FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235610
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGAAATCAAT32921.397224226475956No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT30081.2766860489792453No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC18630.7907134671703238No Hit
GTAATAAGAACACCATTACGGGCATTTCTAAATAAGTCTACTTGACCATC16480.6994609736428844No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA14310.6073596197105386No Hit
GTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCC13130.5572768558210602No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG12440.527991171851789No Hit
GTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTA12070.5122872543610203No Hit
GTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGA11350.4817282797843894No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA11140.47281524553287213No Hit
CGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGC11080.4702686643181529No Hit
GATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAA10350.43928525953906883No Hit
GTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTATAATAATTGAA9620.4083018547599847No Hit
CTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTG9500.40320869233054624No Hit
GTGTTATACACGTAGCAGACTTTAGTGGTACATAATCTATATCTGACACT8860.37604515937354105No Hit
GTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAAT8130.3450617545944569No Hit
ATCTATATCTGACACTACTTGTTTTCCATGAGACTCACATGGACTGTCAG8110.34421289418955053No Hit
GGTTATAAGTATCAAATTGTTTGTAAACCCACAAGCTAAAGCCAGCTGAG7990.33911973176011206No Hit
GAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATT7950.3374220109502992No Hit
GCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATG7870.33402656933067354No Hit
GTTATGAAATAGTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAA7730.3280845464963287No Hit
TTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGC7520.31917151224481133No Hit
GTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAAT7280.3089851873859344No Hit
ATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCC7110.3017698739442299No Hit
TTGTTGGACAACACCATCAACTTTCTTATAATAATTGAACTGTGTTTTTA7030.29837443232460426No Hit
GATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTG6990.2966767115147914No Hit
ACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATG6980.2962522813123382No Hit
TTTCTACTCTGAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATC6910.29328126989516573No Hit
GTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAA6750.2864903866559145No Hit
GTGTTATTAATGATAGAAACTGGTACTTCACCCTGTTGTCCATCAAAGTG6710.2847926658461016No Hit
GTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCA6630.28139722422647595No Hit
ATTCTACATCAACACCATCAACTTTTGTGTAAACAGTGTTATTAATGATA6610.2805483638215695No Hit
GTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAAT6310.2678154577479734No Hit
GGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAATT6290.26696659734306694No Hit
ATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCA6110.25932685369890923No Hit
CTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGC5980.25380926106701757No Hit
CCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCATTTCTAAA5980.25380926106701757No Hit
GTTATACACGTAGCAGACTTTAGTGGTACATAATCTATATCTGACACTAC5930.25168711005475153No Hit
GAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGT5900.2504138194473919No Hit
GTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTA5580.23683205296888926No Hit
GTTGTAAACCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCA5490.2330121811468104No Hit
TATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAA5440.23089003013454437No Hit
GGTTTAATGTTGCGCTTAGCCCAAAGCTCAAATGCTACATTAACAGGTAA5380.22834344891982514No Hit
CTACAAGGCTGTGCATCATAGAACTTCCATTCTACATCAGCTTGAGGTAC5330.2262212979075591No Hit
ATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGTT5320.22579686770510587No Hit
GGCATACACCATCTGTGAATTTGTCAGAATGTGTGGCATAAGAATAGAAT5260.22325028649038667No Hit
GAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTA5220.22155256568057383No Hit
CATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACC5170.2194304146683078No Hit
CGTTTAGCTAGTCCAATCAGTAGATGTAAACCACCTAACTGACTATGACT5150.2185815542634014No Hit
CACATGGACTGTCAGAGTAATAGAAAAATGGTAATTGTTTTAAATTAACA5070.2151861126437757No Hit
GTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACA5040.2139128220364161No Hit
CAGTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGA4870.20669750859471162No Hit
GTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGT4520.19184245150884938No Hit
GCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGC4490.19056916090148976No Hit
GCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACA4450.18887144009167692No Hit
GTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCA4430.18802257968677052No Hit
GCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATC4400.1867492890794109No Hit
TCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCA4370.18547599847205126No Hit
GTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGT4360.18505156826959807No Hit
GTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAA4350.18462713806714487No Hit
CCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCT4290.1820805568524256No Hit
CCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGAC4260.18080726624506602No Hit
GATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGT4250.1803828360426128No Hit
GGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAG4190.17783625482789356No Hit
GGGTTTAAATTCTTGTAAATTTCTACTCTGAGTAAAGTAAGTTTCAGGTA4130.17528967361317432No Hit
GTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA4110.1744408132082679No Hit
AATTCTAAGAAATCAATTTCCATTTGACTCCTGGGTTTAAATTCTTGTAA4110.1744408132082679No Hit
CATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACA4100.17401638300581468No Hit
CTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAAT4090.17359195280336148No Hit
AGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAA4010.17019651118373583No Hit
GTATTAGCAGCAATGTCCACACCCAAATTATTGAGTATTTTCACCTCTGG3940.1672254997665634No Hit
ATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAG3940.1672254997665634No Hit
GTGTAAACAGTGTTATTAATGATAGAAACTGGTACTTCACCCTGTTGTCC3940.1672254997665634No Hit
ATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAA3930.16680106956411017No Hit
CCATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAAC3840.16298119774203132No Hit
CTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACA3840.16298119774203132No Hit
AGTCCAATCAGTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCC3830.16255676753957812No Hit
CAGTATTAGCAGCAATGTCCACACCCAAATTATTGAGTATTTTCACCTCT3830.16255676753957812No Hit
GTTATAAGCATCGAGATACAATCTGTACTCATTAGCATGATGTCTACAGA3800.1612834769322185No Hit
AGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACACTACC3690.1566147447052332No Hit
TAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTG3620.15364373328806077No Hit
ACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGAC3610.15321930308560758No Hit
CACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATC3570.15152158227579474No Hit
GTTTAAATTCTTGTAAATTTCTACTCTGAGTAAAGTAAGTTTCAGGTAAT3550.15067272187088832No Hit
CTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCG3540.15024829166843515No Hit
CATTAGCATGATGTCTACAGACAGCACCACCTAAATTGCAACGTGTTATA3520.1493994312635287No Hit
GCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGT3480.14770171045371588No Hit
CCTGTTGTCTATGTGATAAGAAAGTTGATGGTGTTGTCCAACAATTACCT3470.1472772802512627No Hit
ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAG3450.14642841984635627No Hit
ACATTAACAGGTAATGTTGTTTTATTTTCAAACAATTCTACATCAACACC3430.14557955944144985No Hit
AATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGG3430.14557955944144985No Hit
ATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATA3370.1430329782267306No Hit
ACCTAACTGACTATGACTAAAATCTCCATAAACGATATGTTCGAAGGCAT3360.14260854802427742No Hit
GCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGA3330.14133525741691777No Hit
AGATATATGTGCTGGAGCATCTCTTTTGTAGTCCCAGATCACAGTATTAG3330.14133525741691777No Hit
CTTTAGTGGTACATAATCTATATCTGACACTACTTGTTTTCCATGAGACT3320.14091082721446457No Hit
GTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCT3310.14048639701201138No Hit
CTTAATAGCTTTAGGGTTACCAATGTCGTGAAGAACTGGGAATTTGTCTG3300.14006196680955818No Hit
GTAGATATATGTGCTGGAGCATCTCTTTTGTAGTCCCAGATCACAGTATT3270.13878867620219856No Hit
GTTCGAAGGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATA3200.1358176647850261No Hit
GTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTA3190.13539323458257288No Hit
GTCTACAGACAGCACCACCTAAATTGCAACGTGTTATACACGTAGCAGAC3190.13539323458257288No Hit
TAATTGAACTGTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAG3170.1345443741776665No Hit
ATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTT3160.13411994397521326No Hit
GACTATGACTAAAATCTCCATAAACGATATGTTCGAAGGCATAGCCTTCT3160.13411994397521326No Hit
AGGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAAT3140.13327108357030687No Hit
CCTTAAAACGTTTAGCTAGTCCAATCAGTAGATGTAAACCACCTAACTGA3110.13199779296294725No Hit
GAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGT3100.13157336276049403No Hit
CTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAA3090.13114893255804083No Hit
GTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA3090.13114893255804083No Hit
CATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTT3080.13072450235558764No Hit
GTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTG3050.129451211748228No Hit
TCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAAC3010.12775349093841518No Hit
GAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTG2990.12690463053350878No Hit
TCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTC2970.12605577012860233No Hit
CTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGAT2950.12520690972369594No Hit
GTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTG2930.12435804931878952No Hit
GTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATA2930.12435804931878952No Hit
CACCAATAGTAGATATATGTGCTGGAGCATCTCTTTTGTAGTCCCAGATC2890.12266032850897671No Hit
ATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAG2870.1218114681040703No Hit
TTTCTAAATAAGTCTACTTGACCATCAACTCTACCATCAAAAAAGACAGT2860.12138703790161708No Hit
CCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGTTCGAAGGC2850.12096260769916387No Hit
ATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACAC2840.12053817749671067No Hit
ATTTACAACATTAAAAGCCACATTTTCTAAACTCTGAAGTCTTGTAAAAG2840.12053817749671067No Hit
CTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTG2840.12053817749671067No Hit
CTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTA2810.11926488688935105No Hit
GTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAGACTAGCTTGT2790.11841602648444463No Hit
GCTTTAGGGTTACCAATGTCGTGAAGAACTGGGAATTTGTCTGCTAATAA2780.11799159628199142No Hit
CTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCC2770.11756716607953822No Hit
GAATAGAATAATTCTTCTATTTTATAAGCTTTGTCACTACAAGGCTGTGC2750.11671830567463182No Hit
GTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCA2720.11544501506727219No Hit
ATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTA2720.11544501506727219No Hit
CCATTACGGGCATTTCTAAATAAGTCTACTTGACCATCAACTCTACCATC2710.11502058486481898No Hit
ATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCT2690.11417172445991257No Hit
CCATCAAAGTGTCCCTTATTTACAACATTAAAAGCCACATTTTCTAAACT2680.11374729425745936No Hit
CTACAGATGGTTGTAAACCTTTAACACTACCTTCTGTAATAAGAACACCA2670.11332286405500616No Hit
GCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTA2570.10907856203047409No Hit
GTACATAATCTATATCTGACACTACTTGTTTTCCATGAGACTCACATGGA2520.10695641101820805No Hit
AGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGT2510.10653198081575486No Hit
GTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTAT2510.10653198081575486No Hit
GTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTA2510.10653198081575486No Hit
TCTATCGACATTGCAATTCCAAAATAGGCATACACCATCTGTGAATTTGT2460.10440982980348883No Hit
CTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGT2450.10398539960103562No Hit
GTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGT2440.10356096939858239No Hit
GAATTCATCCATAGCTAATTCTAAGAAATCAATTTCCATTTGACTCCTGG2440.10356096939858239No Hit
CATCAACACCATCAACTTTTGTGTAAACAGTGTTATTAATGATAGAAACT2400.10186324858876958No Hit
AGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTC2390.10143881838631638No Hit
GTTTGCGCATCTGTTATGAAATAGTTTTTAACTGTACTGTCCATAGGAAT2370.10058995798140995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGCC100.0070874477145.03
GCCTCCT100.0070874477145.01
GTACCGT100.0070874477145.01
CGCTCAA100.0070874477145.01
TGGATGC100.0070874477145.02
GGCGTGT203.86235E-4108.758
GCGTGTG203.86235E-4108.759
AGGCGTG300.001933899672.57
TGTCTAT2750.071.1818166
TCTATGT2500.066.78
TTGTCTA2850.066.140355
GTTGTCT2900.065.04
GGGTAGC350.00356312162.1428571
TAGCCAT350.00356312162.1428574
GGTAGCC350.00356312162.1428572
GTCTATG3000.060.4166687
CTATGTG2600.055.769239
GAGGCAC400.006045169754.3756
AGAGGCA400.006045169754.3755
CATCAGG400.006045169754.3758
Produced by FastQC (version 0.11.5)