FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n01_HRBB3LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n01_HRBB3LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG31271.72413793103448TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTAT235.287356321839081TruSeq Adapter, Index 20 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC61.3793103448275863TruSeq Adapter, Index 20 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG51.1494252873563218TruSeq Adapter, Index 21 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATATCGTATG40.9195402298850575TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC30.6896551724137931TruSeq Adapter, Index 21 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATACCTCGTATG20.45977011494252873TruSeq Adapter, Index 20 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCG20.45977011494252873TruSeq Adapter, Index 20 (97% over 35bp)
GATCGTAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG20.45977011494252873TruSeq Adapter, Index 21 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTC20.45977011494252873TruSeq Adapter, Index 20 (96% over 33bp)
CAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATC20.45977011494252873TruSeq Adapter, Index 20 (97% over 38bp)
AGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTCTTCT20.45977011494252873TruSeq Adapter, Index 20 (96% over 29bp)
GATCGGAAGAACACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG10.22988505747126436TruSeq Adapter, Index 21 (97% over 36bp)
GAGTTCAGACGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT10.22988505747126436TruSeq Adapter, Index 9 (97% over 36bp)
AACCATGGCCTCTCTCTCAGTCAGTAACCATGGTCTCTCTCTCAGTCTTT10.22988505747126436No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGCATATCTCGTATG10.22988505747126436TruSeq Adapter, Index 27 (97% over 38bp)
AGTGAAACTTAATCTCAAAAAAAAAAAAAAAAACCTCAAAAAAAAAAACC10.22988505747126436No Hit
GAGCACACGTCTGAACTCCAGTCACGTTGCATATTTCGTATGCCGTCTTC10.22988505747126436TruSeq Adapter, Index 20 (96% over 30bp)
TGAAGCTTGTAAGGCAGGTTAGAGATCGGAAGAGCACACGTCTGAACTCC10.22988505747126436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTACATATCTCGTATG10.22988505747126436TruSeq Adapter, Index 21 (97% over 38bp)
CGGCCTAACTAGGGAACCAGCCGATGCAGGCAAGAAAAGGAAAAGATCGG10.22988505747126436No Hit
GATCGGAAGAGACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC10.22988505747126436TruSeq Adapter, Index 21 (96% over 25bp)
CAAGGAGGATGCTGCGGCTTCACGCAGAAATCAGATCTGGAAATGTAATG10.22988505747126436No Hit
CGCAACGACTTGGCGCTCTCTATGCAAGATCGGAAGAGCACACGTCTGAA10.22988505747126436Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATC10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC10.22988505747126436TruSeq Adapter, Index 20 (96% over 33bp)
GATCGGAAGAGACACGTCTGAACTCCAGTCACGTTGCATATCCCGTATGC10.22988505747126436TruSeq Adapter, Index 21 (96% over 25bp)
CCTGTCTTTCGTCTGAACTCCAGTCACAGACTCCATCTCGTATGCCGTCT10.22988505747126436TruSeq Adapter, Index 4 (95% over 22bp)
TCTGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCC10.22988505747126436TruSeq Adapter, Index 21 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCCACGTTGCATATCTCGTATG10.22988505747126436TruSeq Adapter, Index 21 (97% over 36bp)
TACCCACGACACTGTATGAAGTAGAAGTAATCCCATGAGGCTGGCAGCCA10.22988505747126436No Hit
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACGTTGCATATCTCGTAT10.22988505747126436Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GATTCGGAAGAGCACAACGTCTGAACTCCAGTCACGTTGCATATCTCGTA10.22988505747126436TruSeq Adapter, Index 20 (95% over 24bp)
GTTCTGTAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTG10.22988505747126436TruSeq Adapter, Index 12 (97% over 37bp)
GGCTACGGGAGAGGAGGCGGCTGCTGCAGCGAGAGGGCACACGTCTGAAC10.22988505747126436No Hit
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGT10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCAACACGTCTGAACTCCAGTCACGTTGCATATCTCGTAT10.22988505747126436TruSeq Adapter, Index 20 (96% over 26bp)
GACCGAAGATCAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTA10.22988505747126436TruSeq Adapter, Index 21 (96% over 28bp)
TAGATCGGAAGAGCACACGTCTGAATTCCAGTCACGCTGCACATCTCGAA10.22988505747126436TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCCACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC10.22988505747126436TruSeq Adapter, Index 20 (96% over 25bp)
