FastQCFastQC Report
Fri 29 Jan 2021
000000000-JHLT4_l01_n01_1788_d10_redo.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JHLT4_l01_n01_1788_d10_redo.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302444
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG18970.6272235521286585No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA18380.6077158085463755No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGCATTTTTTTCTGCGCCAATGCA15160.5012498181481531No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT15060.49794342093081695No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA14160.468185845974792No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGCATTTTTTTCTGCGCCAATGCA10320.3412201928290857No Hit
GTCTTCCGACTGGGCTCTTCGCTTAATTTGATGCCTGGCAGTTCCCTACT10260.33923635449868406No Hit
GTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCAAT10220.3379137956117496No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG9990.3303090820118766No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC9910.3276639642380077No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG9830.3250188464641388No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA9760.32270436841200356No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA9690.32038989035986826No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA9070.2998902276123845No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA8990.29724510983851554No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT8150.26947137321289233No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT7510.24831043102194125No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC7400.24467339408287153No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG7390.24434275436113792No Hit
GTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGCTTCGGCGTTG7230.23905251881340014No Hit
CTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG7060.23343164354392879No Hit
GAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCA6860.2268188491092566No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG6750.22318181217018687No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA6620.21888349578764996No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT6370.21061750274430968No Hit
GTCCTGTTCTGACCTGTCGATTTAATCTGTATCAGGCTGAAAATCTTCTC6360.21028686302257607No Hit
GCCTTCTGCTTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAG6290.20797238497044082No Hit
GGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCA5970.1973919138749653No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT5910.1954080755445636No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT5830.19276295777069474No Hit
CCTTCTGCTTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGA5790.1914403988837603No Hit
GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC5350.17689225112748147No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG5300.17523905251881342No Hit
TCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGT5220.17259393474494453No Hit
GAATTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCA5150.17027945669280925No Hit
GTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTG5140.16994881697107564No Hit
GAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAG5050.16697305947547314No Hit
GTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAG4950.16366666225813706No Hit
GCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCA4910.16234410337120259No Hit
CATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAT4770.15771514726693206No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT4730.15639258837999762No Hit
GGGTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTG4670.15440875004959595No Hit
TAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATG4640.15341683088439514No Hit
GAGCAAAAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAG4560.15077171311052626No Hit
GTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC4520.14944915422359178No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGA4450.14713467617145654No Hit
AACATCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTT4410.1458121172845221No Hit
CCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGG4370.14448955839758768No Hit
GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC4350.14382827895412043No Hit
AGCGAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCG4310.142505720067186No Hit
GGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT4300.1421750803454524No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA4270.14118316118025154No Hit
GGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGT4270.14118316118025154No Hit
AAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGAC4260.14085252145851793No Hit
GTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTA4250.14052188173678432No Hit
CGCTACGGCCGCCAGGCAAATTCTGTTTTATCAACACGTCCTGTTCTGAC4210.1391993228498499No Hit
CAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCA4210.1391993228498499No Hit
GAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAG4200.13886868312811626No Hit
GCTCTTCGCTTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAG4070.13457036674557935No Hit
GCCCGGTAGCTAAATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACG4070.13457036674557935No Hit
GAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGT3960.13093332980650962No Hit
TGAGAGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGT3950.130602690084776No Hit
TTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATC3940.1302720503630424No Hit
GTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAG3890.12861885175437437No Hit
GTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCC3820.1263043737022391No Hit
GAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCG3810.1259737339805055No Hit
CTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACG3780.12498181481530464No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGC3770.12465117509357104No Hit
GCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCAC3750.12398989565010382No Hit
GTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCAT3750.12398989565010382No Hit
GGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAACAC3680.12167541759796854No Hit
GGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCG3620.11969157926756688No Hit
GTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGT3610.11936093954583327No Hit
TATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGA3580.11836902038063245No Hit
ATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAC3540.117046461493698No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG3530.11671582177196439No Hit
GGAGTGGACGCATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCC3500.11572390260676357No Hit
GGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCG3420.11307878483289469No Hit
CCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG3400.11241750538942748No Hit
GCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCT3380.11175622594596024No Hit
AGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATT3360.11109494650249302No Hit
CTCCTTGAGAGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG3340.11043366705902581No Hit
GAGTAGGCTGGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCA3340.11043366705902581No Hit
GTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAAC3260.10778854928515692No Hit
CCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCCTCA3260.10778854928515692No Hit
GTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCC3260.10778854928515692No Hit
ACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATG3260.10778854928515692No Hit
ATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAA3260.10778854928515692No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT3250.10745790956342331No Hit
TCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCA3240.10712726984168972No Hit
GGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACC3220.10646599039822247No Hit
GAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAG3200.10580471095475526No Hit
GGACCCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTT3200.10580471095475526No Hit
GTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGC3130.10349023290261998No Hit
GCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGC3120.10315959318088637No Hit
GAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGA3030.10018383568528388No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)