FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76064
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGATGGTGTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCT6130.8059002944888516No Hit
GTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTG3410.4483066891039125No Hit
TCTTTAGGCAGGTCCTTGATGTCACAGCGTCCTAGATGGTGTCCAGCAAT3270.42990113588557005No Hit
GTTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGC2680.352334875893984No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA2590.34050273453933527No Hit
CTCAAGGGCTGTTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTA2420.3181531342027766No Hit
GTCTCAAGGGCTGTTGTTTACAGAATAAATTGGATCACCGGTGGAATTGC2330.30632099284812786No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA2310.3036916281026504No Hit
CTGTTTATGAAAAACTCAAACCCGTCCTTTACCTTCCCAGGTAACAAACC2090.274768615902398No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA2010.264251156920488No Hit
GGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCA1960.2576777450567943No Hit
GGTCAGAATAGTGCCATGGAGTGGCACGTTGAGAAGAATGTTAGTTTCTG1900.2497896508203618No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG1760.23138409760201936No Hit
GAGTAAGACTACTGATGTGCACTTTACATCTGACATTTTAGACTGTACAG1640.21560790912915437No Hit
GGATCTGAAAACTTTGTCAGGGTAATAAACACCACGTGTGAAAGAATTAG1620.2129785443836769No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT1620.2129785443836769No Hit
GAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTA1610.21166386201093815No Hit
GCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTT1580.20771981489272193No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG1500.19720235591081195No Hit
TTCTAGAAGCGGTCTGGTCAGAATAGTGCCATGGAGTGGCACGTTGAGAA1480.19457299116533447No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT1460.19194362641985696No Hit
CTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTT1450.19062894404711822No Hit
GGGCTGTTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCA1420.186684896928902No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC1380.18142616743794698No Hit
GTCCACGAAGGATCACAGCTCCGATTACGAGTTCACTTTCTAGAAGCGGT1370.18011148506520824No Hit
GCACCCTGCATGGAAAGCAAAACAGAAAGTAGTGAAACCATTTTTTCAAA1360.1787968026924695No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1250.16433529659234328No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA1230.1617059318468658No Hit
CTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA1220.16039124947412706No Hit
ATGTCACAGCGTCCTAGATGGTGTCCAGCAATACGAAGATGTCCACGAAG1220.16039124947412706No Hit
GAATAGTGCCATGGAGTGGCACGTTGAGAAGAATGTTAGTTTCTGGATTG1160.15250315523769456No Hit
GTGTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCTCCGATTACGA1150.15118847286495582No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC1140.14987379049221708No Hit
GTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGAC1130.14855910811947834No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA1070.14067101388304587No Hit
GTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGA1060.1393563315103071No Hit
ATACGAAGATGTCCACGAAGGATCACAGCTCCGATTACGAGTTCACTTTC1060.1393563315103071No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA1060.1393563315103071No Hit
GTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGAC1050.13804164913756836No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA1020.13409760201935214No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC1010.13278291964661337No Hit
GTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTCCT1000.13146823727387463No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG990.1301535549011359No Hit
ATGTAGCAACAGTGATTTCTTTAGGCAGGTCCTTGATGTCACAGCGTCCT980.12883887252839715No Hit
GGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTG960.12620950778291964No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG960.12620950778291964No Hit
GTAGCAACAGTGATTTCTTTAGGCAGGTCCTTGATGTCACAGCGTCCTAG940.12358014303744215No Hit
CTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGA930.12226546066470341No Hit
GTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTC930.12226546066470341No Hit
AAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAAAAAT920.12095077829196467No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC920.12095077829196467No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT900.11832141354648716No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC890.11700673117374842No Hit
GTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTG890.11700673117374842No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG880.11569204880100968No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT880.11569204880100968No Hit
GTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTT870.11437736642827091No Hit
GTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCTCCGATTACGAGT860.11306268405553217No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC850.11174800168279343No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT840.11043331931005469No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC840.11043331931005469No Hit
GGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGA840.11043331931005469No Hit
CTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACT830.10911863693731595No Hit
GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGA830.10911863693731595No Hit
GCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC830.10911863693731595No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG820.10780395456457718No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA820.10780395456457718No Hit
ACATAACACAGTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATA810.10648927219183844No Hit
ACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAAC810.10648927219183844No Hit
CAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATA810.10648927219183844No Hit
AGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATG800.1051745898190997No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC790.10385990744636096No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT790.10385990744636096No Hit
TCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATG780.10254522507362222No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT770.10123054270088346No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA770.10123054270088346No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT770.10123054270088346No Hit
CCTAGATGGTGTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCTCC770.10123054270088346No Hit
GTGTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAA770.10123054270088346No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC770.10123054270088346No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG770.10123054270088346No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA770.10123054270088346No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT770.10123054270088346No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGAT100.0070780776145.0145
GTATCAG100.0070780776145.01
AAGTAAA203.8521865E-4108.75145
GTCTCAA404.7548383E-9108.751
CAGCATC259.3531946E-487.06
AGCATCT259.3531946E-487.07
TCTCAAG502.2404492E-887.02
ATAGGCG353.416853E-582.857145
GTAGGCC353.416853E-582.857149
ATGTAGG353.416853E-582.857147
GGCGTGT406.623711E-572.58
AGGCGTG406.623711E-572.57
GCGTGTG406.623711E-572.59
CCCTTTA300.001928848472.51
CCCTTAT300.001928848472.51
TAGGCGT406.623711E-572.56
TAATAGG451.18680546E-464.444443
AATGTAG451.18680546E-464.444446
AAGGGCT702.3057146E-762.1428574
CCTTATA350.003553848262.1428572
Produced by FastQC (version 0.11.5)