FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152487
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAA18281.1987907165856762No Hit
CTATTAAACAGCCTGCACGTGTTTGAAAAACATTAGAACCTGTAGAATAA11820.7751480454071494No Hit
CCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATA11100.7279309055853942No Hit
AATGTACATTGTACAATCTACTGATGTCTTGGTCATAGACACTGGTAGAA10990.7207171758904037No Hit
CATATGAGTTGTTGACATGTTCAGCCCCTATTAAACAGCCTGCACGTGTT10740.7043223356745165No Hit
GTCATAGACACTGGTAGAATTTCTGTGGTAACACTAATAGTAAAATTTGT9460.6203807537691738No Hit
GTACAATCTACTGATGTCTTGGTCATAGACACTGGTAGAATTTCTGTGGT9380.6151344049000899No Hit
GTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTT9040.5928374222064832No Hit
GTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGT8980.5889026605546702No Hit
ATCTACTGATGTCTTGGTCATAGACACTGGTAGAATTTCTGTGGTAACAC8760.5744752011646894No Hit
GAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGT8730.572507820338783No Hit
GTCTACAGCATCTGTAATGGTTCCATTTTCATTATATTTTAATAGAAAAG7490.4911894128679822No Hit
CAAATGTACATTGTACAATCTACTGATGTCTTGGTCATAGACACTGGTAG7370.48331988956435634No Hit
ATATAGGACAGAATAATCAGCAACACAGTTGCTGATTCTCTTCCTGTTCC7290.47807354069527236No Hit
CAGTAAGAACACCTGTGCCTGTTAAACCATTGAAGTTGAAATTGACACAT6850.44921862191531087No Hit
ACATTGTACAATCTACTGATGTCTTGGTCATAGACACTGGTAGAATTTCT6790.4452838602634979No Hit
TTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATG6590.43216798809078805No Hit
ACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACA6440.42233108396125574No Hit
CTCCATAACACTTAAAAGTGGAAAATGATGCGGAATTATATAGGACAGAA6310.41380576704899436No Hit
GCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACC6270.41118259261445234No Hit
GCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAAT6010.3941319587899296No Hit
GTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTAT5980.39216457796402315No Hit
GTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTA5960.3908529907467522No Hit
TTGTACAATCTACTGATGTCTTGGTCATAGACACTGGTAGAATTTCTGTG5950.39019719713811674No Hit
ACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAA5840.3829834674431263No Hit
GAATTAGTCTGAGTCTGATAACTAGCGCATATACCTGCACCAATGGGTAT5840.3829834674431263No Hit
ATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAG5820.3816718802258553No Hit
GTTAACATCCTGATAAAGAACAGCAACCTGGTTAGAAGTATTTGTTCCTG5770.3783929121826779No Hit
CATCAGTAGTGTCAGCAATGTCTCTGCCAAATTGTTGGAAAGGCAGAAAC5760.37773711857404235No Hit
CCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATA5650.370523388879052No Hit
CTACTACTCTGTATGGTTGGTAACCAACACCATTAGTGGGTTGGAAACCA5640.3698675952704165No Hit
GTGTAATGTCAAGAATCTCAAGTGTCTGTGGATCACGGACAGCATCAGTA5340.3501937870113518No Hit
GTAAGAACACCTGTGCCTGTTAAACCATTGAAGTTGAAATTGACACATTT5040.33051997875228706No Hit
TACCTGCACCAATGGGTATGTCACACTCATATGAGTTGTTGACATGTTCA5020.3292083915350161No Hit
CTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACA4970.3259294234918387No Hit
GTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAA4830.31674831297094175No Hit
GTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGG4780.31346934492776435No Hit
GTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTC4710.3088787896673159No Hit
GAATTATATAGGACAGAATAATCAGCAACACAGTTGCTGATTCTCTTCCT4690.30756720245004493No Hit
GCATAGACATTAGTAAAGCAGAGATCATTTAATTTAGTAGGAGACACTCC4630.303632440798232No Hit
ATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTA4630.303632440798232No Hit
ATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAG4620.3029766471895965No Hit
