FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n02_HRBB1LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n02_HRBB1LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTGCAAGAGCGGCGTGCGTGGAAAGAGAGGTACAGGCCTTTGTAGATC10.8No Hit
GATCGGGAAAGGGCCGGGGGGGGGAAGAGGGGCAAGGTCCCGGTGAATAT10.8No Hit
GAACCATTCCCTGGATGGCGACCACTGATCCGGATGTTCAGGATGTGGGA10.8No Hit
GATCGGGAGAGCGGCGTGGCGGGGAAGAGGGTCCTCTTCCTGTGGAGTTC10.8No Hit
TCTGATTTACGATCCGACTAAACCATTGTATTCATTGAAATTGAATCTTT10.8No Hit
GAACGGGAGAGCGGCGTGTAGGGGAAAAGGGGCAAACTTCGGGAGAAGTA10.8No Hit
GAACGGGAGAGGGGCGGGTAGGGGAAGAGTGGCAAGATTGTGTTGAGGTT10.8No Hit
GAACAGGAGAGAGGCGGGGAGGGGAAAAGGGGCAAGGAGACGGAGGGTCC10.8No Hit
GATAGGAGGAATGGTGTGTAGGGGAAAAAGGTGACTACCCGGGTGGTCGA10.8No Hit
GATCCGGAGAGCGGCGTGTTGGGGAAGAGGGGCAAGTCCCGGTAGGGTTT10.8No Hit
GATCGGAAAAGGCGCCCGTAGGGGAAGAGTGACAAGCCTCCGCAGGTGTC10.8No Hit
GATCTGAAAGGACTCCTGGAAGGGAAGAACGGTCTACCCGTCGATAAATT10.8No Hit
GATTAGGTTAGAGCTGCGTAGGGGAAGGGAGTTATTACTGTCTTCTCGTG10.8No Hit
CATCGCGATAGCGTCGGGGAGGGGAAGAGTGTGATTTCCAGGGAGCTCTT10.8No Hit
GATCGGGAGAGGGTCGGGGAGGGGAAGAGAGGGATGCCCCTGGAGGACTC10.8No Hit
GCTCCGAAAAGCGCCGTGTAGGGAAAGAGAGCCAAACCCCGGAATAAAAC10.8No Hit
GTTCGGGAAAGAGGCGGGTAGGGGAAGAAGTGCCATTTCCCCTACGTAAG10.8No Hit
GAGTGGGGGGGCGGCGGGAGGGGAAAAAGGGGGAACCGTGTGGGGACAAG10.8No Hit
GATCGGGAGAGCGGCGGGGAGGGGAAAAGGGGCAAGGCCGGGGTGAAAAT10.8No Hit
GGTCAGCAGAAGGATGTGGAGGGGAAGAGTGGCCTTTTCCGCTTAGCTTC10.8No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATGCTCGTGTAGATCT10.8Illumina Single End PCR Primer 1 (96% over 33bp)
GCATGGGAGAGGACAGCGTAGGGGAAAAGGGACAAGCCGCGGGGGGCGAC10.8No Hit
GATTCGAAGGGGGGCGTGCTGGGGAAGAGCGGCCTCCTCCAGTTCATCCT10.8No Hit
GATTCGGAGAACGCCGTTTTGGGAAATAGTGACAACGTCGTGGTGGTCCA10.8No Hit
GATCGGAAGAAGGGCGGGGTGGGGAAAAGAGGAACACACGCGGACAATCT10.8No Hit
GACCGGGAGAGGGGCGTGGAGGGGAAGAGGTGTATACCCGTGGTAGAAAG10.8No Hit
GATCGGGAGGGGGGCGGGTAGGGGAAAAGGGGGATGCTCGGGGGGGAAAT10.8No Hit
GATCGGGAGAGGGGCGGGTAGGGGAAGAGGGGTCATCTCCCTTTGAAAAC10.8No Hit
GAGGGGGCACTCGGCGGTGACGCGACGGCGGCGCGAGGGGGAGGTGAGAT10.8No Hit
AAACCGAACGGCGGTCTTTAGGGCAAAAAGGGACAACGTCGAGCGGAAGA10.8No Hit
