FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n02_HRBB4LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n02_HRBB4LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAACGACTTGGCGCTCTCTATGCAAGATCGGAAGAGCGTCGTGTAGGG313.043478260869565Illumina Single End PCR Primer 1 (100% over 24bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGAAGTGTGTAGATCT28.695652173913043Illumina Single End PCR Primer 1 (96% over 33bp)
GACCGGAAAAGCGTCCTGTCGGGGAAGAGGGTTTTTAATGTTGTGTACTC14.3478260869565215No Hit
GCCCCGCAAAAAGGTGTGTCGGGGCAACGCGCTTCGTATTATGCCTCCCA14.3478260869565215No Hit
ACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGAAGTGTGGAG14.3478260869565215Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGGAGAGCGTCCGGTAGGGGGAGAGGGGTTTAAGCGGGGAGGATAC14.3478260869565215No Hit
ACAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGAAGTGTG14.3478260869565215Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGGAAAGCGGCGGGGGGGGGAAAAGGGGTTTGAGTGGGGTGGGCTC14.3478260869565215No Hit
GATTCCATAGGACACGTGGTAAGTGAGAGATTTTTTACATTTTAATTATT14.3478260869565215No Hit
GATCGGGAGAGCGGCGGGGGGGGGAAAAGGGGTTTTAAAGTGGGGGAATT14.3478260869565215No Hit
GATCCGGAGAGCGGCGGGGAGGGGAAGAGGGGTTTAAATGTGGTGAATTT14.3478260869565215No Hit
GATCGGGAGAGCGTCGGGGAGGGGAAAAGTGGTTTAAATGTGTTGAATTT14.3478260869565215No Hit
GAACCGGAAAATGGCGGGTAAGGAAAAAGGGGTTTAAATGTGTAAAGTAC14.3478260869565215No Hit
ACAAGACGGGAGTGGACATTGGTAGTACAGGCGGAAAGGCTACAAGGAGG14.3478260869565215No Hit
GTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGAAGTGTGT14.3478260869565215Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGGAAAGGGGTGGGGGAGGGAAAGGGGGTTGAAAGGGGTTGAGAAT14.3478260869565215No Hit
AAAAGTATGAATCGACAGAAGCCTGTGTAATAAAACAGGATCGGGAGGGT14.3478260869565215No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGAAGTGTGTAGATCC14.3478260869565215Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGGAGAGGGGCGGGGTGGGGAAAAGGGTTTGGAGTGTGGGGGAATC14.3478260869565215No Hit
GTTCTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGAAGTGTGT14.3478260869565215Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)