FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n01_HRBB2EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n01_HRBB2EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11333
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCTCGTATG8547.535515750463249TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCTCGTAT790.6970793258625253TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATATCGTATG360.3176564016588723TruSeq Adapter, Index 16 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCTCGTATGC140.12353304508956146TruSeq Adapter, Index 16 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)