FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161195
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGACACCTAGTCATGATTGCATCACAACTAGCTACATGTGCATTACC9170.5688762058376501No Hit
GTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATG8580.5322745742733956No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT8370.5192468749030678No Hit
GAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTG7810.48450634324886005No Hit
GTCATATTCTGAGCCCTGTGATGAATCAACAGTTTGAGTTGGTAGTCCCA7170.4448028785011942No Hit
GTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGC6380.39579391420329413No Hit
CTCGTGGACAGCTAGACACCTAGTCATGATTGCATCACAACTAGCTACAT6120.3796643816495549No Hit
ACCATGGACTTGACAATACAGATCATGGTTGCTTTGTAGGTTACCTGTAA5570.3455442166320295No Hit
GTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTTGTAGGTTA4930.30584075188436366No Hit
TGACAATACAGATCATGGTTGCTTTGTAGGTTACCTGTAAAACCCCATTG4740.2940537857874004No Hit
CCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAAT4700.29157231924067123No Hit
ATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAAT4500.27916498650702565No Hit
GTCCTTAGGTATGCCAGGTATGTCAACACATAAACCTTCAGTTTTGAATT4430.2748224200502497No Hit
CTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAG4330.2686187536834269No Hit
CATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTAT4230.2624150873166041No Hit
GTACATAAGTGGTATGAGGTGTTTAAATTGATCTCCAGGCGGTGGTTTAG3990.24752628803622942No Hit
GTCTTATAGCTTCTTCGCGGGTGATAAACATGTTAGGGTAACCATTAACT3960.2456651881261826No Hit
TTACAAGAGTGAGCTGTTTCAGTGGTTTGAGTGAATATGACATAGTCATA3900.2419429883060889No Hit
GTATTATTAGGTGTATCAACATAACCTGTAGGTACAGCAACTAGGTTAAC3880.24070225503272433No Hit
GTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTC3810.23635968857594838No Hit
GTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCAAA3790.23511895530258384No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT3750.23263748875585472No Hit
GTGTAGGATGTAACCCAGTGATTACCTTACTACAATCTTTAAAAAGTCCT3670.2276745556623965No Hit
CTATCAGACATTATGCAAAGTATGCCTACTTTTGCTCTGGTAATAGCAAC3630.22519308911566738No Hit
GCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGA3580.22209125593225595No Hit
GAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTG3520.21836905611216229No Hit
GACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCT3510.21774868947547998No Hit
ATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA3510.21774868947547998No Hit
CTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAAT3340.20720245665188128No Hit
CCATTAACTTGATAATTCATTTTAAAACCCATCATAGAGATGAGTCTTCT3210.19913769037501164No Hit
CTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACA3210.19913769037501164No Hit
GATTATAGACGTAATCAAATCCAATAGAATGATGCCAACAGGCATAAGTG3190.19789695710164706No Hit
GATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATG3160.19603585719160024No Hit
GGTAATAGCAACATTAAATCTGTTTACATTACAAGAGTGAGCTGTTTCAG3130.1941747572815534No Hit
ATCTTTATACGCACTACATTCCAAGGAAGTCCTTTGTACATAAGTGGTAT3030.1879710909147306No Hit
GTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCAT3030.1879710909147306No Hit
GTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAAT3010.18673035764136606No Hit
CATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTA3010.18673035764136606No Hit
GTACACATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCAT2960.18362852445795466No Hit
AGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGAC2880.17866559136449642No Hit
CCTTACTACAATCTTTAAAAAGTCCTGTTACATTTTCAGCTTGTAAAGTT2870.17804522472781414No Hit
ATCATAAACGGATTATAGACGTAATCAAATCCAATAGAATGATGCCAACA2860.17742485809113187No Hit
GTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTC2860.17742485809113187No Hit
ATTTTGTGCTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGC2840.1761841248177673No Hit
GGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGC2790.1730822916343559No Hit
GTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAG2780.17246192499767363No Hit
GTCTTCTATAGGTCATGTCCTTAGGTATGCCAGGTATGTCAACACATAAA2760.17122119172430908No Hit
CCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGAC2740.1699804584509445No Hit
ACACTGAGGTGTGTAGGTGCCTGTGTAGGATGTAACCCAGTGATTACCTT2740.1699804584509445No Hit
ATTCTGAGCCCTGTGATGAATCAACAGTTTGAGTTGGTAGTCCCAAAATC2730.16936009181426223No Hit
GCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATA2730.16936009181426223No Hit
TTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCAC2720.16873972517757993No Hit
ATACAGATCATGGTTGCTTTGTAGGTTACCTGTAAAACCCCATTGTTGAA2720.16873972517757993No Hit
CTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGC2700.1674989919042154No Hit
GGTATGAGGTGTTTAAATTGATCTCCAGGCGGTGGTTTAGCACTAACTCT2690.1668786252675331No Hit
GTCTTTATGTGCTTTAAGCTTATTATCATAAACCAAAGCACTCACAGTGT2640.16377679208412171No Hit
GAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGTGG2620.16253605881075714No Hit
AGTCATGATTGCATCACAACTAGCTACATGTGCATTACCATGGACTTGAC2590.16067495890071032No Hit
ACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACT2550.1581934923539812No Hit
AGCTACATGTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTT2540.15757312571729892No Hit
AGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAG2530.15695275908061665No Hit
GATCATGGTTGCTTTGTAGGTTACCTGTAAAACCCCATTGTTGAACATCA2530.15695275908061665No Hit
CTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAA2520.15633239244393435No Hit
GTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTC2480.15385092589720525No Hit
CACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCAC2470.15323055926052298No Hit
CTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCAT2450.1519898259871584No Hit
CTCACATAGTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTATGC2440.15136945935047613No Hit
GTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGC2440.15136945935047613No Hit
TACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATAT2430.15074909271379386No Hit
CTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCT2400.14888799280374704No Hit
TAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATC2380.14764725953038246No Hit
CACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATAC2360.1464065262570179No Hit
TCTTATAGCTTCTTCGCGGGTGATAAACATGTTAGGGTAACCATTAACTT2340.14516579298365334No Hit
ACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTA2320.14392505971028877No Hit
TCTATAGGTCATGTCCTTAGGTATGCCAGGTATGTCAACACATAAACCTT2310.1433046930736065No Hit
GTTCTAATGTTGAATTCACTTTGAATTTATCAAAACACTCTACACGAGCA2310.1433046930736065No Hit
CCTGTAGGTACAGCAACTAGGTTAACACCTGTAGAAAAACCTAGCTGTAA2300.14268432643692422No Hit
GTACAAAAGACATACTGTTCTAATGTTGAATTCACTTTGAATTTATCAAA2290.14206395980024195No Hit
GCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAA2290.14206395980024195No Hit
GTCATAAAGGTCTCTATCAGACATTATGCAAAGTATGCCTACTTTTGCTC2250.13958249325351282No Hit
GAATATGACATAGTCATATTCTGAGCCCTGTGATGAATCAACAGTTTGAG2250.13958249325351282No Hit
CCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATAC2250.13958249325351282No Hit
TTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCC2240.13896212661683055No Hit
GTGTAGGTGCCTGTGTAGGATGTAACCCAGTGATTACCTTACTACAATCT2210.1371010267067837No Hit
CCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAA2200.13648066007010143No Hit
GTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTATGCGAGCAGAA2200.13648066007010143No Hit
CAGCTAGACACCTAGTCATGATTGCATCACAACTAGCTACATGTGCATTA2200.13648066007010143No Hit
ATTATTAGGTGTATCAACATAACCTGTAGGTACAGCAACTAGGTTAACAC2180.13523992679673688No Hit
GTTCTAGTGTGCCCTTAGTTAGCAATGTGCGTGGTGCAGGTAATTGAGCA2170.1346195601600546No Hit
GTTAATTGCAGATGAAACATCATGCGTGATAACACCCTTATAAAACATTT2140.13275846025000776No Hit
CCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCAT2140.13275846025000776No Hit
GTAAAACCCCATTGTTGAACATCAATCATAAACGGATTATAGACGTAATC2140.13275846025000776No Hit
CAACTAGCTACATGTGCATTACCATGGACTTGACAATACAGATCATGGTT2120.13151772697664318No Hit
GTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTT2120.13151772697664318No Hit
ATCAGACATTATGCAAAGTATGCCTACTTTTGCTCTGGTAATAGCAACAT2120.13151772697664318No Hit
TCATAGAGATGAGTCTTCTATAGGTCATGTCCTTAGGTATGCCAGGTATG2110.1308973603399609No Hit
GTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGC2110.1308973603399609No Hit
CTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGC2070.12841589379323182No Hit
ATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACT2060.12779552715654952No Hit
GTGCAGGTAATTGAGCAGGGTCGCCAATGTACACATAGTGCTTAGCACGT2050.12717516051986724No Hit
TGTACACATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCA2040.12655479388318497No Hit
TCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATG2030.12593442724650267No Hit
CTTTGTAGGTTACCTGTAAAACCCCATTGTTGAACATCAATCATAAACGG2020.1253140606098204No Hit
TTATAGACGTAATCAAATCCAATAGAATGATGCCAACAGGCATAAGTGTC2000.12407332733645583No Hit
TGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCT2000.12407332733645583No Hit
GTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCATAATTTGTGG1990.12345296069977357No Hit
CATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACA1980.1228325940630913No Hit
TTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAG1980.1228325940630913No Hit
CTGTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCA1980.1228325940630913No Hit
CTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGT1960.12159186078972674No Hit
CACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATA1960.12159186078972674No Hit
ATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGA1950.12097149415304445No Hit
GACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCT1930.11973076087967989No Hit
GCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTA1900.11786966096963304No Hit
ATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGT1890.11724929433295078No Hit
GACAACAGGTGCGCTCAGGTCCTATTTTCACAAAATACTTCATAGATGTC1890.11724929433295078No Hit
GGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCAATGCT1890.11724929433295078No Hit
ATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTA1870.11600856105958622No Hit
GCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCAC1860.11538819442290393No Hit
CTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGC1860.11538819442290393No Hit
TTTTAAAACCCATCATAGAGATGAGTCTTCTATAGGTCATGTCCTTAGGT1850.11476782778622166No Hit
GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGA1850.11476782778622166No Hit
GCTTTAAGCTTATTATCATAAACCAAAGCACTCACAGTGTCAACAATTTC1840.11414746114953937No Hit
TTCTCACATAGTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTAT1840.11414746114953937No Hit
CTCTAGTAGCATGACACCCCTCGACATCGAAGCCAATCCATGCACGTACA1840.11414746114953937No Hit
GGTATAATTCTACTACATTTATCTATAGGCAAATATTTTAATGCCTTCTC1840.11414746114953937No Hit
CTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATG1830.1135270945128571No Hit
GTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAAC1820.11290672787617481No Hit
GCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCA1820.11290672787617481No Hit
ATGTTAGGGTAACCATTAACTTGATAATTCATTTTAAAACCCATCATAGA1820.11290672787617481No Hit
CCCTTATAAAACATTTTAAAGCATTGAGCTGATTTGTCTTTATGTGCTTT1810.11228636123949254No Hit
GTTGAACATCAATCATAAACGGATTATAGACGTAATCAAATCCAATAGAA1810.11228636123949254No Hit
GTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGT1810.11228636123949254No Hit
CATTAAATCTGTTTACATTACAAGAGTGAGCTGTTTCAGTGGTTTGAGTG1800.11166599460281025No Hit
GATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGA1800.11166599460281025No Hit
CTCTACACGAGCACGTGCAGGTATAATTCTACTACATTTATCTATAGGCA1790.11104562796612798No Hit
ATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTA1780.11042526132944572No Hit
TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCG1770.10980489469276343No Hit
ATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAAT1750.10856416141939887No Hit
CTATAGGTCATGTCCTTAGGTATGCCAGGTATGTCAACACATAAACCTTC1750.10856416141939887No Hit
GTCTATCACATAGACAACAGGTGCGCTCAGGTCCTATTTTCACAAAATAC1750.10856416141939887No Hit
CCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATG1730.10732342814603431No Hit
CACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCA1720.10670306150935202No Hit
GTGATTACCTTACTACAATCTTTAAAAAGTCCTGTTACATTTTCAGCTTG1710.10608269487266975No Hit
CCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATA1700.10546232823598746No Hit
CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAG1690.10484196159930517No Hit
AATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGA1680.10422159496262293No Hit
AGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGG1680.10422159496262293No Hit
GGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAA1660.10298086168925835No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA1660.10298086168925835No Hit
CTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCT1640.10174012841589379No Hit
ACATTTATCTATAGGCAAATATTTTAATGCCTTCTCACATAGTGCATCAA1630.10111976177921152No Hit
GCTGTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGC1620.10049939514252923No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGG100.007085384144.999983
GTTCCTA100.007085384144.999981
CTAGGTA100.007085384144.999985
TTCCTAG100.007085384144.999982
CCTAGGT100.007085384144.999984
TTTGTGC1150.088.2608643
TTGTGCT1250.081.24
GCACCAA451.2281816E-680.55556145
GATTATT406.646255E-572.499991
GCTAGAC2850.071.228071
CTAGACA2900.070.02
TGTGCTG1450.070.05
TAGACAC2900.070.03
CGTGGAC1050.069.0476153
CTCGTGG1300.066.923071
CACCTAG3050.066.557387
TCGTGGA1350.064.444442
CCTAGTC3300.061.5151569
TGTAGGC606.7798046E-660.4166688
GTAGGCC606.7798046E-660.4166689
Produced by FastQC (version 0.11.5)