FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265391
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC18720.7053743344725331No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT15170.5716094366425387No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC11230.42314924017770006No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG10320.3888602100297297No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA10220.38509218473874396No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT9990.37642572656947676No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC9950.37491851645308244No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC9750.3673824658711109No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG9640.3632376380510266No Hit
ACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGT8690.327441397786662No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG8510.3206589522628876No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC8150.3070940612153389No Hit
GTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAGC7900.2976739979878745No Hit
AGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATA7760.29239876258049446No Hit
GTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAA7740.2916451575222973No Hit
GTGTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTA7630.28750032970221295No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG7500.28260189682393144No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG7340.2765730563583543No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG7240.27280503106736853No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA6840.2577329299034255No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA6600.24868966920505972No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG6320.2381391983902996No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC6260.23587838321570812No Hit
GTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGT6140.23135675286652524No Hit
GTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAG6000.22608151745914518No Hit
GCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTG5960.2245743073427509No Hit
GTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGG5940.22382070228455375No Hit
CACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTA5830.2196758744644694No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA5820.21929907193537082No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG5810.21892226940627224No Hit
GTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCA5710.2151542441152865No Hit
GAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCG5650.21289342894069505No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA5560.20950220617880788No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA5540.20874860112061072No Hit
GTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCACATGT5440.20498057582962495No Hit
ACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAA5360.20196615559683637No Hit
CTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTT5100.19216928984027343No Hit
GTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATCAT5050.19028527719478053No Hit
GATTAACAGACAAGACTAATTTATGTGATGTTGATATGACATGGTCGTAA4950.18651725190379478No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC4840.18237242408371046No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT4780.18011160890911898No Hit
GTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTA4640.17483637350173895No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC4590.17295236085624605No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT4540.17106834821075317No Hit
ATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTC4530.17069154568165462No Hit
AAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTT4520.17031474315255604No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG4510.16993794062345746No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC4430.16692352039066888No Hit
GTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATC4400.16579311280337314No Hit
ACCTGGAGCATTGCAAACATACGGATTAACAGACAAGACTAATTTATGTG4390.16541631027427456No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA4360.16428590268697882No Hit
ATGGAAAACTAATGGGTGGTTTATGTGATTTACAATAATAGCTCATACCT4280.16127148245419023No Hit
GAGATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTG4220.15901066727959878No Hit
GGTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAG4210.1586338647505002No Hit
GCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGC4200.15825706222140162No Hit
GCATAACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGC4110.15486583945951446No Hit
GCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTAT4050.152605024284923No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT4040.15222822175582443No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG3990.15034420911033156No Hit
CTGTAAGACTGTATGCGGTGTGTACATAGCCTCATAAAACTCAGGTTCCC3970.1495906040521344No Hit
TTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGG3960.14921380152303582No Hit
CTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACA3950.14883699899393724No Hit
AATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAA3890.14657618381934578No Hit
CTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATT3870.14582257876114865No Hit
ATCATAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGC3810.1435617635865572No Hit
GTTGTACCTCGGTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGT3800.14318496105745862No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA3800.14318496105745862No Hit
CTTCACGTACAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTA3730.1405473433537686No Hit
ATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAA3690.1390401332373743No Hit
CTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTT3570.1345185028881914No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG3570.1345185028881914No Hit
TTTCAGTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCA3560.1341417003590928No Hit
GTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGT3560.1341417003590928No Hit
GTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCA3540.13338809530089565No Hit
GTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACA3530.13301129277179707No Hit
CAATAATAGCTCATACCTCCTAAGTAAAGTTGAGTCACATCTGTGACATC3480.1311272801263042No Hit
ACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCT3380.12735925483531846No Hit
CTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT3370.12698245230621988No Hit
GAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGG3360.12660564977712133No Hit
GTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAAC3330.1254752421898256No Hit
GGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGG3330.1254752421898256No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT3320.125098439660727No Hit
GTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCAC3320.125098439660727No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT3310.12472163713162844No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT3300.12434483460252986No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG3260.12283762448613555No Hit
ACATAAGAATGGTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTG3200.1205768093115441No Hit
TGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCAT3180.11982320425334694No Hit
TACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCA3180.11982320425334694No Hit
TGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGT3170.11944640172424838No Hit
GGTGTACTCTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAG3140.11831599413695264No Hit
GTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGC3130.11793919160785406No Hit
CAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGC3120.1175623890787555No Hit
TAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGCCCCT3100.11680878402055835No Hit
GCATACTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAA3090.11643198149145977No Hit
GTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTA3070.11567837643326262No Hit
ATGTAATTCTCTGTCAGACAGCACTTCACGTACAGTAGCAATACCATAAG3030.11417116631686833No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA3020.11379436378776975No Hit
CTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGT3010.11341756125867117No Hit
GTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGT3000.11304075872957259No Hit
CTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAA3000.11304075872957259No Hit
GTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTA2980.11228715367137546No Hit
GTTTTTAGTTACACGATAACCAGTAAAGACATAATTTCGGTTAAGTGGTG2970.11191035114227688No Hit
GCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCT2970.11191035114227688No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT2930.11040314102588257No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG2930.11040314102588257No Hit
CTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTA2920.11002633849678399No Hit
TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT2920.11002633849678399No Hit
TTTCAAAGGTGTACTCTCCTATTTGTACTTTACTGTTTTTAGTTACACGA2880.1085191283803897No Hit
ACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTAC2880.1085191283803897No Hit
AGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAA2850.10738872079309396No Hit
AAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAA2830.10663511573489681No Hit
ATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCA2810.10588151067669965No Hit
TCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAA2810.10588151067669965No Hit
TCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTATTTGTACTTTACT2780.10475110308940394No Hit
ACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTAT2760.10399749803120678No Hit
GCATTAAAGTCAGTAACATTATCGCTACCAACACATGTATTTTTATATAA2760.10399749803120678No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA2730.10286709044391106No Hit
GGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTAC2720.10249028791481248No Hit
GATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTG2720.10249028791481248No Hit
TTCTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTA2710.1021134853857139No Hit
GTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTA2700.10173668285661534No Hit
ACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGA2690.10135988032751676No Hit
CATTTAATTTGTAAGTTGTTGTACCTCGGTAAACAACAGCATCACCATAG2680.10098307779841818No Hit
GTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGTA2680.10098307779841818No Hit
GTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGG2670.1006062752693196No Hit
TGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCA2660.10022947274022102No Hit
GGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTC2660.10022947274022102No Hit
TTAGCACACAATGGAAAACTAATGGGTGGTTTATGTGATTTACAATAATA2660.10022947274022102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGC100.0070879473145.01
GCGTGTG306.493792E-10145.09
GCCCGAT100.0070879473145.01
GAAGGGT700.0134.642851
ATAGGCG351.897206E-9124.285715
GGCGTGT351.897206E-9124.285718
AGGCGTG351.897206E-9124.285717
TAGGCGT351.897206E-9124.285716
GATAGAG1000.0108.752
TAGAGCC1050.0103.571434
ATAGAGC1150.094.565223
AGAGCCA1150.094.565225
TAATAGG502.2622771E-887.03
GAGCCAT1300.083.653856
TGATAGA1350.080.555551
AGCCATG1400.077.678577
GCCATGC1450.075.000018
GGTAGTA2300.072.52
AATGTAG451.1922484E-464.444456
CCATGCC1700.063.9705859
Produced by FastQC (version 0.11.5)