GTGTAGGCAATGATGGATTGACTAGCTACACTACGTGCCCGCCGAGGAGA4600.3016650599723255No Hit
GTTATAACACTGACACCACCAAAAGAACATGGTGTAATGTCAAGAATCTC4500.2951071238859706No Hit
CTACTGATGTCTTGGTCATAGACACTGGTAGAATTTCTGTGGTAACACTA4480.2937955366686996No Hit
TTGTTAGACTCAGTAAGAACACCTGTGCCTGTTAAACCATTGAAGTTGAA4310.2826470453218963No Hit
GGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACA4150.2721543475837284No Hit
GTTCAGCCCCTATTAAACAGCCTGCACGTGTTTGAAAAACATTAGAACCT3970.26035006262828964No Hit
GTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGG3920.2570710945851122No Hit
GATTATTCTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTA3890.2551037137592057No Hit
GTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTC3840.25182474571602825No Hit
GGGTATGTCACACTCATATGAGTTGTTGACATGTTCAGCCCCTATTAAAC3730.24461101602103785No Hit
GTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCC3710.24329942880376687No Hit
CCACAAATGTACATTGTACAATCTACTGATGTCTTGGTCATAGACACTGG3700.24264363519513138No Hit
GTACTACTACTCTGTATGGTTGGTAACCAACACCATTAGTGGGTTGGAAA3700.24264363519513138No Hit
ATTACAAGGTGTGCTACCGGCCTGATAGATTTCAGTTGAAATATCTCTCT3670.2406762543692249No Hit
ATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTG3570.23411831828287002No Hit
CAGTTGAATCACCACAAATGTACATTGTACAATCTACTGATGTCTTGGTC3570.23411831828287002No Hit
CACTAATAGTAAAATTTGTGGGTATGGCAATAGAGTTATTAGAGTAAGCA3530.23149514384832806No Hit
ATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTA3470.22756038219651512No Hit
GTGTTATAACACTGACACCACCAAAAGAACATGGTGTAATGTCAAGAATC3430.22493720776197312No Hit
GTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT3410.22362562054470217No Hit
CTGTATGGTTGGTAACCAACACCATTAGTGGGTTGGAAACCATATGATTG3350.21969085889288922No Hit
GTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAA3330.21837927167561827No Hit
TAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAG3320.21772347806698275No Hit
GTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCA3260.2137887164151698No Hit
CTCATATGAGTTGTTGACATGTTCAGCCCCTATTAAACAGCCTGCACGTG3210.21050974837199238No Hit
GCATCAGTAGTGTCAGCAATGTCTCTGCCAAATTGTTGGAAAGGCAGAAA3150.20657498672017943No Hit
TACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGA3140.2059191931115439No Hit
TTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGA3020.19804966980791805No Hit
CCATAACACTTAAAAGTGGAAAATGATGCGGAATTATATAGGACAGAATA3010.1973938761992826No Hit
ACTCTGTATGGTTGGTAACCAACACCATTAGTGGGTTGGAAACCATATGA3000.19673808259064707No Hit
ATTAAACAGCCTGCACGTGTTTGAAAAACATTAGAACCTGTAGAATAAAC2980.1954264953733761No Hit
CGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCA2980.1954264953733761No Hit
GTATGGTTGGTAACCAACACCATTAGTGGGTTGGAAACCATATGATTGTA2950.1934591145474696No Hit
GTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAA2930.19214752733019866No Hit
CACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTG2930.19214752733019866No Hit
ATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTG2880.18886855928702118No Hit
TTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTT2880.18886855928702118No Hit
GGTTAGAAGTATTTGTTCCTGGTGTTATAACACTGACACCACCAAAAGAA2840.18624538485247924No Hit
CTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAG2800.18362221041793725No Hit
GTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTC2770.18165482959203078No Hit
ATGTAGAAGTTCAAAAGAAAGTACTACTACTCTGTATGGTTGGTAACCAA2760.1809990359833953No Hit
TGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCG2750.18034324237475982No Hit
ATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAG2730.17903165515748884No Hit
