FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194438
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGCACATTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAAC14670.7544821485512092No Hit
GATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAAC11970.6156204034190847No Hit
GCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACG8740.4495006120202841No Hit
GTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATT8600.44230037338380357No Hit
AACTTATACTTAGGTGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAG8000.411442207798887No Hit
GGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACACTGAAAAAGTC7510.3862413725712052No Hit
CTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGT6230.32041061932338327No Hit
GTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTCCT5710.2936668758164556No Hit
GATATGGAACGTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTC5440.2797807013032432No Hit
GGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGA5400.27772349026424875No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC5050.25972289367304746No Hit
CAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATG4990.2566370771145558No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA4930.25355126055606414No Hit
GTTTCAAGAGTGCGGGAGAAAATTGATCGTACAACACGAGCAGCCTCTGA4820.24789393019882944No Hit
TGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATT4820.24789393019882944No Hit
TTACCAGCCTGTACCAAGAAATTATGATTAGACTTACGAATGAGTAAATC4610.23709357224410868No Hit
GTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACACAATTTTGCAT4610.23709357224410868No Hit
CCATTGTAACAAGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAAC4510.2319505446466226No Hit
GTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATA4310.2216644894516504No Hit
AGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAACAAACTTATACT4280.2201215811724046No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT4220.2170357646139129No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA4170.21446425081516987No Hit
GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGA4060.2088069204579352No Hit
GTGTAGTTGTACCACAAGTTACTTGTACCATACAACCCTCAACTTTACCA4040.20777831493843796No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT3960.2036638928604491No Hit
GTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAG3950.20314959010070047No Hit
GTCTTGGACAGTAAACTACGTCATCAAGCCAAAGACCGTTAAGTGTAGTT3890.20006377354220883No Hit
AACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCT3880.19954947078246024No Hit
CTTATACTTAGGTGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTA3860.198520865262963No Hit
GTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGA3840.1974922597434658No Hit
ACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTA3700.19029202110698526No Hit
TTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACACTGAAAAAG3650.18772050730824222No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG3500.18000596591201307No Hit
ACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAA3470.17846305763276726No Hit
GTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACT3450.17743445211327002No Hit
CGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTT3420.17589154383402422No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA3390.1743486355547784No Hit
GTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAAC3340.17177712175603535No Hit
GTATCAACCTTAAGCTTAAGTACACAATTTTGCATAGAATGTCCAATAAC3330.17126281899628673No Hit
GTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTT3240.16663409415854927No Hit
CCAATATTCCAGGCACCTTTTTTAGCTTTTCCTTTTGTAACTTTAAAATT3210.1650911858793034No Hit
ACACCAGATGGTGAACCATTGTAACAAGCTAACACTGAAAAAGTCTGTCC3140.16149106656106318No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG3040.15634803896357707No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA2990.15377652516483403No Hit
ACCTTAAGCTTAAGTACACAATTTTGCATAGAATGTCCAATAACCCTGAG2950.1517193141258396No Hit
TGCATAAAGAGGACTCAGTATTGATTTCTGTTCACCAATATTCCAGGCAC2940.151205011366091No Hit
TCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATG2930.15069070860634237No Hit
GTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAA2930.15069070860634237No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA2890.14863349756734795No Hit
GTACACAATTTTGCATAGAATGTCCAATAACCCTGAGTTGAACATTACCA2880.14811919480759933No Hit
GTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGA2880.14811919480759933No Hit
TCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTC2860.14709058928810212No Hit
TCATAGCATCAATGAGTCTCAGTGAATACTGTGAAATTCCATCTAGTATT2820.14503337824910767No Hit
CAGTGAATACTGTGAAATTCCATCTAGTATTGTTATAGCGGCCTTCTGTA2780.14297616721011325No Hit
TGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAAC2730.1404046534113702No Hit
CTTCAGAGGTGCAGATCACATGTCTTGGACAGTAAACTACGTCATCAAGC2690.13834744237237576No Hit
GGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTG2660.13680453409312995No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC2650.13629023133338133No Hit
CACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATA2630.13526162581388412No Hit
TACTTGTACCATACAACCCTCAACTTTACCAGATGGGAATGCCATTTTTC2620.1347473230541355No Hit
GCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACA2620.1347473230541355No Hit
ACATAAGAGAACACACAGCCTCCAAAGGCAATAGTGCGACCACCCTTACG2610.1342330202943869No Hit
CTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGT2600.1337187175346383No Hit
ATTGTAACAAGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAACAA2580.13269011201514105No Hit
GCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGACATGAC2550.13114720373589525No Hit
ACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAA2540.13063290097614663No Hit
GTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTT2530.130118598216398No Hit
CTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTG2520.12960429545664942No Hit
GCACATTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACACTGA2510.1290899926969008No Hit
ATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATA2500.1285756899371522No Hit
ACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGT2500.1285756899371522No Hit
GTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTAT2500.1285756899371522No Hit
AGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATG2490.1280613871774036No Hit
GTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGT2420.12446126785916334No Hit
GATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGC2420.12446126785916334No Hit
ATACTTAGGTGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACAC2390.1229183595799175No Hit
CTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTT2380.1224040568201689No Hit
ACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAG2380.1224040568201689No Hit
GTTACAACCTATGTTAGCGCTAGCACGTGGAACCCAATAGGCACACTTGT2340.12034684578117445No Hit
TTATAATCCAAACCTTTCACAGTTTCCACAAAAGCACTTGTGGAAGCAGA2330.11983254302142586No Hit
GTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATC2320.11931824026167726No Hit
CCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGG2290.11777533198243141No Hit
GTAACAAGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAACAAACT2280.11726102922268282No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA2250.11571812094343698No Hit
GTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACG2240.11520381818368837No Hit
GACTAAACCATGTGTCAAAATCAGCATGTTTGTTAGCAAAACAAGTATCT2230.11468951542393978No Hit
GATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAA2220.11417521266419116No Hit
GAACAACACCACCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCC2210.11366090990444254No Hit
AGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTA2210.11366090990444254No Hit
GTGAAATTGGGCCTCATAGCACATTGGTAAACACCAGATGGTGAACCATT2190.11263230438494534No Hit
GTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTG2160.1110893961056995No Hit
GTCCTGGTTGAATGCGAACAAACTTATACTTAGGTGTCTTAGGATTGGCT2160.1110893961056995No Hit
GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC2150.11057509334595089No Hit
GGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGG2140.1100607905862023No Hit
GTACCATACAACCCTCAACTTTACCAGATGGGAATGCCATTTTTCTAAAA2140.1100607905862023No Hit
CACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAA2140.1100607905862023No Hit
ATTTTGCATAGAATGTCCAATAACCCTGAGTTGAACATTACCAGCCTGTA2130.10954648782645368No Hit
GGGTTAAGCATGTCTTCAGAGGTGCAGATCACATGTCTTGGACAGTAAAC2120.10903218506670506No Hit
CCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAAC2110.10851788230695646No Hit
GGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACC2100.10800357954720784No Hit
GAAAAAGTCTGTCCTGGTTGAATGCGAACAAACTTATACTTAGGTGTCTT2100.10800357954720784No Hit
CTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTA2100.10800357954720784No Hit
CATTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACACTGAAAA2100.10800357954720784No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC2090.10748927678745925No Hit
AATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTA2080.10697497402771064No Hit
CTTCATGAGAGTTGAAAGGCACATTTGGTTGCATTCATTTGGTGACGCAA2070.10646067126796202No Hit
TTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTC2070.10646067126796202No Hit
CATAGCACATTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACA2050.1054320657484648No Hit
GCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAA2030.1044034602289676No Hit
ATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTGAACAT2030.1044034602289676No Hit
CCTTAAGCTTAAGTACACAATTTTGCATAGAATGTCCAATAACCCTGAGT2020.10388915746921898No Hit
GACCGTTAAGTGTAGTTGTACCACAAGTTACTTGTACCATACAACCCTCA2020.10388915746921898No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT1990.10234624918997316No Hit
CAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGAC1980.10183194643022454No Hit
ATGGTGAACCATTGTAACAAGCTAACACTGAAAAAGTCTGTCCTGGTTGA1980.10183194643022454No Hit
CGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCAT1970.10131764367047594No Hit
CAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAG1950.10028903815097871No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC351.0913936E-11145.03
CCGTTCA100.0070865145.0145
TCTATTC100.0070865145.03
GGGAAAG100.0070865145.03
TGGGAAA100.0070865145.02
ACATTGC553.6379788E-12105.454551
GCTTTGA1750.091.142861
ATAGGCG502.259003E-887.05
TTGATAT1900.083.947374
TAATAGG554.375761E-879.090913
CATTGCT755.4569682E-1177.333332
TAGCACA3900.074.358984
AACGTTA3350.073.582098
AAATTAG1000.072.55
GCGTGTG607.997551E-872.59
GATATGG4550.071.70331
TATAAAT1050.069.0476152
CACATTG4300.067.441867
TGGAACG4800.064.9479145
ACGTTAA3800.064.868429
Produced by FastQC (version 0.11.5)