FastQCFastQC Report
Thu 19 Nov 2020
000000000-JCJTB_l01_n02_3.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJTB_l01_n02_3.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704503
Sequences flagged as poor quality0
Sequence length251
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG4700.039.095745245
TAGATCC6450.028.48837245
GCGTCGG3504.4205444E-727.999998245
GTGGTCC3954.0814484E-827.91139245
TCGCCGG4758.543793E-925.789474245
CGGTGGG4752.4326982E-723.210527245
TCGCTCC4705.749078E-620.851065245
CGTATCC7151.3897079E-920.55944245
AAAGAGG20400.015.612745245
AGAGTGG6650.001891122512.894736245
CACTCCC7904.8731928E-412.405063245
CTATGTA8458.579314E-411.5976331
ATAGTGT8250.00945668110.3939393
GAATTAG11250.0091942418.7111111
TAAGAAT15952.4431708E-48.4482763
CCTCTCC19002.0601115E-58.381579245
CTCCCTC16704.0095142E-48.068862245
CCCCTCC16854.4139536E-47.9970326245
CCTCCCC15600.00182818577.852564245
CCTTTCC14700.0094164717.4999995245
Produced by FastQC (version 0.11.5)