FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287052
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA31741.1057230048910998No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG18610.6483145910845421No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT15720.5476359683959701No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC14690.5117539679221883No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT13980.48701977342084357No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC13230.4608921031729443No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC11960.41664924821983473No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG11590.40375959756420443No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG11200.39017320903529673No Hit
CCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCT10420.36300043197748144No Hit
GTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTA10170.354291208561515No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT9830.3424466647158006No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC9500.3309504898067249No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA9480.3302537519334476No Hit
ATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAG8960.3121385672282374No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA8080.2814821008040355No Hit
CAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTT7680.26754734333848923No Hit
CATCAGCTCCATCCAAATAAGTTGGACCAAACTGTTGTCCATATGTCATT7430.2588381199225227No Hit
GCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTG7350.2560511684294135No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG7240.25221911012638826No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG7140.24873542076000169No Hit
GTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCA7120.24803868288672437No Hit
CCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTTAAGATTGTCA7040.24525173139361509No Hit
CTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTAC6890.2400261973440352No Hit
CTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTA6780.23619413904101No Hit
ACTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAAC6590.2295751292448755No Hit
ATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTA6520.22713654668840488No Hit
GGTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTAT6500.22643980881512757No Hit
ATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCT6490.2260914398784889No Hit
ATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATA6410.22330448838537967No Hit
CTCTTAAGAAGTTGAATGTCTTCACCTTTGTTAACATTTGGGCCGACAAC6400.22295611944874097No Hit
ATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAG6280.21877569220907708No Hit
TATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGG6270.21842732327243844No Hit
ATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAA6220.21668547858924517No Hit
GTTATAAATGGCTTAACTTCCTCTTTAGGAATCTCAGCGATCTTTTGTTC6170.21494363390605187No Hit
GTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGA6060.21111157560302662No Hit
CTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAAT6060.21111157560302662No Hit
GTGTCTTAAGATTGTCAAAGGTGATAACTTCACCATCTAGGTGGAATGTG6050.21076320666638795No Hit
GTATAGGGTCAGCACCAAAAATACCAGCTGATAATAATGGTGCAAGTAGA5910.20588604155344675No Hit
ATATAAAGTAACAAGTTTTCTGTGAGGAACTTAGTTTCTTCCAGAGTTGT5910.20588604155344675No Hit
CTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCA5840.20344745899697614No Hit
GTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTT5780.20135724537714422No Hit
ATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGAT5620.1957833423909257No Hit
GTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACAT5590.19473823558100972No Hit
GAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAA5470.19055780834134584No Hit
GTTGTAAACACCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTT5460.19020943940470716No Hit
CTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCTGATAATAAT5370.18707411897495924No Hit
ATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCAATCTTTATAG5180.18045510917882476No Hit
CCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAA5040.17557794406588353No Hit
CTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGAC5020.17488120619260622No Hit
GTGTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTG4990.17383609938269026No Hit
CTCTTGTATTTTAATACCCTTATATTTACGCTGTATAGTTGAAACTATGG4940.17209425469949696No Hit
GTGTTACATAGCCAAGTGGCATTGTAACAAGAGTTTCATTTAGATCGTTA4900.1707007789529423No Hit
GTCTTAAGATTGTCAAAGGTGATAACTTCACCATCTAGGTGGAATGTGGT4850.16895893426974903No Hit
CTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAAT4840.1686105653331104No Hit
AATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTC4800.16721708958655573No Hit
CTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCC4710.16408176915680783No Hit
TTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGG4660.16233992447361453No Hit
GACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGT4660.16233992447361453No Hit
GTCTTTGCCTCCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGT4630.16129481766369855No Hit
ATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCA4620.1609464487270599No Hit
ACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTC4540.15815949723395065No Hit
GGTTAATGTTGTCTACTGTTGTAAACACCTTAATAGTCCTCACTTCTCTC4360.1518888563744548No Hit
CCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAG4350.15154048743781615No Hit
GCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACA4290.1494502738179842No Hit
GATAATAATGGTGCAAGTAGAACTTCGTGCTGATTAAAATTTTCATAAGC4240.1477084291347909No Hit
TCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAAATGTTCTTCAGG4180.14561821551495896No Hit
GCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTA4140.14422473976840433No Hit
CTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGGCACTTTGAGA4140.14422473976840433No Hit
GTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTG4130.14387637083176566No Hit
