FastQCFastQC Report
Fri 29 Jan 2021
000000000-JHLT4_l01_n02_HK1073_99_redo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JHLT4_l01_n02_HK1073_99_redo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299353
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC25350.8468263221013319No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG16740.5592060209852582No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT9530.3183532485059445No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG7570.25287870841448057No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA7010.2341716969597766No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT6570.2194733308167949No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC6550.21880522326484117No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA6520.2178030619369106No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT6380.2131263090732346No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT5890.19675767405036862No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA5820.19441929761853063No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT5740.19174686741071578No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA5630.18807227587497036No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT5510.1840636305632481No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT5350.17871877014761836No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC5330.17805066259566465No Hit
CTCATATGCAATCCTTGTTCTTCGTCCATTATCGCCTCTCCAGAAGTTCC5270.1760463399398035No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT5260.17571228616382664No Hit
ACTATATGCTTTCCTTCACGAGTGGCAGTTCTGATGTCCAGACCAAGAGT5140.17170364085210438No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA5110.1707014795241738No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA5030.16802904931635895No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT5010.16736094176440522No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA5000.16702688798842838No Hit
GCGAAAGCAGGCACTGAAGCGATAGTCATTTTAAGTGCCTCGTCAGATTC4940.16502256533256723No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA4640.15500095205326153No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC4490.1499901454136087No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG4420.14765176898177068No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT4320.14431123122200212No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT4310.14397717744602528No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA4290.14330906989407155No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG4250.14197285479016414No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC4230.1413047472382104No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG4230.1413047472382104No Hit
CTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA4130.13796420947844182No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT4120.13763015570246498No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA4100.13696204815051127No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC4020.1342896179426964No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAACATCATGG4000.13362151039074271No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT3980.132953402838789No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA3970.13261934906281214No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA3950.1319512415108584No Hit
ATCCAGAACAGTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGA3870.12927881130304358No Hit
ACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAACAGGTA3850.12861070375108985No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG3810.12727448864718244No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT3800.12694043487120554No Hit
GTCATATTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA3660.12226368200752957No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3620.12092746690362213No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC3570.11925719802373785No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT3570.11925719802373785No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT3560.11892314424776101No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT3550.11858909047178415No Hit
CTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAG3520.11758692914385357No Hit
GTGCAGACCGTGCCAGAAAGATGAGATCTTCAATTTCAGCATTCCCAGGA3510.11725287536787671No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT3500.11691882159189987No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC3490.11658476781592302No Hit
GGCTAGTACCATTCTGTTCTCATGCCTGATTAGTGGGTTGGTGGTAGTCG3450.11524855271201559No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT3390.11324423005615444No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG3360.11224206872822387No Hit
GAGCAAAAGCAGGCACTGAAGCGATAGTCATTTTAAGTGCCTCGTCAGAT3340.11157396117627016No Hit
GAACTAGGGTGAGTCCCAATTGTCCTCATAGCCTGCACCATTTGCCTAGC3290.10990369229638586No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT3250.10856747719247845No Hit
CAACATGGACAGGGCAGTTAAACTATACAAGAAGCTGAAGAGGGAAATGA3240.10823342341650158No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA3220.10756531586454787No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC3190.1065631545366173No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT3170.1058950469846636No Hit
GTACTAGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAG3170.1058950469846636No Hit
ATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTC3170.1058950469846636No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC3070.10255450922489504No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)