FastQCFastQC Report
Thu 19 Nov 2020
000000000-JCJTB_l01_n02_4.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJTB_l01_n02_4.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126472
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCC8350.026.407734245
TCTCGGG12750.024.980911245
TCGCCGG13850.016.8054245
TAGATCC19700.016.789688245
GCGTCGG9653.1905074E-916.502932245
CGGTGGG16300.014.279437245
CGTATCC27850.012.316234245
GCGCTCC7350.004008379311.666908245
AGAGCGC18901.4551915E-911.018747245
GCCGTAC12852.3086926E-510.486599245
GTTCTCG17054.8480797E-710.058859245
CTATTGG17406.24387E-47.74423171
CATATCG19202.1238001E-47.65622856
GAACTTC27456.2396794E-77.58649975
ACTCACC32702.2670065E-87.49251245
CTTGAAG30153.5288576E-77.31341271
CGTATAC19100.00168321677.0549541
CTACCCC26051.8600196E-57.053889245
CTTACGT17650.00612015326.94049021
CTATTGA35359.48894E-86.93067361
Produced by FastQC (version 0.11.5)