GCATTCAGTTGAATCACCACAAATGTACATTGTACAATCTACTGATGTCT2710.17772006794021786No Hit
ATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAG2670.1750968935056759No Hit
ATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGC2640.17312951267976942No Hit
CTATAACGCAGCCTGTAAAATCATCTGGTAATTTATAATTATAATCAGCA2630.17247371907113393No Hit
ATTCAGTTGAATCACCACAAATGTACATTGTACAATCTACTGATGTCTTG2580.16919475102795647No Hit
ACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCT2520.16525998937614353No Hit
TGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATA2520.16525998937614353No Hit
AAGTAGGAGTAAGTTGATCTGCATGAATAGCAACAGGGACTTCTGTGCAG2510.16460419576750804No Hit
GGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGC2490.16329260855023706No Hit
GACATTAGTAAAGCAGAGATCATTTAATTTAGTAGGAGACACTCCATAAC2450.16066943411569512No Hit
GATAACTAGCGCATATACCTGCACCAATGGGTATGTCACACTCATATGAG2430.1593578468984241No Hit
CTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTT2420.15870205328978865No Hit
CAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGC2420.15870205328978865No Hit
AGATTGCTGCATTCAGTTGAATCACCACAAATGTACATTGTACAATCTAC2410.15804625968115316No Hit
CCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACC2380.15607887885524668No Hit
CATTAGAACCTGTAGAATAAACACGCCAAGTAGGAGTAAGTTGATCTGCA2360.1547672916379757No Hit
CAACAAAAGATTGCTGCATTCAGTTGAATCACCACAAATGTACATTGTAC2350.1541114980293402No Hit
CCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAG2310.15148832359479825No Hit
CCATTACAAGGTGTGCTACCGGCCTGATAGATTTCAGTTGAAATATCTCT2300.15083252998616276No Hit
CCATATGATTGTAAAGGAAAGTAACAATTAAAACCTTCAACACCATTACA2300.15083252998616276No Hit
CTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATT2290.15017673637752726No Hit
GCATATACCTGCACCAATGGGTATGTCACACTCATATGAGTTGTTGACAT2240.1468977683343498No Hit
CCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACC2230.14624197472571432No Hit
GGTCATAGACACTGGTAGAATTTCTGTGGTAACACTAATAGTAAAATTTG2230.14624197472571432No Hit
CTTCTGTGCAGTTAACATCCTGATAAAGAACAGCAACCTGGTTAGAAGTA2230.14624197472571432No Hit
GCAATAGAGTTATTAGAGTAAGCAACTGAATTTTCTGCACCAAGTGACAT2230.14624197472571432No Hit
GTAGGAGACACTCCATAACACTTAAAAGTGGAAAATGATGCGGAATTATA2190.14361880029117235No Hit
GTTGGTAACCAACACCATTAGTGGGTTGGAAACCATATGATTGTAAAGGA2180.14296300668253686No Hit
TTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAAT2170.14230721307390137No Hit
CTTTTGAATATGTCTCTCAGCCTTAATCCATCATTGCCTACACTATGTCA2150.1409956258566304No Hit
CATATACCTGCACCAATGGGTATGTCACACTCATATGAGTTGTTGACATG2110.13837245142208845No Hit
CCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACC2110.13837245142208845No Hit
GGAGTAAGTTGATCTGCATGAATAGCAACAGGGACTTCTGTGCAGTTAAC2090.13706086420481745No Hit
GTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTT2090.13706086420481745No Hit
ATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAA2080.13640507059618198No Hit
GTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGC2080.13640507059618198No Hit
ACTGATGTCTTGGTCATAGACACTGGTAGAATTTCTGTGGTAACACTAAT2070.1357492769875465No Hit
ATGTCACACTCATATGAGTTGTTGACATGTTCAGCCCCTATTAAACAGCC2060.13509348337891097No Hit
ACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTAT2050.1344376897702755No Hit
GTAAAGCAGAGATCATTTAATTTAGTAGGAGACACTCCATAACACTTAAA2030.1331261025530045No Hit
CCATTAGTGGGTTGGAAACCATATGATTGTAAAGGAAAGTAACAATTAAA2030.1331261025530045No Hit
GCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAA2020.13247030894436904No Hit
GTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTA2020.13247030894436904No Hit
