FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n01_HRBB3EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n01_HRBB3EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTATG21610.805402701350674TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTAT492.451225612806403TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATATCGTATG90.4502251125562781TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATATCGTAT50.25012506253126565TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATTTCGTATG30.1500750375187594TruSeq Adapter, Index 3 (97% over 37bp)
CAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATC20.10005002501250625TruSeq Adapter, Index 3 (97% over 37bp)
GCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTATGCCGTCTTCTG20.10005002501250625TruSeq Adapter, Index 3 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATTTCGTAT20.10005002501250625TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAAGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTATGC20.10005002501250625TruSeq Adapter, Index 3 (96% over 28bp)
AGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTATGCCGTCTTCT20.10005002501250625TruSeq Adapter, Index 3 (96% over 28bp)
AGATCGGAAGAGCACACTCTTTCCCTACACGACGCTCTTCCGATCTAGAT20.10005002501250625Illumina Single End PCR Primer 1 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTATGCCG20.10005002501250625TruSeq Adapter, Index 3 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAACCTCGTAT20.10005002501250625TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)