FastQCFastQC Report
Fri 29 Jan 2021
000000000-JHLT4_l01_n02_1805_d06_redo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JHLT4_l01_n02_1805_d06_redo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254714
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG5190.20375794027811586No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT4460.17509834559545215No Hit
GAGCAAAAGCAGAACGGATAAGCAAGAGTAGGCTGGATAAGACGCGCCAG4050.15900186091066842No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGCATTTTTTTCTGCGCCAATGCA3780.1484017368499572No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA3400.13348304372747474No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACATCCGGC3260.12798668310340225No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGT3150.123668114041631No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCT3150.123668114041631No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG2810.11031980966888352No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGA2770.10874942091914853No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG2760.10835682373171479No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA2720.10678643498197979No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC2670.10482344904481104No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA2560.10050487998303981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)