335666827No Hit
ACTCGAAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG23601.0509627887921054No Hit
CTGGTTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG20850.9284989045048897No Hit
ACTCGAAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG17290.7699638397548941No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA11520.5130123443595361No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA10660.47471454781880684No Hit
GACTACAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG10120.45066709417695366No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA8240.3669463296460571No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA7490.33354708847681647No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA7270.3237499777338392No Hit
TGCTGTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG6950.30949963483496323No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA6690.29792123122962644No Hit
TGCTGTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG6610.2943586455049075No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA6470.2881241204866492No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT5270.2346853346158642No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA5210.23201339532232493No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT5180.23067742567555533No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA5170.23023210245996542No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4840.21553643634549957No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4680.20841126489606157No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4420.1968328612907248No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4340.19327027556600582No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA4320.19237962913482606No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA4010.17857460945153994No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT3200.14250342898876003No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT2790.12424517714957517No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2770.1233545307183954No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT2760.12290920750280554No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2710.12068259142485616No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA2660.11845597534690679No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2470.10999483425069917No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2330.1037603092324409No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA2300.10242433958567128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)