FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153280
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT19511.2728340292275575No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAA11750.766570981210856No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT8880.5793319415448851No Hit
ATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCT8440.5506263048016702No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT7500.48930062630480164No Hit
GTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTG7270.4742954070981211No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG6780.4423277661795407No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC6740.43971816283924847No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC5300.3457724425887265No Hit
GGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCA5180.33794363256784965No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA5020.3275052192066806No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG5020.3275052192066806No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT4950.32293841336116913No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT4760.3105427974947808No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC4740.3092379958246347No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA4740.3092379958246347No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC4730.3085855949895616No Hit
GTATAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCC4640.302713987473904No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT4460.29097077244258873No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT4410.28770876826722336No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA4280.2792275574112735No Hit
CTCTTGGTAGTGATAAAGCTCACAAGTAGCGAGTGTTATCAGTGCCAAGA4270.2785751565762004No Hit
GTTGTGATGATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTG4190.27335594989561585No Hit
GCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCT4030.2629175365344467No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG3940.25704592901878914No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT3900.2544363256784969No Hit
GTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTC3830.2498695198329854No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC3830.2498695198329854No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA3730.2433455114822547No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC3670.23943110647181629No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT3570.2329070981210856No Hit
GTTTAGGTGAAACTGATCTGGCACGTAACTGATAGACGTGTTTTACGCCG3430.22377348643006262No Hit
CTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGC3320.21659707724425886No Hit
CTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATT3240.21137787056367432No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA3120.2035490605427975No Hit
CTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTG3050.19898225469728603No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG3030.19767745302713985No Hit
GTGTTTAATTTATAGTTGCCAATCCTGTAGCGACTGTATGCAGCAAAACC2990.1950678496868476No Hit
CTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA2970.19376304801670144No Hit
GCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAAT2960.19311064718162838No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG2940.19180584551148225No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT2880.18789144050104384No Hit
CTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTT2750.17941022964509395No Hit
CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCT2730.17810542797494783No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT2710.17680062630480167No Hit
CTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAAC2700.1761482254697286No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA2690.17549582463465555No Hit
GGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAA2650.17288622129436326No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA2610.17027661795407098No Hit
ATTTAGAACCAGCCTCATCCACGCACAATTCAATTAAAGGTGCTGATTTT2580.16831941544885176No Hit
CTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACT2570.1676670146137787No Hit
TTCCAGAAGAGCAAGGTTCTTTTAAAAGTACTGTTGTACCTCTAACACAC2560.16701461377870563No Hit
CGTCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGA2540.1657098121085595No Hit
GTATAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC2530.16505741127348644No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG2520.16440501043841338No Hit
GTTCAAGTGAGAACCAAAAGATAATAAGCATAATTAAAACAAGGAATAGC2510.1637526096033403No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT2510.1637526096033403No Hit
GTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTC2500.16310020876826722No Hit
CTTTAAAAGTCCTCATAATAATTAGTAATATCTCTGCTATAGTAACCTGA2490.16244780793319416No Hit
CCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTA2410.1572286012526096No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA2400.15657620041753653No Hit
CTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG2390.15592379958246347No Hit
GTCCTAGATGGTGTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCT2380.1552713987473904No Hit
GTTGTACCTCTAACACACTCTTGGTAGTGATAAAGCTCACAAGTAGCGAG2380.1552713987473904No Hit
GAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAA2370.15461899791231734No Hit
GGTTTATGATGTAATCAAGATTCCAAATGGAAACTTTAAAAGTCCTCATA2320.151356993736952No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG2320.151356993736952No Hit
GTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGTTC2310.15070459290187893No Hit
GTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTCAATTA2280.1487473903966597No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA2260.14744258872651356No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC2260.14744258872651356No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC2240.14613778705636743No Hit
ACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTG2230.14548538622129437No Hit
ACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCT2220.14483298538622127No Hit
CTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATT2140.13961377870563674No Hit
ATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTAC2140.13961377870563674No Hit
GTCTTCATAGAACGAACAACGCACTACAAGACTACCCAATTTAGGTTCCT2120.1383089770354906No Hit
CTCTAACACACTCTTGGTAGTGATAAAGCTCACAAGTAGCGAGTGTTATC2120.1383089770354906No Hit
CATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCA2060.1343945720250522No Hit
GTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTC2010.13113256784968685No Hit
CTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTG1990.12982776617954073No Hit
GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGC1980.12917536534446766No Hit
TTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATC1970.12852296450939457No Hit
CTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCC1970.12852296450939457No Hit
TCCACGCACAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAAT1960.1278705636743215No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTCACCTTTTAC1960.1278705636743215No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT1930.1259133611691023No Hit
CATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTA1910.12460855949895616No Hit
GGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACAT1900.1239561586638831No Hit
GTGTTATCAGTGCCAAGAAAAGAATAATTTTCATGTTCGTTTAATCAATC1900.1239561586638831No Hit
ATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAG1900.1239561586638831No Hit
TAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAG1890.12330375782881No Hit
AGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGG1880.12265135699373694No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT1870.12199895615866388No Hit
GACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAAC1860.12134655532359082No Hit
GAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTAC1860.12134655532359082No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG1850.12069415448851774No Hit
AAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCT1850.12069415448851774No Hit
GATGAAATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCAT1850.12069415448851774No Hit
TTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAG1840.12004175365344467No Hit
GTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCA1830.11938935281837161No Hit
ATTATACAGTTTCCTGTTCACCTTTTACAATTAATTGCCAGGAACCTAAA1820.11873695198329855No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC1820.11873695198329855No Hit
GTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGG1810.11808455114822548No Hit
GATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCT1760.11482254697286012No Hit
GTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGT1760.11482254697286012No Hit
GGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCG1750.11417014613778706No Hit
GCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTC1740.113517745302714No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG1720.11221294363256784No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC1710.11156054279749478No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC1700.11090814196242171No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC1700.11090814196242171No Hit
GAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGGGGC1680.10960334029227556No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC1680.10960334029227556No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC1680.10960334029227556No Hit
GCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAAT1680.10960334029227556No Hit
TCGATGTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTC1640.10699373695198329No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC1630.10634133611691023No Hit
CCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTC1620.10568893528183716No Hit
ATCCTGTAGCGACTGTATGCAGCAAAACCTGAGTCACCTGCTACACGCTG1610.1050365344467641No Hit
GAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTG1580.10307933194154488No Hit
GTACATCGATATCGGTAATTATACAGTTTCCTGTTCACCTTTTACAATTA1570.10242693110647182No Hit
GTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCA1560.10177453027139875No Hit
CTCCTACTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCA1540.10046972860125261No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT1540.10046972860125261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA600.0145.01
TCGCGTA151.2279896E-4145.05
ATCCCAG100.007085047144.999983
TCCCAAC100.007085047144.999982
TTCCGAC100.007085047144.999982
GTATTAA100.007085047144.999981
TATCCCA100.007085047144.999982
TGCTATC100.007085047144.999982
ATTCCGA100.007085047144.999981
TCCGACC100.007085047144.999983
CCGACCA100.007085047144.999984
CGACCAG100.007085047144.999985
TTAAACC100.007085047144.999984
TGGCCTG100.007085047144.999985
TTTCGCG202.1247943E-6144.999983
CTATCCC100.007085047144.999981
TTTTCGC256.4472824E-6116.02
TATAACC800.0108.749992
GTTTTCG203.8597456E-4108.749991
CTGAACA203.8597456E-4108.749994
Produced by FastQC (version 0.11.5)