ATATAATTGTCTGTTGGCACTTTTCTCAAAGCTTTCGCTAGCATTTCAGT4120.14352800189512702No Hit
TTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTG4080.1421345261485724No Hit
GTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGG4060.14143778827529507No Hit
ATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAGAG4020.14004431252874042No Hit
GATTACTAGTGTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATA3990.13899920571882446No Hit
GTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAATTGTCTGT3970.13830246784554714No Hit
AATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTGTA3910.1362122542257152No Hit
CATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAG3870.13481877847916057No Hit
CAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAA3850.13412204060588326No Hit
CTCTTGAACAACATCACCCACTATATATGGAGCATCTTTCTTTAAGAAAG3810.13272856485932863No Hit
GTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTAT3810.13272856485932863No Hit
TCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACAT3770.131335089112774No Hit
CAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAG3760.13098672017613533No Hit
CTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAA3720.1295932444295807No Hit
TGTATAGGGTCAGCACCAAAAATACCAGCTGATAATAATGGTGCAAGTAG3620.12610955506319413No Hit
GACATGTACCTACCCAGAAAACTAGGATCAGTTGTGTGGTAGTACTCAAA3620.12610955506319413No Hit
GTTGTAACTTCTTCAACACAAGCTTTGATTTTCTTATCATCTTGTTTTCT3580.1247160793166395No Hit
GGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCAT3580.1247160793166395No Hit
CTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAA3570.12436771038000084No Hit
GTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAG3540.12332260357008486No Hit
TGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTG3530.1229742346334462No Hit
GTTAAAACACCCTCTTGAACAACATCACCCACTATATATGGAGCATCTTT3520.12262586569680754No Hit
GTTATATAATTGTCTGTTGGCACTTTTCTCAAAGCTTTCGCTAGCATTTC3510.12227749676016889No Hit
GTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAAT3480.1212323899502529No Hit
GTTGTGTAGATTGTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGT3460.1205356520769756No Hit
CTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACA3420.11914217633042098No Hit
CTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTT3410.1187938073937823No Hit
GTGGTAGGATTACTAGTGTAATATACACTTTTATCACCTCTCTTAAGAAA3390.118097069520505No Hit
GTTGTTATCTGCCCATTTAATAGAAGTTAAACCATTAACTTGTGGGTATT3370.11740033164722768No Hit
GTCCACAACTTGCGTGTGGAGGTTAATGTTGTCTACTGTTGTAAACACCT3350.11670359377395037No Hit
TAACAATGCAGTGGCAAGATAACAGTTGTTATCTGCCCATTTAATAGAAG3270.1139166422808411No Hit
TTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCCAG3260.11356827334420244No Hit
GTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTATA3260.11356827334420244No Hit
CTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCT3240.11287153547092513No Hit
GGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTA3220.11217479759764783No Hit
GCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTT3210.11182642866100914No Hit
CTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACT3200.11147805972437048No Hit
ATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGGCA3190.11112969078773184No Hit
GACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATG3190.11112969078773184No Hit
GTCCATATGTCATTGACATGTCCACAACTTGCGTGTGGAGGTTAATGTTG3180.11078132185109318No Hit
TCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTA3170.11043295291445453No Hit
AATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAG3170.11043295291445453No Hit
ACATCACCCACTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAAT3160.11008458397781587No Hit
GGATTAAACTTCAACTCTATTTGTTGGAGTGTTAACAATGCAGTGGCAAG3160.11008458397781587No Hit
TGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAA3110.10834273929462258No Hit
GAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAA3100.10799437035798393No Hit
GTTGTGTGGTAGTACTCAAAAGCCTCAACACGTAGAGTGTCATCATTAGG3100.10799437035798393No Hit
ATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGT3090.10764600142134527No Hit
TGTGTAGATTGTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGA3080.10729763248470661No Hit
ACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTG3080.10729763248470661No Hit
GTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACC3070.10694926354806794No Hit
GGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCA3050.10625252567479063No Hit
TGTCAATATAAAGTAACAAGTTTTCTGTGAGGAACTTAGTTTCTTCCAGA2960.10311720524504271No Hit
GCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGA2960.10311720524504271No Hit
ACCTAGTTGTGTAGATTGTCCAGAATAGGACCAATCTTTATAGGAACCAG2940.10242046737176538No Hit
CATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTT2940.10242046737176538No Hit
ATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTAT2920.10172372949848807No Hit
ATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAA2920.10172372949848807No Hit
GTAACAAGTTTTCTGTGAGGAACTTAGTTTCTTCCAGAGTTGTTGTAACT2910.10137536056184943No Hit
AAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAG2910.10137536056184943No Hit
GTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTT2900.10102699162521077No Hit
ACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAAC2880.10033025375193345No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAGC100.0070882486144.999981
AGACGCT100.0070882486144.999989
TCATTCG301.5975074E-596.6666648
CATTCGT301.5975074E-596.6666649
CTCATTC353.4330955E-582.857147
GTCCCCC300.001934331672.56
TGTCCCC300.001934331672.55
TAGAGCA702.8030627E-972.499994
GTAGAGC702.8030627E-972.499993
TCGGCCT350.00356391562.142853145
GAGATGT350.00356391562.1428537
ACTCATT502.0078193E-458.06
ACCTGTT400.006046510354.3749967
AGAGCAG1004.6875357E-850.755
CTGTCCC450.00963219248.3333324
AAATTAG450.00963219248.3333325
GGTGGAT951.9160689E-645.789479
TTGAAGT3050.045.1639374
CTATATA11850.044.6624451
TATATAT11900.044.4747922
Produced by FastQC (version 0.11.5)