FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56560
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAACACCGTGTAACTATGTTAGTAGTTGTACTAACAACTTTGTTAA3990.7054455445544555No Hit
GGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTT2600.4596888260254597No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC2000.3536067892503536No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT1820.3217821782178218No Hit
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC1820.3217821782178218No Hit
CTCTTAACAGTATTCTTTGCTATAGTAGTCGGCATAGATGCTTTAATTCT1810.32001414427157No Hit
GAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACA1650.2917256011315417No Hit
CTATTAACAGCAGCTAAACCATGAGTAGCAAGGGTTTTCAAACCTAATAC1400.24752475247524752No Hit
CTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTT1300.22984441301272984No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA1300.22984441301272984No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA1230.21746817538896746No Hit
CTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAA1230.21746817538896746No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT1200.21216407355021216No Hit
GGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGA1130.19978783592644977No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT1070.1891796322489392No Hit
AGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATG1070.1891796322489392No Hit
CAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTG1070.1891796322489392No Hit
CCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATC1040.1838755304101839No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA1030.18210749646393212No Hit
GTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCA1030.18210749646393212No Hit
ACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGA1030.18210749646393212No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT1020.18033946251768035No Hit
CTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTT980.17326732673267328No Hit
GTCTTATTACAGTAGGCTAAGATAAGTGCACAAAAGTTAGCAGCTTCACC940.1661951909476662No Hit
GTGTAACTATGTTAGTAGTTGTACTAACAACTTTGTTAAGAAAAGGCTTA930.16442715700141441No Hit
ACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAA900.15912305516265912No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC890.15735502121640735No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG870.1538189533239038No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACT830.14674681753889673No Hit
GTTTCAAGAGTGCGGGAGAAAATTGATCGTACAACACGAGCAGCCTCTGA830.14674681753889673No Hit
GCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTT820.14497878359264496No Hit
CCGTGTAACTATGTTAGTAGTTGTACTAACAACTTTGTTAAGAAAAGGCT820.14497878359264496No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG810.14321074964639322No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT800.14144271570014144No Hit
GTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA790.13967468175388967No Hit
GTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTAT770.13613861386138612No Hit
GTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAAC770.13613861386138612No Hit
GCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTC740.13083451202263083No Hit
GTAATAAGCATCTTGTAGAGCAGGTGGATTAAACTTCAACTCTATTTGTT740.13083451202263083No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC740.13083451202263083No Hit
CCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAA720.1272984441301273No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG700.12376237623762376No Hit
GTACAAACACGGTTTAAACACCGTGTAACTATGTTAGTAGTTGTACTAAC700.12376237623762376No Hit
GCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAAC690.12199434229137199No Hit
GTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAG680.12022630834512021No Hit
TTGCTATAGTAGTCGGCATAGATGCTTTAATTCTAGAATTTGTACTTCTA680.12022630834512021No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA640.11315417256011315No Hit
GGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACC620.10961810466760963No Hit
GTGTATGCCCCTCCGTTAAGCTCACGCATGAGTTCACGGGTAACACCACT620.10961810466760963No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG620.10961810466760963No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC620.10961810466760963No Hit
GTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTCCT620.10961810466760963No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG610.10785007072135785No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC610.10785007072135785No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG610.10785007072135785No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA610.10785007072135785No Hit
GAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGC610.10785007072135785No Hit
GTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGAC600.10608203677510608No Hit
CATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAG600.10608203677510608No Hit
TCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATG600.10608203677510608No Hit
CTATAGTAGTCGGCATAGATGCTTTAATTCTAGAATTTGTACTTCTAGTA600.10608203677510608No Hit
GGCATATAATTAGTACAAACACGGTTTAAACACCGTGTAACTATGTTAGT600.10608203677510608No Hit
AGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTG590.10431400282885431No Hit
GAGTAAGACTACTGATGTGCACTTTACATCTGACATTTTAGACTGTACAG590.10431400282885431No Hit
GGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCAT580.10254596888260256No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG580.10254596888260256No Hit
GTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAA570.10077793493635079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCTA100.007073308145.05
TTAACCC100.007073308145.03
CTGCAGA100.007073308145.0145
GAGCTGA451.07302185E-896.6666641
ACACCCC702.2950371E-762.1428575
ACCCCTC702.2950371E-762.1428577
CACCCCT753.6976962E-758.0000046
CCCCTCT753.6976962E-758.0000048
GTATACA858.774732E-751.176471
ATGTTAC858.774732E-751.176477
ATACACC858.774732E-751.176473
ACATATA752.5199213E-548.3333367
ATATAAC752.5199213E-548.3333369
ATCTATT450.00959253648.3333329
TACACCA450.00959253648.333332145
CTGATGT951.8887131E-645.7894744
CACCGTG1801.8189894E-1244.3055578
ACACCGT1801.8189894E-1244.3055577
AACACCG1851.8189894E-1243.108116
GAAGAAT700.001048199141.428578
Produced by FastQC (version 0.11.5)