FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n02_HRBB2LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n02_HRBB2LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATGCGGTGTAGATCT684.712404712404712Illumina Single End PCR Primer 1 (96% over 33bp)
GTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATGCGGTGT140.9702009702009702Illumina Single End PCR Primer 1 (96% over 33bp)
GTTCTACAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATG80.5544005544005544Illumina Single End PCR Primer 1 (96% over 33bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATGCGGTGTAGAT50.3465003465003465Illumina Single End PCR Primer 1 (96% over 33bp)
TGCATAGAGAGCGCCAAGTCGTTGCGAGATCGGAAGAGCGTCGTGTAGGG40.2772002772002772Illumina Single End PCR Primer 1 (100% over 24bp)
CGCAACGACTTGGCGCTCTCTATGCAAGATCGGAAGAGCGTCGTGTAGGG30.2079002079002079Illumina Single End PCR Primer 1 (100% over 24bp)
GAACTCCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAT20.1386001386001386Illumina Single End PCR Primer 1 (96% over 33bp)
GTTCTGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATGCGG20.1386001386001386Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGTAAAGGGGGGGAGAATTC20.1386001386001386No Hit
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATGCGGT20.1386001386001386Illumina Single End PCR Primer 1 (96% over 33bp)
GTTCTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATGCGGTGT20.1386001386001386Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)