FastQCFastQC Report
Thu 19 Nov 2020
000000000-JCJTB_l01_n02_2.5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJTB_l01_n02_2.5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912486
Sequences flagged as poor quality0
Sequence length251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG22400.034.999905245
GCGTCGG16800.029.166588245
CGGTGGG25050.025.42907245
TCGCCGG22950.023.485773245
GTGGTCC18400.022.635807245
CGTATCC36500.017.452005245
AGAGCGC27050.012.227323245
TAGATCC39600.011.445676245
GTCGCCC13754.8670718E-59.7999735245
CGCCGTC22552.5465852E-99.778243245
AAAGAGG104500.08.322944245
GTCGTGG21801.3497152E-57.866951245
GCGGTCG14450.0080958327.6297364245
ACGCTCC19953.305635E-47.3684006245
GTGTCGT25501.3804136E-57.2058625245
GTTCGGA20955.7910976E-47.016687245
CTAATCG17500.00563647136.99998041
CGGTGGC30252.1277416E-56.4793215245
AGAGTGG30602.5190133E-56.405211245
TCTCCCC59405.456968E-126.3930798245
Produced by FastQC (version 0.11.5)