FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178877
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATT22121.2366039233663355No Hit
TTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTC17901.0006876233389423No Hit
GTTTAATTGTGTACAAAAACTGCCATATTGCAACAAAAGATTGCTGCATT8680.485249640814638No Hit
CAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACAC8060.450588952185021No Hit
GAGTCGTCTTCATCAAATTTGCAGCAGGATCCACAAGAACAACAGCCCTT7910.4422033017101136No Hit
GTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGA7870.43996712825013834No Hit
GCATAATTGTCACCATTACTATGGCAATCAAGCCAGCTATAAAACCTAGC7770.4343766946002001No Hit
GTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCC7660.42822721758526805No Hit
GGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGA7130.39859791924059546No Hit
ATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGC7100.396920789145614No Hit
GTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT6580.3678505341659353No Hit
GCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAA5970.3337488889013121No Hit
GGTAATATTTGTGAAAAATTAAAACCACCAAAATCTTTAATTGGTGGTGT5920.33095367207634296No Hit
CTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATT5730.3203318481414603No Hit
GAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATAC5440.3041195905566395No Hit
TCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAA5400.3018834170966642No Hit
CTATAAAACCTAGCCAAATGTACCATGGCCATTTTATATACTGCTCATAC5280.2951748967167383No Hit
GGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACG5250.29349776662175686No Hit
GTTACCAGACACAAATGTGTTGTCTGTAGTAATGATTTGTGGTTCATAAA5080.28399402941686186No Hit
CTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTC4920.2750493355769607No Hit
ATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATA4800.26834081519703484No Hit
GTTTTGATGGATCTGGTAATATTTGTGAAAAATTAAAACCACCAAAATCT4500.2515695142472202No Hit
AGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTT4340.242624820407319No Hit
GGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGAC4320.24150673367733136No Hit
ATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAA4310.24094769031233754No Hit
TAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAG4280.23927056021735607No Hit
TTACTATGGCAATCAAGCCAGCTATAAAACCTAGCCAAATGTACCATGGC4280.23927056021735607No Hit
ATCTGAAGGAGTAGCATCCTTGATTTCACCTTGCTTCAAAGTTACAGTTC4200.23479821329740547No Hit
GTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAA4200.23479821329740547No Hit
GCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTAT4180.23368012656741782No Hit
GTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGG4050.2264125628224981No Hit
ACCTATCAATTTGCACTTCAGCCTCAACTTTGTCAAGACGTGAAAGGATA4020.22473543272751667No Hit
GCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAA3950.22082212917255992No Hit
ACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCT3930.21970404244257227No Hit
TGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATA3930.21970404244257227No Hit
GTTTACAACTGAAGCATTAATGCCAGAGATGTCACCTAAATCAACATCTG3890.217467868982597No Hit
GTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGA3880.2169088256176032No Hit
CCTTTGAGCACTGGCTCAGAGTCGTCTTCATCAAATTTGCAGCAGGATCC3850.21523169552262167No Hit
GAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAA3810.21299552206264638No Hit
GTCTAATTCAGGTTGCAAAGGATCATAAACTGTGTTGTTGACAATTCCTA3770.21075934860267112No Hit
GTGCAGAAGTGTATTGAGCAATCATTTCATCTGTGAGCAAAGGTGGCAAA3730.20852317514269583No Hit
CCATTACTATGGCAATCAAGCCAGCTATAAAACCTAGCCAAATGTACCAT3720.207964131777702No Hit
GCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTC3720.207964131777702No Hit
ATTTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGAT3680.2057279583177267No Hit
CATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGAT3630.20293274149275758No Hit
CCATATTGTTTGATGAAGCCAGCATCTGCAAGTGTCACTTTGTTGAAAAG3620.20237369812776376No Hit
GTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTC3610.20181465476276994No Hit
