FastQCFastQC Report
Fri 29 Jan 2021
000000000-JHLT4_l01_n02_1800_d04_redo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JHLT4_l01_n02_1800_d04_redo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120862
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA1710.14148367559696184No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG1640.1356919461865599No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA1500.12410848736575598No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA1360.1125250285449521No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC1330.11004285879763698No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG1330.11004285879763698No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT1280.10590590921877843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)