FastQCFastQC Report
Thu 19 Nov 2020
000000000-JCJTB_l01_n01_4.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJTB_l01_n01_4.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126472
Sequences flagged as poor quality0
Sequence length251
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGACG8606.9419E-512.8197361
CGGGGTC11205.0226336E-612.03121959
CATGAGG10000.00349375429.7999761
GTATAGG11200.0088775388.7499781
CTCAGAG12650.00244232668.7153941
CTTACGG11250.0092070858.7110891
CTACGAA14106.73418E-48.6879221
CTAGAAT21301.0669701E-68.6267391
TCCGGGG14609.5201E-48.390397
ATGGGAT18001.004435E-48.1666473
TGAGACT14300.0073716827.7097718
GAGAGGT14550.0086071217.57730054
TCTACCC31559.5906216E-87.377334245
CGTTCAT18550.0012350767.2641336
ATGGTCT17250.00489909957.1014313
CTACAGT19350.00193111446.96380661
CACACCC41054.270987E-96.863725245
GCATTGT21507.7777816E-46.83719252
CTTAAAT36351.5777005E-76.74001071
CTCAATA32308.042141E-66.4473521