FastQCFastQC Report
Tue 22 Dec 2020
000000000-JCJPN_l01_n01_HRBB1LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JCJPN_l01_n01_HRBB1LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG9475.2TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATTTCGTATG21.6TruSeq Adapter, Index 20 (97% over 38bp)
GTCTGAACTCCAGTCACGCTCCGTATCTCGTATGCCGTCTTCTGCTTGAA21.6RNA PCR Primer, Index 18 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTAT21.6TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCACATCTCGTATG21.6TruSeq Adapter, Index 20 (97% over 38bp)
ATTCACTGCAACATTTGATCCACACACAAAGAAACATCACGAGAGATCGG10.8No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGCATG10.8TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATACCTCGTATC10.8TruSeq Adapter, Index 20 (97% over 38bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATG10.8TruSeq Adapter, Index 21 (97% over 36bp)
TTCAGAATGAAGCGCGCCAGCGCCACTGTGCTGAGGAACCCGACAAGGCC10.8No Hit
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATCTCGTATGCC10.8TruSeq Adapter, Index 21 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCG10.8TruSeq Adapter, Index 20 (97% over 35bp)
GAGGCGATCTGATGATGTGCCAAGATCGGAAGAGCACACGTCTGAACTCC10.8Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCTGTCACGTTGCATATCTCGTATG10.8TruSeq Adapter, Index 21 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATATCGTATG10.8TruSeq Adapter, Index 20 (97% over 38bp)
AATAGCTATTTCGGTGTAAGCAAAGGTCTATTGTGCAATATCAAGTTGTT10.8No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTC10.8TruSeq Adapter, Index 20 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGCATATTTCGTATG10.8TruSeq Adapter, Index 20 (97% over 38bp)
GAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCGTCTTC10.8TruSeq Adapter, Index 20 (96% over 30bp)
TGTCTCTTTCACGTCTGAACTCCAGTCACGCTCCGTATCTCGTATGCCGT10.8TruSeq Adapter, Index 9 (95% over 24bp)
TATTTTAATTTAAAGATTCAATTTCAATGAATACAATGGTTTAGTCGGAT10.8No Hit
AGACACTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGC10.8TruSeq Adapter, Index 20 (97% over 38bp)
CACCTCCCCCTCGCGCCGCCGTCGCGTCACCGCCGAGTGCCCCCTCAGAT10.8No Hit
GGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTATGCCG10.8TruSeq Adapter, Index 21 (96% over 33bp)
GCGTGTTGTCTGAACTCCAGTCACGCTCCGTATCTCGTATGCCGTCTTCT10.8TruSeq Adapter, Index 18 (95% over 24bp)
CCCACATCCTGAACATCCGGATCATTGGTCACCATCCAGGGAATGGTTCA10.8No Hit
GATCGGAAGAGGCACACGTCTGAACTCCAGTCACGTTGCATATCTCGTAT10.8TruSeq Adapter, Index 20 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCATATCTCGTATG10.8TruSeq Adapter, Index 20 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)