GATCGGGAGAGCGGCGGGTAGGGGAAAAGGGGCATTACCCGGGTGATATG10.8No Hit
GATCGGAAGAGGGTCGTGGAGGGGAAAAGGGGCCAGCCCGGGGAGAAAAC10.8No Hit
GATCCGTAGAGGGGCGGGGTGGGGAAGTGTGGGAGTCCGCTGGTGATCAT10.8No Hit
GAACCGGAGAGCGTCGGGTGGGGGAAGAGAGTCAAAGGTCGGAAGAGCGT10.8No Hit
GATCCGGAGAGCGTCGGGTAGGGGAAGAGGGTCACACGCCGGGTGAAATT10.8No Hit
GATCGGGAGAGGGTCCGGGAGGGGAAGAGGGTCATTCTCCGGGGGAAATC10.8No Hit
GAACGGGAAAGAGGGGGGGAGGGGAAGGGGGGCAAAGAGAGGGCAGAAGC10.8No Hit
GTTCAGTGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATGCT10.8Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGGAGAGGGCCGTGGAAGGGAAAGATGGCATTATCGGCGGAAGGCT10.8No Hit
GGTGCGGAGAGCCTTGTGTAAGGGAAAAGAAGCTCGCGTTTGTATATCAG10.8No Hit
GATCGGAAGAGCGGCGGGGAGGGAAAGAGGGGCATGTGCGCGGGAAAGTC10.8No Hit
GATCGGGAGAGGGGCGGGGGGGGGAAGAGGGGCATGCCCGGGGAGAGATT10.8No Hit
GATCCGGAGAGCGGCGGGGAGGGGAAAAGGGGCACACTCCCGGTGATAAT10.8No Hit
GATCGGGAGAGCGTCGGGGAGGGGAAAAGGGGCAATCCCCGGGTGAGCTT10.8No Hit
GATCGGGAGAGGGGTGGGGTGGGGAAGAGGGGGATTTTTCGTTTGGGTTC10.8No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGCAAAGCCCGGGGGAAAAT10.8No Hit
GAACGGGAGAGGGGTGGGTAGGGGAAGAGGGGCATTCCCGCGGGGGGATA10.8No Hit
AGCGGGCGTCCCGCTTCATCCTGGTGAGCATCAGCAAGATCTGAAGAGCG10.8No Hit
GATATGAAGAGGGGGGTGTAGGGAAAGAGAGGCAAGCTCCGGGAGGGATC10.8No Hit
GATCCGGAGGGCGGGGTGGAGGGAAAAAGGGGCCTGCTTCTTCGTGATTC10.8No Hit
GATCGGGAAAGGGGCCGGGAGGGGAAGAGGGGCATTGCCCGGGTGGAGCC10.8No Hit
GATCGGAAGAGGGGTGGGTAGGGGAAGAGGGTCAATCCCGGGCTCGGACG10.8No Hit
GATCCGAATAGACGCGTGTTGGGGAAGGGGGTTCTGGGTATGTTGTGAGC10.8No Hit
AAGTTAACACGCTCAAATACCACAGGATACAAAAACAGATCTGTCTCTGC10.8No Hit
GGTCGGGAGAGCGTCCGGGGGGGGAATGGGGTGAAGGCCCAGGGGGTCGT10.8No Hit
GATTCGAAGAGGGGCGGGGAGGGGAAAAGGGGTAATGCCCGGTAGGTAAT10.8No Hit
GATCGGGAGAGCGGCGTGGAGGGGAAGAGGGGCATCTCCCGGTGGGTCTT10.8No Hit
TACCATCCAATGCAATGTATACGTATGAGATCGTAAGAGCGTCGTGTAGG10.8Illumina Single End PCR Primer 1 (95% over 23bp)
GATCCGAGAAACGTCGGTTGAGGGAAAAGGGACTATCTCCGGTGTATGTC10.8No Hit
GGATAGCAGAAACGGGTGGAGGGGAAAAGGGGTCTCCAGCGGTAAGGGGA10.8No Hit
GATTGGTGGAGCGGCCGGTAGGGGAAGAGGGGCCAGCCCCTGCTAGGCAC10.8No Hit
GCCCACAGACGCGGCGTGGTGGGGGAAAGGGTCTTAACCGTGTGAATTAT10.8No Hit