GGACATAAGATGATAGCCCTTTCCACAAAAATCAACTCTTTTTGATTGTC3490.19510613438284408No Hit
TTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAAT3490.19510613438284408No Hit
CTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACA3420.19119283082788732No Hit
CTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCA3380.18895665736791203No Hit
CTACAGCAACTGGTCATACAGCAAAGCATAATTGTCACCATTACTATGGC3240.1811300502579985No Hit
CTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCA3220.18001196352801085No Hit
GACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTG3200.17889387679802324No Hit
AGTACACACTCTGACATTTTAGTAGCAGCAAGATTAGCAGAAGCTCTGAT3180.1777757900680356No Hit
GCTATAAAACCTAGCCAAATGTACCATGGCCATTTTATATACTGCTCATA3160.17665770333804792No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA3140.17553961660806028No Hit
GCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTCATTTAAATTC3070.17162631305310352No Hit
AGACTACACCATGAGGTGCTGACTGAGGGAAGGACATAAGATGATAGCCC3050.17050822632311588No Hit
TTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACT3020.16883109622813441No Hit
ACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAAC3010.1682720528631406No Hit
CTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTCATTTAAAT2980.16659492276815913No Hit
CTCCAATACCATTAAACCTATAAGCCATTTGCATAGCAAATGGTATTTGT2970.1660358794031653No Hit
TTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATA2870.16044544575322706No Hit
TCACCATATTGTTTGATGAAGCCAGCATCTGCAAGTGTCACTTTGTTGAA2860.15988640238823326No Hit
TTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAAC2840.15876831565824562No Hit
GAGTAGCATCCTTGATTTCACCTTGCTTCAAAGTTACAGTTCCAATTGTG2790.1559730988332765No Hit
GGCTCAGAGTCGTCTTCATCAAATTTGCAGCAGGATCCACAAGAACAACA2750.1537369253733012No Hit
GTCCAAGTACACACTCTGACATTTTAGTAGCAGCAAGATTAGCAGAAGCT2710.15150075191332593No Hit
GGTCATACAGCAAAGCATAATTGTCACCATTACTATGGCAATCAAGCCAG2710.15150075191332593No Hit
GTTTAAAGCTTGTGCATTTTGGTTGACCACATCTTGAAGTTTTCCAAGTG2700.1509417085483321No Hit
GTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATT2660.1487055350883568No Hit
AGTTATCAGACTCAGACTAATACCTTCCCAGGTAACAAACCAACCAACTT2650.14814649172336297No Hit
CTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCA2590.14479223153340004No Hit
CTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGC2590.14479223153340004No Hit
TTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGA2580.14423318816840622No Hit
ACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAAT2570.1436741448034124No Hit
GTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATG2570.1436741448034124No Hit
GAAGTGTATTGAGCAATCATTTCATCTGTGAGCAAAGGTGGCAAAACAGT2550.14255605807342475No Hit
GTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAAT2540.14199701470843093No Hit
ATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTT2530.1414379713434371No Hit
ATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTG2510.14031988461344946No Hit
AACAAAATCTGAAGGAGTAGCATCCTTGATTTCACCTTGCTTCAAAGTTA2510.14031988461344946No Hit
GTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAA2510.14031988461344946No Hit
GGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAA2380.13305232086852978No Hit
GTGATCAACCTATCAATTTGCACTTCAGCCTCAACTTTGTCAAGACGTGA2380.13305232086852978No Hit
CTCTGACATTTTAGTAGCAGCAAGATTAGCAGAAGCTCTGATTTCTGCAG2370.13249327750353596No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT2370.13249327750353596No Hit
GTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTG2350.1313751907735483No Hit
CTTTTTGATTGTCCAAGTACACACTCTGACATTTTAGTAGCAGCAAGATT2330.13025710404356067No Hit
GTATTGAGCAATCATTTCATCTGTGAGCAAAGGTGGCAAAACAGTAAGGC2310.129139017313573No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA2310.129139017313573No Hit
GGATCATAAACTGTGTTGTTGACAATTCCTATTACAACATCACAGTTACC2280.12746188721859153No Hit
CAACTACAGCAACTGGTCATACAGCAAAGCATAATTGTCACCATTACTAT2280.12746188721859153No Hit
GACATAAGATGATAGCCCTTTCCACAAAAATCAACTCTTTTTGATTGTCC2280.12746188721859153No Hit
GATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCAC2240.12522571375861627No Hit
GACTAATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG2230.12466667039362245No Hit
ATAAATGACCTCTTGCTTGGTTTTGATGGATCTGGTAATATTTGTGAAAA2220.1241076270286286No Hit
CCTTGAATGAGTCTAATTCAGGTTGCAAAGGATCATAAACTGTGTTGTTG2210.12354858366363478No Hit
ATCATAAACTGTGTTGTTGACAATTCCTATTACAACATCACAGTTACCAG2200.12298954029864095No Hit
GAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTG2200.12298954029864095No Hit
CTATTAAATTGGTTGGCAATCAATTTTTGGTTCTCATAGAGAACATTCTG2190.12243049693364715No Hit
GTTGAAAAGTAGATCTTCAATAAATGACCTCTTGCTTGGTTTTGATGGAT2180.12187145356865332No Hit
GTAGTAATGATTTGTGGTTCATAAAAATTCCTTTGTGTTACAAACCAGTG2160.12075336683866568No Hit
TTTGAGCACTGGCTCAGAGTCGTCTTCATCAAATTTGCAGCAGGATCCAC2160.12075336683866568No Hit
CTCTAGCAGCAATATCACCAAGGCAATCACCATATTGTTTGATGAAGCCA2150.12019432347367186No Hit
GTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAAC2140.11963528010867804No Hit
CATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGC2130.11907623674368421No Hit
GGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAA2120.11851719337869038No Hit
GGTATATGCGCTAGTTATCAGACTCAGACTAATACCTTCCCAGGTAACAA2070.11572197655372127No Hit
TCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATG2070.11572197655372127No Hit
GTTGTGAAGTTCTTTTCTTGTGCAGGGACATAAGTCACATGCAAGAAGAC2060.11516293318872746No Hit
GACATAAGTCACATGCAAGAAGACTACACCATGAGGTGCTGACTGAGGGA2040.11404484645873982No Hit
ATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTCATT2030.11348580309374597No Hit
CTCATAAACAAATCCATAAGTTCGTTTATGTGTAATGTAATTTGACTCCT2020.11292675972875216No Hit
GAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATG2020.11292675972875216No Hit
GTGTAACTCCAATACCATTAAACCTATAAGCCATTTGCATAGCAAATGGT2000.11180867299876451No Hit
ATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTG2000.11180867299876451No Hit
GCACTGGCTCAGAGTCGTCTTCATCAAATTTGCAGCAGGATCCACAAGAA1990.11124962963377069No Hit
CCATTAAACCTATAAGCCATTTGCATAGCAAATGGTATTTGTAATGCAGC1970.11013154290378305No Hit
GTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATC1950.1090134561737954No Hit
GTTCAACAGCTATTCCAGTTAAAGCACGGTTTAATTGTGTACAAAAACTG1940.1084544128088016No Hit
GTATATGCGCTAGTTATCAGACTCAGACTAATACCTTCCCAGGTAACAAA1930.10789536944380775No Hit
AATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAA1920.10733632607881392No Hit
CAAGAAGACTACACCATGAGGTGCTGACTGAGGGAAGGACATAAGATGAT1910.1067772827138201No Hit
TTGTAATGCAGCACCTGCACCAAAGGTCCAACCAGAAGTGATTGTACCCG1890.10565919598383247No Hit
ACATATGTCTGCAAACTTTGAAGTCTGCCTGTGATCAACCTATCAATTTG1880.10510015261883865No Hit
TCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATA1880.10510015261883865No Hit
GTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCA1880.10510015261883865No Hit
GCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGAC1880.10510015261883865No Hit
AGATTCATTTAAATTCTTGGCAACCTCATTGAGGCGGTCAATTTCTTTTT1860.103982065888851No Hit
ACCATATTGTTTGATGAAGCCAGCATCTGCAAGTGTCACTTTGTTGAAAA1840.10286397915886335No Hit
TCCTTGATTTCACCTTGCTTCAAAGTTACAGTTCCAATTGTGAAGATTCT1830.10230493579386953No Hit
CATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACA1820.1017458924288757No Hit
GATTAGCAGAAGCTCTGATTTCTGCAGCTCTAATTAATTGTTGAGTCACA1790.10006876233389424No Hit
AGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAG1790.10006876233389424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAT1100.0125.227284
GCAGGTA1200.0114.7916643
ATCTGGG203.8608108E-4108.75001145
AAAGCTG451.0864824E-896.6666647
AAGCTGC451.0864824E-896.6666648
GCCAGGT259.3740586E-487.07
GTAGCAA554.37376E-879.090911
ATTCAAT406.6482484E-572.500016
AACTAGA300.001933135372.52
GAAGGAG1200.066.4583365
AGGTATA2200.065.9090965
TGAAGGA1250.063.84
AGGAGTA1250.063.87
AATCTGA1051.6370905E-1162.1428576
TATGCCA350.003561718262.1428574
TGCCAGG350.003561718262.1428576
GGTATAT2450.062.1428571
CTGAAGG1300.061.3461533
CACCCCT502.0057635E-458.06
ACCCCTC502.0057635E-458.07
Produced by FastQC (version 0.11.5)