FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n01_HRBB2LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n01_HRBB2LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG93364.65696465696466TruSeq Adapter, Index 23 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTAT674.643104643104643TruSeq Adapter, Index 23 (97% over 40bp)
CGCTACGTAGTCGAACGGTCCGATCTAAGTCACACGTCTGAACTCCAGTC443.049203049203049Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATATCGTATG161.1088011088011087TruSeq Adapter, Index 23 (97% over 40bp)
CAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATC130.9009009009009009TruSeq Adapter, Index 23 (97% over 40bp)
GAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTCTTC110.7623007623007623TruSeq Adapter, Index 23 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGC90.6237006237006237TruSeq Adapter, Index 23 (97% over 39bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTC80.5544005544005544TruSeq Adapter, Index 23 (97% over 35bp)
GCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTCTTCTG70.4851004851004851TruSeq Adapter, Index 23 (96% over 30bp)
CACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTCTTCTGCTT60.4158004158004158RNA PCR Primer, Index 32 (96% over 28bp)
GTTCTGTAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAC60.4158004158004158TruSeq Adapter, Index 23 (97% over 37bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGT60.4158004158004158TruSeq Adapter, Index 23 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATTTCGTATG60.4158004158004158TruSeq Adapter, Index 23 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCG60.4158004158004158TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTACCTCGTATG60.4158004158004158TruSeq Adapter, Index 23 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTCTT50.3465003465003465TruSeq Adapter, Index 23 (96% over 33bp)
TAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATC40.2772002772002772TruSeq Adapter, Index 23 (97% over 40bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG40.2772002772002772TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGC40.2772002772002772TruSeq Adapter, Index 7 (96% over 30bp)
CGCAACGACTTGGCGCTCTCTATGCAAGATCGGAAGAGCACACGTCTGAA40.2772002772002772Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG30.2079002079002079TruSeq Adapter, Index 7 (97% over 35bp)
AAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTCT30.2079002079002079TruSeq Adapter, Index 23 (97% over 34bp)
TGCATAGAGAGCGCCAAGTCGTTGCGAGATCGGAAGAGCACACGTCTGAA30.2079002079002079Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGC30.2079002079002079TruSeq Adapter, Index 7 (96% over 26bp)
AGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTCTTCT30.2079002079002079TruSeq Adapter, Index 23 (96% over 31bp)
CGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTCTTCTGCTTGA30.2079002079002079RNA PCR Primer, Index 32 (96% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGT20.1386001386001386TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCTCTATCTCGTATG20.1386001386001386TruSeq Adapter, Index 9 (97% over 37bp)
CACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTCTTCTGC20.1386001386001386TruSeq Adapter, Index 23 (96% over 29bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG20.1386001386001386TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCAAACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG20.1386001386001386TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGCATG20.1386001386001386TruSeq Adapter, Index 23 (97% over 40bp)
GTCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGC20.1386001386001386TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGATCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG20.1386001386001386TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAACTCTATCTCGTATG20.1386001386001386TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTATATCTCGTATG20.1386001386001386TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCCACCAACTCTATCTCGTATG20.1386001386001386TruSeq Adapter, Index 7 (97% over 35bp)
CCTACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTCTTCT20.1386001386001386TruSeq Adapter, Index 7 (95% over 24bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTA20.1386001386001386TruSeq Adapter, Index 23 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTAACCAACTCTATCTCGTATG20.1386001386001386TruSeq Adapter, Index 7 (97% over 35bp)
GTTCTGTAGAACAAATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAC20.1386001386001386TruSeq Adapter, Index 7 (97% over 35bp)
GAACTCCAGTCACCAACTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.1386001386001386TruSeq Adapter, Index 2 (96% over 27bp)
GATCGTAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG20.1386001386001386TruSeq Adapter, Index 7 (97% over 35bp)
GATCGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGC20.1386001386001386TruSeq Adapter, Index 23 (97% over 35bp)
TGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATC20.1386001386001386TruSeq Adapter, Index 23 (97% over 40bp)
GATCGGAAGAGACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGC20.1386001386001386TruSeq Adapter, Index 7 (95% over 24bp)
GTGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA20.1386001386001386TruSeq Adapter, Index 2 (97% over 36bp)
GTTCAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCT20.1386001386001386TruSeq Adapter, Index 23 (97% over 40bp)
GATCGGAAGAGCACACATCTGAACTCCAGTCACCAACTCTATCTCGTATG20.1386001386001386TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)