CTCGTGATGTTTCTTTGTGTGTGGATCAAATGTTGCAGTGAATAGATCGG10.8No Hit
GATCGGAAGAGGGGAGGGTAGGGGAAGAGGGGCTTAGGTCTGTGGAAATT10.8No Hit
GATCCGAAGGGCGTCGGGGAGGGGAAGAGAGGCATGTTCCGGGAGGTAGT10.8No Hit
AATAAAGGTACAACAACTTGATATTGCACAATAGACCTTTGCTTACACCG10.8No Hit
GAACGGGAGAGGGGCGGGTAGGGGAAGAGGGGGCATATCCGGTGAGGCGT10.8No Hit
GTTCTGCAGAGCGCCGGGTCGGGACAAAGGGTCCTTCCCGTGTAGGGTTT10.8No Hit
GATTCGGAAAGCGTCGTGTTTGGGAAGAGGGACCCTGCCCCCTGGTATAC10.8No Hit
ATCTCCCTTCCCTTGCGACGCTCCACGTCTTGCTCCTTCTTTGCGTTTTT10.8No Hit
GATCCGGAGAGGGGCCTGTAGGGGAAGAGTGGTCTCACCCCTTGTGTTTA10.8No Hit
GAGGCTCCTCCCGATAATAGTGTGAGATCATAAAGATAATCGGAAAATAC10.8No Hit
GATGAAGAGAGAGTAGTGTAGGGGAAGAGGGTTACAGGGACAATCAAATT10.8No Hit
GTTTGGGAGAGGCGTGGGGAGGGGAAGAGAGCTCAAGATCGGCAGAGCTT10.8No Hit
GCTGAGGAGGAGGGAGGGGGAGGGAAAAGGGGCAAGTCCGGGGGAGAAAG10.8No Hit
GTTCTGGAGGGTGGCGTGTAGGGAAAAAGTGGCGGAGCAGGGGCGTGTAG10.8No Hit
GTTTGGGATTGCTTCGGTTAGGGGAAAAGGGTCAATTTGTCGGCTCATCC10.8No Hit
GATCCGAAGAGCGGCGTGGAGGGGAAGAGAGGGACGCTCGGGGTAGAATC10.8No Hit
GAACGGCAGAGCGTCGTTTTGGGGAAAAGTATATATTCACCGGAAATCTC10.8No Hit
GATCCGGAGAGCGTCGGGTGGGGGAAGGGAGGTAGAGGCCGGGAGAGATA10.8No Hit
CGCTTGGCCTTGTCGGGTTCCTCAGCACAGTGGCGCTGGCGCGCTTCATT10.8No Hit
GTACGGCAGAGGGGCGGGGAGGGGAAAGGGGGAAAGGTCCGTGTAAGTGA10.8No Hit
TCCCAGGATGATTTTTCGGGTGGGAAAAGGGGCCAGGCCGGATTAGCAAA10.8No Hit
GATCCGAAGAGCGTCCTGTTGGGGAAGAGTGGCATTCCCCCCTTGTTATC10.8No Hit
GAACGGGAGAGGGGCGGGAGGGGAAGAGGGGCATAGCCGGGGGAGGATCG10.8No Hit
GATTGGGAGAGGGTCTTGGTGGGAAAGAGTGGGCTATTCCCGGTGGTTTG10.8No Hit
ACATAACCTTTACCTCTGCCGTCTGGCTCATACCTCGACCTTGCTAAAGG10.8No Hit
GATCCGCAGAGCGGCGGGGTGGGGAAAAGTGTCCACTTTCCGGAGATCAT10.8No Hit
GATCCGGAGAGGGGCGGGGAGGGGAAGAGGGGCAAGCCCCCGTTGGAAAT10.8No Hit
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGGCAAGCTCGGGGGGGAATT10.8Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAAAGGGGGGGGGAGGGGAAGAGGGGCAAGCGCCCGGGAGGATC10.8No Hit
GACCCGAACAGTCGCGCTGAAGGAAAACGGAACAAACCCTTTTTACGATT10.8No Hit
GATCGGGAGAGCGGCGGGGTGGGGAAAAGGGGGCTGGCCCCGGGGAATTC10.8No Hit
ACAAGCGGCGCACCTCCGTTCTGTCTTTGAGATGAAGATCGGGAGAGGGT10.8No Hit
