FastQCFastQC Report
Fri 11 Feb 2022
000000000-JNB4T_l01_n01_HW6_25_v2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JNB4T_l01_n01_HW6_25_v2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248270
Sequences flagged as poor quality0
Sequence length301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG3495514.079429653200146No Hit
GACTACAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG244439.845329681395256No Hit
TGCTGTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG188107.576428887904298No Hit
ACTCGAAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG171966.92633020501873No Hit
ACTCGAAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG162456.543279494099166No Hit
TGCTGTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG158916.400692794135416No Hit
CTGGTTAACAGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG129025.196761590204213No Hit
CTGGTTAACCGGATTAGATACCCGGGTGACCCCCGTTCGCCTTCGATCGG110154.436701977685584No Hit
GACTACAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG54822.208079912997946No Hit
ACTCGAAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG28561.1503604946227897No Hit
GACTACAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG23800.9586337455189914No Hit
CTGGTTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG20560.8281306641962379No Hit
ACTCGAAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG20300.817658194707375No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT12910.5199983888508478No Hit
CTGGTTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG12270.49422000241672376No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT11310.45555242276553753No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA11080.44628831514077416No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA9440.38023119990333104No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA8240.3318967253393483No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA7050.2839650380633987No Hit
TGCTGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA6510.2622145245096065No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA6170.24851975671647802No Hit
TGCTGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA5330.2146856245216901No Hit
TGCTGTAACCGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG5300.21347726265759054No Hit
TGCTGTAACAGGATTAGATACCCTGGTGACCCCCGTTCGCCTTCGATCGG5290.21307447536955734No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT5140.20703266604905948No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4740.1909211745277319No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA4570.1840737906311677No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA4510.18165706690296854No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT4000.16111491521327587No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA3720.14983687114834657No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT3460.13936440165948363No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT3370.13573931606718492No Hit
ACTCGAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA3180.1280863575945543No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA3130.12607242115438838No Hit
ACTCGAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA3080.12405848471422243No Hit
CTGGTTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2950.11882224996979096No Hit
CTGGTTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT2850.11479437708945905No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCG2630.10593305675272889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)