FastQCFastQC Report
Fri 29 Jan 2021
000000000-JHLT4_l01_n01_1805_d06_redo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JHLT4_l01_n01_1805_d06_redo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254714
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG5230.20532832902785086No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT4150.16292783278500592No Hit
GAGCAAAAGCAGAACGGATAAGCAAGAGTAGGCTGGATAAGACGCGCCAG3990.15664627778606594No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGCATTTTTTTCTGCGCCAATGCA3970.15586108341119842No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA3750.14722394528765595No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG3170.1244533084164985No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCT3020.11856435060499226No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGT3000.11777915623012478No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACATCCGGCCTA2840.1114976012311848No Hit
GCTTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCAC2810.11031980966888352No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG2790.10953461529401604No Hit
CTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG2790.10953461529401604No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACATCCGGC2740.10757162935684729No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA2730.10717903216941353No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC2700.10600124060711229No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA2570.10089747717047354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)