FastQCFastQC Report
Thu 19 Nov 2020
000000000-JCJTB_l01_n02_2.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJTB_l01_n02_2.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326991
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG8750.053.199997245
TCGCCGG9000.027.222221245
CGTATCC13800.025.742752245
GTGGTCC6259.094947E-1225.48245
GCGTCGG6004.141839E-922.458334245
TAGATCC13950.020.197132245
CGGTGGG9803.910827E-916.25245
CGGTGGC11801.125829E-410.381356245
TCCCCCC23953.632911E-46.649269245
CTTCCCC24103.9201239E-46.607884245
ACTCCTC20650.0038216656.5254235245
AAAGAGG36201.0458098E-66.429558245
CCCCCAG4600.00167149986.391304235-239
CATCTCC23650.00228311876.215645245
TCCTCCC26009.827514E-46.125245
CCCCCTC29150.00384303075.4631214245
TCTCTCC35550.00130531815.168776245
AATGGCA30950.00776501745.14539581
CCTCTCC33450.0034278335.127055245