FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222493
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT8020.36046077854134734No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG7640.3433815895331539No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAA7190.3231562341287142No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC7100.3191111630478262No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG6300.2831549756621557No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA5370.24135590782631364No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC5350.24045700314167187No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA5260.23641193206078392No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA5190.23326576566453774No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT4910.22068110007955308No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT4800.21573712431402337No Hit
GACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAG4700.21124260089081456No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG4290.1928150548556584No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC4040.18157874629763635No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA4020.18067984161299458No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC3810.17124134242425604No Hit
ATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCT3700.16629736665872633No Hit
CATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTA3620.1627017479201593No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA3610.1622522955778384No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG3490.1568588674699878No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT3470.15595996278534605No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG3410.15326324873142075No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA3400.1528137963890999No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA3380.15191489170445813No Hit
CTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTC3350.15056653467749548No Hit
GTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAA3160.1420269401733987No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTCACCTTTTAC3110.13977967846179432No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT3000.1348357026962646No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC2930.13168953630001845No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG2860.12854336990377224No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG2850.12809391756145136No Hit
GGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGA2820.12674556053448874No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG2790.12539720350752606No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC2730.12270048945360078No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC2710.12180158476895903No Hit
AGTCAGCACTGCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAA2670.1200037753996755No Hit
CGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTG2650.11910487071503373No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG2630.11820596603039196No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG2620.11775651368807108No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC2620.11775651368807108No Hit
GTGTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTA2580.11595870431878755No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA2550.11461034729182491No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA2470.11101472855325786No Hit
GTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGT2460.11056527621093697No Hit
CCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTA2420.10876746684165345No Hit
AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC2410.10831801449933257No Hit
GTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCG2390.10741910981469081No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT2390.10741910981469081No Hit
ATTATACAGTTTCCTGTTCACCTTTTACAATTAATTGCCAGGAACCTAAA2370.10652020513004905No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG2370.10652020513004905No Hit
ACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTA2360.10607075278772815No Hit
GCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAATCTGCAGCAG2330.1047223957607655No Hit
ATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCAC2290.10292458639148197No Hit
GTTTCAAGAGTGCGGGAGAAAATTGATCGTACAACACGAGCAGCCTCTGA2260.10157622936451934No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT2260.10157622936451934No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC2240.10067732467987757No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC2240.10067732467987757No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT2240.10067732467987757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAG351.895387E-9124.285712
TCTAACC203.8620635E-4108.753
CCCTAGC900.088.611111
ATAACCT259.377088E-487.03
TATAACC259.377088E-487.02
TAATAGG502.3003413E-672.53
ATCTAAC300.001933757172.52
TCCTGAC300.001933757172.5145
CTAATTT406.651813E-572.5145
TATGATC754.831236E-967.6666649
TTGTTCA1250.063.8000039
ATAGGCG606.7882684E-660.4166685
GCGTGTG606.7882684E-660.4166689
GGCGTGT651.0908669E-555.7692348
CTTGTAC400.006044727854.3751
CCTAATT400.006044727854.3752
CTGCGTG400.006044727854.3759
ATTAGCA701.6916883E-551.7857134
GTAGGCC701.6916883E-551.7857139
ATGTAGG701.6916883E-551.7857137
Produced by FastQC (version 0.11.5)