FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504834
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTC2300.0110.3366855
TCTACGT3200.097.431234
CGTCTCG1800.096.675958
GTCTCGT3450.096.669529
CGGTCTC1200.084.591467
GGTCTCG1350.080.563298
AGTCTCG554.3930413E-879.09858
ACGTCTC2400.078.549217
TCCTCTA8400.078.546591
CTCGGTC1150.075.659445
CTCCGAC809.822543E-1172.5069665
CGACTCG1200.072.5069668
GTCTCGG851.6916601E-1068.237319
GGCTCGT754.8567017E-967.668679
TACGTCT2900.067.5064856
GTGAAGA8200.067.19487145
TGTCTCG2600.066.9295048
GACTCGT1009.094947E-1265.251939
ATCTCGT1350.064.446359
TGCCTCT7100.063.313831
Produced by FastQC (version 0.11.5)