TCAGTAGTGTCAGCAATGTCTCTGCCAAATTGTTGGAAAGGCAGAAACTT2010.13181451533573355No Hit
ATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTT2000.13115872172709805No Hit
GGTAGAATTTCTGTGGTAACACTAATAGTAAAATTTGTGGGTATGGCAAT2000.13115872172709805No Hit
GTGTGCTACCGGCCTGATAGATTTCAGTTGAAATATCTCTCTCAAAAGGT1960.1285355472925561No Hit
GGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTA1960.1285355472925561No Hit
GAATAATCAGCAACACAGTTGCTGATTCTCTTCCTGTTCCAAGCATAAAC1960.1285355472925561No Hit
CATTAGTAAAGCAGAGATCATTTAATTTAGTAGGAGACACTCCATAACAC1930.12656816646664962No Hit
GCCTGCACGTGTTTGAAAAACATTAGAACCTGTAGAATAAACACGCCAAG1920.12591237285801413No Hit
CCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAAC1920.12591237285801413No Hit
GTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTAT1890.12394499203210767No Hit
CTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCT1880.12328919842347216No Hit
CTTCTAACCAGGTTGCTGTTCTTTATCAGGATGTTAACTGCACAGAAGTC1880.12328919842347216No Hit
GTCACACTCATATGAGTTGTTGACATGTTCAGCCCCTATTAAACAGCCTG1870.12263340481483667No Hit
ACAAAAGATTGCTGCATTCAGTTGAATCACCACAAATGTACATTGTACAA1870.12263340481483667No Hit
CTATACAGGTAATTATAATTACCACCAACCTTAGAATCAAGATTGTTAGA1860.1219776112062012No Hit
GTATTTGTTCCTGGTGTTATAACACTGACACCACCAAAAGAACATGGTGT1850.12132181759756569No Hit
CTGATAACTAGCGCATATACCTGCACCAATGGGTATGTCACACTCATATG1850.12132181759756569No Hit
CTGTTAAACCATTGAAGTTGAAATTGACACATTTGTTTTTAACCAAATTA1840.1206660239889302No Hit
GTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATG1830.12001023038029472No Hit
GTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCAACAGA1790.11738705594575274No Hit
GAACACCTGTGCCTGTTAAACCATTGAAGTTGAAATTGACACATTTGTTT1780.11673126233711725No Hit
CACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTT1780.11673126233711725No Hit
GGTAATTATAATTACCACCAACCTTAGAATCAAGATTGTTAGAATTCCAA1730.1134522942939398No Hit
CTGGTAGAATTTCTGTGGTAACACTAATAGTAAAATTTGTGGGTATGGCA1700.11148491346803335No Hit
AAGTTGATCTGCATGAATAGCAACAGGGACTTCTGTGCAGTTAACATCCT1690.11082911985939785No Hit
GTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTG1680.11017332625076236No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA1660.10886173903349138No Hit
GAGTTATTAGAGTAAGCAACTGAATTTTCTGCACCAAGTGACATAGTGTA1650.1082059454248559No Hit
AAGTGACATAGTGTAGGCAATGATGGATTGACTAGCTACACTACGTGCCC1630.1068943582075849No Hit
CTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAA1630.1068943582075849No Hit
GTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTA1620.10623856459894943No Hit
TCTGTGCAGTTAACATCCTGATAAAGAACAGCAACCTGGTTAGAAGTATT1610.10558277099031393No Hit
ATTATATAGGACAGAATAATCAGCAACACAGTTGCTGATTCTCTTCCTGT1590.10427118377304295No Hit
AAGAACACCTGTGCCTGTTAAACCATTGAAGTTGAAATTGACACATTTGT1590.10427118377304295No Hit
CATTTGTTTTTAACCAAATTAGTAGACTTTTTAGGTCCACAAACAGTTGC1560.10230380294713648No Hit
CCAATGGGTATGTCACACTCATATGAGTTGTTGACATGTTCAGCCCCTAT1540.1009922157298655No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGC100.00708501145.03
TCGCGAA100.00708501145.0145
TACACTG100.00708501145.07
GCATTAT100.00708501145.01
GTTATGC100.00708501145.03
CATACTC203.8597055E-4108.753
TCATACT203.8597055E-4108.752
GGGTAGC259.3713833E-487.01
GTCTCAC259.3713833E-487.0145
GGTAGCC259.3713833E-487.02
TACTCAG259.3713833E-487.05
TAGCCAT300.001932585672.54
CCATCAG300.001932585672.57
ATCAGGG350.00356070962.1428579
CCTTCCC350.00356070962.1428578
ACCTTCC350.00356070962.1428577
TGTAGAC4350.060.0000043
ACTGTGC4500.058.0000048
AAAAGAT753.7399514E-758.0000043
GCTGTAG4500.058.0000041
Produced by FastQC (version 0.11.5)