FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n01_HRBB4LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n01_HRBB4LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATCTCGTATG1043.47826086956522TruSeq Adapter, Index 9 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATCTCGTAT313.043478260869565TruSeq Adapter, Index 9 (97% over 37bp)
TGCATAGAGAGCGCCAAGTCGTTGCGAGATCGGAAGAGCACACGTCTGAA313.043478260869565Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATC14.3478260869565215TruSeq Adapter, Index 9 (97% over 37bp)
GTATTCGGATCTCGTATGCCGTCTTCTGCTTGAAAGAAAAAGCACACGTC14.3478260869565215Illumina PCR Primer Index 9 (96% over 28bp)
CAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGCATC14.3478260869565215TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGACACGCTCCGTATTTCGTATG14.3478260869565215TruSeq Adapter, Index 6 (97% over 35bp)
ACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATCTC14.3478260869565215TruSeq Adapter, Index 9 (97% over 37bp)
CACGTCTGAACTCCAGTCACGCTCCGTATCTCGTATGCCGTCTTCTGCTT14.3478260869565215TruSeq Adapter, Index 18 (96% over 28bp)
GTTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTAT14.3478260869565215TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)