GTACCGTAAGGCGTCGGGGTTGGGGAAAAGGGCAAGCTACATGGGATATA10.8No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGCAAGCGCGGGGGGGAAAC10.8No Hit
GATCGGAAGAGGGGCGGGTAGGGGAAGAGGGGCCTGGCCGGGGGGAGATC10.8No Hit
TTCCCCATCCTGAGGGGGTTGTCGTGCCGTCTCGAGATCGGAAGAGCGTC10.8No Hit
GATTGGGAGAGCGCCGGGTATGGGAAGAGGGTCCTGGCCGCGGTTGTGCC10.8No Hit
ACGACCGCGCGCCGTTCCACCCCAGACAGGAAAAGCGGCGGCGCGGGCCC10.8No Hit
GATCCGGAGAGGGTCGGGGAGGGGAAGAGTGGTCTTTTCCGGTCTGTTTT10.8No Hit
GATCGGGAGAGCGTCGTGTAGGGGAAAAGGGTCATGGCCCGGGTAAAATT10.8Illumina Single End PCR Primer 1 (95% over 23bp)
GGCCCGCGCAGACCGGCGGAGGGGAAAGGGGTTATTCCAGGGCATCTAGG10.8No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAAAGGGGGAATCGCGCGGCGAACTT10.8No Hit
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGGCAAGCCCGGGGGGAGAAT10.8No Hit
GAACGGAAGAGAGGCGGGGCGGGGAAGAGCGTTCCAGCCGCGGAGACTTG10.8No Hit
GGGACGAAGAGGCTCGCGGTAGGAAAGAGCGAAATTTCCTGGTAGATCTG10.8No Hit
GATCCGGAGAGCGGCGTGGAGGGGAAGAGGGGCATGGCCGTGTTGGAAAC10.8No Hit
GCTTTGGGGAGAGGCGTGGGTGGGAAGAGGGGCATTTCGGCGTGGAGTCC10.8No Hit
AGATCGGACGAGCGTCGTGTAGGGAAAGAGTGACATGGTCGGGGAGAGCT10.8Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGGAGAGGGGCGTGGAGGGGAAGAGGGGCATGCCCCGGGGGAGATA10.8No Hit
GTAGGCCTCCGTTTCCTCCGTGGCAATAGGGGTTTTGCGCTCGGGCGAGC10.8No Hit
GTTCAGGATAGGGCTCGGTAGGGGAAGAGTTCTAGCTGTCCCAACCAGAT10.8No Hit
TGGCACATCATCAGATCGCCTCAGATCGGAAGAGCGTCGTGTAGGGAAAG10.8Illumina Single End PCR Primer 1 (100% over 28bp)
GATCGGGAAAGGGGCCTGGAGGGGAAGAGGGTCATTCGCCGGGGTAGATT10.8No Hit
GATCGGAAGAGGGGCGGGTAGGGGAAAAGGGGCAAGCCCGTGGTGAAATC10.8No Hit
GTCTGGTAGAGCGGCGGGTAGGGGAAGAGAGTCGCACACGCTTTTATATA10.8No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGCAAATCTGCGGCGGATTC10.8No Hit
GATTTGCAGAGCGTCGCGTGGGGGAAGGGTGGCTCTCTCCCTCTGCGATC10.8No Hit
GAGCTGGAGGGCGATCAGGAGGGGAAAAGGGTCAAGCACCACTTAGAATG10.8No Hit
GATCCGAAAAGCGGCGGGGAGGGGAAAAGGGTCAGTGCCCCGTTTAGATT10.8No Hit
GATCGGGAAAGCGTCGGGTAGGGGAAGAGGGTCAAGCTCCCGGTGGACTA10.8No Hit
GATCGGAAGAGCGTCGGGGAGGGGAAGAGGGGCATTCTCCCGGAGGAGTC10.8No Hit
GCTTCGGAGAGGGTTGGGGAGGGGAAGAGGGTCTCTCCCCTCGGAAGATC10.8No Hit
GCATCGGAGAGGGCTGTGGGGGGAGAGAGGTTCTATTCCGCGGTATCAGA10.8No Hit

[WARN]Adapter Content

Adapter graph