FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n02_HRBB3EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n02_HRBB3EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGACCTGGTGTAGATCT492.451225612806403Illumina Single End PCR Primer 1 (97% over 36bp)
GTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGACCTGGTGT20.10005002501250625Illumina Single End PCR Primer 1 (97% over 36bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCTTCCGATCTAGAT20.10005002501250625Illumina Single End PCR Primer 1 (96% over 33bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG20.10005002501250625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)