FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205971
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG11250.5461933961577116No Hit
GTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACC8360.4058823815003083No Hit
TCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGA7880.3825781299309126No Hit
TTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG7140.3466507420947609No Hit
AATTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT6990.33936816347932475No Hit
ATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATT6940.3369406372741794No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC6680.32431750100742335No Hit
TAATAAAATGATGCAAAGAAGATGTACATTCTAACCATAGCTGAAATCGG6300.3058683018483185No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA6170.29955673371494046No Hit
GTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGC6020.2922741550995043No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG5750.2791655135917192No Hit
CTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGT5610.2723684402173121No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC5470.26557136684290505No Hit
GTAAGAAGACTGGTCAGTAGGATTTATTGGTCTTTTAAACTGTAGTGACA5220.25343373581717815No Hit
CATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTGTCTAAG5210.25294823057614907No Hit
CTGTAACACTATCAACGATGTAAGAAGACTGGTCAGTAGGATTTATTGGT5190.2519772200940909No Hit
CTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATT5190.2519772200940909No Hit
GTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGT5110.2480931781658583No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT5030.2442091362376257No Hit
GTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCA4990.24226711527350936No Hit
GTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAA4970.24129610479145122No Hit
GTATAGAACCAGTACAGTAGGTTGCAATAGTGACATTAGTAGAGTTCAAA4890.23741206286321861No Hit
CTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGC4880.23692655762218956No Hit
GTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAA4860.2359555471401314No Hit
GTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACA4720.22915847376572432No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT4700.22818746328366618No Hit
GGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACAC4580.22236140039131724No Hit
CCTTTAGTGTTATTAGCTCTCAGGTTGTCTAAGTTAACAAAATGAGAGAG4510.21896286370411366No Hit
TTCTAACACCATTAACAATAGTTGTACATTCGACTCTTGTTGCTCTATTA4500.2184773584630846No Hit
CAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTG4370.2121657903297066No Hit
CTATAACATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTG4350.21119477984764845No Hit
GTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTA4340.21070927460661937No Hit
CTTCTATGTCAGATTGATGTGACAATTTAAGACATTCAACAACATCTTTA4250.2063397274373577No Hit
AGATTAATTATTAACCACATAAGCCAAGAATTACTAATAAAATGTACTGC4060.19711512785780522No Hit
CTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTG3950.19177457020648536No Hit
CTAATAAATGTACTACCAGCACAGAATGTATCACAATTAACACAATTCCA3950.19177457020648536No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA3870.18789052827825276No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC3850.18691951779619462No Hit
CAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGAC3840.18643401255516553No Hit
GTACTAATTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTG3810.1849774968320783No Hit
CAATTAACACAATTCCAATTGTGTAGTTTGCAAAAGCCTTTACCTCCATT3730.18109345490384568No Hit
ACCATCAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTT3630.1762384024935549No Hit
ATCAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAG3590.17429638152943863No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT3570.17332537104738044No Hit
CTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGG3520.17089784484223508No Hit
ATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGT3510.170412339601206No Hit
GGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTA3510.170412339601206No Hit
TTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTA3490.16944132911914783No Hit
ATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAAC3480.16895582387811875No Hit
GCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGACATGAC3440.16701380291400247No Hit
GTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATG3380.164100771467828No Hit
GTTATAGGTGAGCATATAGTTATTACAACTATCGCCAGTAACTTCTATGT3370.16361526622679892No Hit
ATTTAAGACATTCAACAACATCTTTAGTTTCTACATCTGAATCAACAAAC3360.16312976098576984No Hit
ATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTG3340.16215875050371167No Hit
GTCTAGTAGTTGCACATGTCAACTTAAAAGGTAAGTTATTCTTTTTAGCA3300.16021672953959537No Hit
GGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTT3280.15924571905753723No Hit
ATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATC3220.15633268761136276No Hit
GCTTTATCAAAGTAAAGATGGATGGAACCATTCTTCACTGTAACACTATC3190.15487617188827552No Hit
GTTATTAGCTCTCAGGTTGTCTAAGTTAACAAAATGAGAGAGAGAATGTC3130.15196314044210107No Hit
ACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTT3120.151477635201072No Hit
GTAGTGACAAGTCTCTCGCAACTTCATCACTAATAAATGTACTACCAGCA3050.14807909851386847No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC3040.14759359327283936No Hit
TTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTT2970.14419505658563586No Hit
GATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAAC2950.1432240461035777No Hit
CATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGAT2910.1412820251394614No Hit
GTCTACAACATGCACATAACTTTTCCATACATAATAAAATGATGCAAAGA2830.13739798321122876No Hit
AGCCAAGAATTACTAATAAAATGTACTGCAAAATAGCTGAAAAACAATTG2820.1369124779701997No Hit
GTCTAAGTTAACAAAATGAGAGAGAGAATGTCTTTCATAAGTCTTTTGAC2810.1364269727291706No Hit
CTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATC2780.13497045700608337No Hit
ACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTT2760.13399944652402523No Hit
GTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGT2740.13302843604196707No Hit
CTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAG2740.13302843604196707No Hit
CTATTACGTTTGTAACACATCATACAAGTTGATGAATTACAACCGTCTAC2690.13060090983682168No Hit
GTATTAACGTAAGCATCAAACATTTTAACTGCAACTTCCGCACTATCACC2680.13011540459579263No Hit
CCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATT2680.13011540459579263No Hit
GTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATA2650.12865888887270538No Hit
GTACAAGATTAATTATTAACCACATAAGCCAAGAATTACTAATAAAATGT2650.12865888887270538No Hit
TCAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGA2640.1281733836316763No Hit
CTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACA2600.12623136266756No Hit
GCACAGAATGTATCACAATTAACACAATTCCAATTGTGTAGTTTGCAAAA2600.12623136266756No Hit
TATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGC2590.12574585742653094No Hit
GCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGG2580.12526035218550183No Hit
CCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGA2580.12526035218550183No Hit
TTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAAT2570.12477484694447276No Hit
CACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAA2550.12380383646241461No Hit
ATTGCAGCCAATCCAAGTACATAGAAAAACCTAGTGAAAAGAATATATGC2520.12234732073932739No Hit
AATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACA2520.12234732073932739No Hit
ACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTA2510.12186181549829832No Hit
AGATAAGACATTGTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAG2500.12137631025726922No Hit
GAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACACAAGT2500.12137631025726922No Hit
TCATACAAGTTGATGAATTACAACCGTCTACAACATGCACATAACTTTTC2480.12040529977521107No Hit
GTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAAT2430.1179777735700657No Hit
ATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGAC2420.1174922683290366No Hit
TTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTA2380.11555024736492031No Hit
ATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTC2350.11409373164183309No Hit
CTTTTAAACTGTAGTGACAAGTCTCTCGCAACTTCATCACTAATAAATGT2340.113608226400804No Hit
TAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTAC2330.11312272115977491No Hit
GTGTTATTAGCTCTCAGGTTGTCTAAGTTAACAAAATGAGAGAGAGAATG2310.11215171067771676No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA2290.11118070019565862No Hit
ATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTG2280.11069519495462954No Hit
TCTAGTAACAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGC2280.11069519495462954No Hit
ATCACCAACATCAGACACTAATGCCTGATCTAGTAACAGTATAGGTTGAC2260.10972418447257137No Hit
AACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGT2260.10972418447257137No Hit
CCATTAGCATAGACATAAAAGGACCTTCTAACACCATTAACAATAGTTGT2240.10875317399051322No Hit
CCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGT2230.10826766874948415No Hit
CCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTAC2210.107296658267426No Hit
TTTGTATAGTTTCTAAAGAAGGATAGGTGTCTAAAGAATCTAAACCACTA2210.107296658267426No Hit
ATTCTTCACTGTAACACTATCAACGATGTAAGAAGACTGGTCAGTAGGAT2200.10681115302639693No Hit
GTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACT2190.10632564778536784No Hit
TGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTAC2180.10584014254433877No Hit
CTATCAACGATGTAAGAAGACTGGTCAGTAGGATTTATTGGTCTTTTAAA2160.10486913206228062No Hit
ATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAA2160.10486913206228062No Hit
CTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGT2140.10389812158022246No Hit
CGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAA2130.1034126163391934No Hit
ATGTATCACAATTAACACAATTCCAATTGTGTAGTTTGCAAAAGCCTTTA2120.10292711109816431No Hit
GTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCA2100.10195610061610615No Hit
TCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGA2100.10195610061610615No Hit
GAATGTATCACAATTAACACAATTCCAATTGTGTAGTTTGCAAAAGCCTT2080.100985090134048No Hit
ATGTACTACCAGCACAGAATGTATCACAATTAACACAATTCCAATTGTGT2070.10049958489301893No Hit
TTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTGTCTAAGTT2060.10001407965198984No Hit
CTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCT2060.10001407965198984No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGA100.0070868046144.999986
AACCCTA100.0070868046144.999986
CCCTAAT100.0070868046144.999988
ATAATTC502.0064808E-458.0145
GTACTAA2350.055.5319181
AGGCGTG400.006044093554.3749969
ATAGGCG450.00962835548.3333327
TAGGCGT450.00962835548.3333328
ATTATAT2850.045.7894742
CTAATTA2600.041.8269234
GCCTGCT3150.041.4285749
ATATAAC700.001054686141.42857145
TGCCTGC2850.040.7017568
TAGCGGT1252.7049282E-740.69
GTAGCGG1252.7049282E-740.68
CAGTTCT4300.040.4651159
ACAGTTC4350.040.0000048
TTATATG3350.038.9552233
CTAACAA750.001480649938.6666643
ATATAAT1502.5824193E-838.666664145
Produced by FastQC (version 0.11.5)