FastQCFastQC Report
Fri 29 Jan 2021
000000000-JHLT4_l01_n01_1800_d04_redo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JHLT4_l01_n01_1800_d04_redo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120862
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG1950.1613410335754828No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA1580.13072760669192962No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA1560.12907282686038624No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA1530.1265906571130711No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG1510.12493587728152769No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT1440.11914414787112573No Hit
CTTCTATATCTCAAATAAGTCTGAAAAGAAATTTGCTTTTCTCTCAGGTG1340.11087024871340868No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG1310.10838807896609355No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC1270.10507851930300674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)