TGCATAGAGAGCGCCAAGTCGTTGCGAGATCGGAAGAGCACACGTCTGAA10.22988505747126436Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCAAGATATCAAGGAATAGAGTAACTAATGCAAATTGGCCATAAGAAA10.22988505747126436No Hit
GATCGGAAGAGCAAACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG10.22988505747126436TruSeq Adapter, Index 21 (97% over 36bp)
CGCACCACGGTGACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10.22988505747126436Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCGGAAGAGCAGACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG10.22988505747126436TruSeq Adapter, Index 21 (97% over 36bp)
TATCGCAACTAAAGCCATACCATTGGCAGAACATTTTTAAATTGATCTAT10.22988505747126436No Hit
AGATCGGAAGAGCACACGTCTGAATTCCAGTCACGTTGCATATCTCGTAT10.22988505747126436TruSeq Adapter, Index 21 (97% over 36bp)
CCGCGTAGTAATTGCGACGCCGTGGGACGACATTCCGTGCTCGGAAGAGC10.22988505747126436No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGCATG10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGT10.22988505747126436TruSeq Adapter, Index 20 (97% over 34bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATA10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG10.22988505747126436TruSeq Adapter, Index 21 (97% over 36bp)
GAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGCCTTC10.22988505747126436TruSeq Adapter, Index 20 (96% over 30bp)
AAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTCT10.22988505747126436TruSeq Adapter, Index 20 (96% over 32bp)
GTACAGTGCCTCAGATTCATCGAGATCGGAAGAGCACACGTCTGAACTCC10.22988505747126436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC10.22988505747126436TruSeq Adapter, Index 21 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCCAGTAT10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC10.22988505747126436TruSeq Adapter, Index 20 (96% over 29bp)
GTTCCCCGCGCTCGTGTACCGGAACAGCAGGCCCGCAAACAAGATCGGAA10.22988505747126436No Hit
GCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTCTTCTG10.22988505747126436TruSeq Adapter, Index 20 (96% over 28bp)
TGATGTCAGTTCAGTTGTCATCTGAGACATTCTCATCAATGATATGATGA10.22988505747126436No Hit
CATCGGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTAT10.22988505747126436TruSeq Adapter, Index 20 (97% over 34bp)
GTTCTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCAT10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCGTTTACC10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
GCGCTCAATGGTGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10.22988505747126436Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGCATATCTCGCATG10.22988505747126436TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCACATCTCGTATG10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
CGAAGTACAACAAAAGGATGAAGAGGACTTGTGAACACACTATAACTTCC10.22988505747126436No Hit
GTGCGGGAGTAAAATGTACGCATGAAAGACGATTCAACACTTAAGAAAGA10.22988505747126436No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATCTCGTATGCC10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAAGCACACGTCTGAACTCCAGTCACGTTGCATCTCGTATGCCG10.22988505747126436TruSeq Adapter, Index 20 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTCTT10.22988505747126436TruSeq Adapter, Index 20 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGTCACGTTGCATATCTCGTAT10.22988505747126436Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CATGTTCTCACTTGAATACTTTACCGACGCAGTGAGCAAGGCGCCTGGCA10.22988505747126436No Hit
ACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTACAG10.22988505747126436Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GATCGGAAGGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGC10.22988505747126436TruSeq Adapter, Index 21 (96% over 27bp)
AAAATATCGAAACTGCCGGCGCTGGTCAGCACATGCTGGCCGGGACCCTG10.22988505747126436No Hit
AACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCG10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
ACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGT10.22988505747126436No Hit
GATCGGAAGAGCACACGTATGAACTCCAGTCACGTTGCATATCTCGTATG10.22988505747126436TruSeq Adapter, Index 21 (97% over 36bp)
GAGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATC10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)
GTTCTAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCA10.22988505747126436TruSeq Adapter, Index 20 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)