FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133524
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC11020.8253197926964442No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC9890.7406908121386417No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT8240.6171175219436207No Hit
GTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTCCT7020.5257481801024535No Hit
GTTTCAAGAGTGCGGGAGAAAATTGATCGTACAACACGAGCAGCCTCTGA6910.5175099607561188No Hit
GGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGA6040.4523531350169258No Hit
GAACAACACCACCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCC5640.42239597375752674No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACT5050.37820916089991313No Hit
CTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTG4450.33327341901081453No Hit
GCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTC4310.3227884125700249No Hit
TCATAGCATCAATGAGTCTCAGTGAATACTGTGAAATTCCATCTAGTATT4030.30181839968844554No Hit
GTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAAC3940.29507803840508073No Hit
CAGTGAATACTGTGAAATTCCATCTAGTATTGTTATAGCGGCCTTCTGTA3910.2928312513106258No Hit
CCAATATTCCAGGCACCTTTTTTAGCTTTTCCTTTTGTAACTTTAAAATT3590.26886552230310656No Hit
TCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATG3580.26811659327162163No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG3530.26437194811419673No Hit
AACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCT3490.26137623198825677No Hit
GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGA3380.25313801264192204No Hit
GTGAAATTCCATCTAGTATTGTTATAGCGGCCTTCTGTAAAACACGCACA3340.25014229651598213No Hit
CAACTGAACAACACCACCTGTAATGTAGGCCATTACAACTAGATTGTTAG3140.23516371588628263No Hit
ATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTGAACAT3110.23291692879182768No Hit
GTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTT2970.222431922351038No Hit
TGCATAAAGAGGACTCAGTATTGATTTCTGTTCACCAATATTCCAGGCAC2950.22093406428806808No Hit
TGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTA2820.21119798687876337No Hit
CAACTAGATTGTTAGTAGCCAAATCAGATGTGAACATCATAGCATCAATG2780.20820227075282347No Hit
GTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACG2730.20445762559539857No Hit
GTGTATGCCCCTCCGTTAAGCTCACGCATGAGTTCACGGGTAACACCACT2640.1977172643120338No Hit
CTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTA2640.1977172643120338No Hit
GTTACAACCTATGTTAGCGCTAGCACGTGGAACCCAATAGGCACACTTGT2620.19621940624906387No Hit
ACATAAGAGAACACACAGCCTCCAAAGGCAATAGTGCGACCACCCTTACG2560.19172583206015398No Hit
GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC2550.190976903028669No Hit
GTCTCAGTGAATACTGTGAAATTCCATCTAGTATTGTTATAGCGGCCTTC2540.19022797399718402No Hit
GCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTT2440.18273868368233426No Hit
GTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAA2390.1789940385249094No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTATGGATTTGTTTATGAGAATCT2360.17674725143045444No Hit
GTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTGAA2360.17674725143045444No Hit
GCAGTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAG2340.17524939336748452No Hit
GTGCCAAGCTCGTCGCCTAAGTCAAATGACTTTAGATCGGCGCCGTAACT2320.17375153530451454No Hit
CTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTT2320.17375153530451454No Hit
ACATAGGGCTGTTCAAGTTGAGGCAAAACGCCTTTTTCAACTTCTACTAA2270.17000689014708964No Hit
ACCATAACATGACCATGAGGTGCAGTTCGAGCATCCGAACGTTTGATGAA2240.16776010305263475No Hit
GGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGG2230.16701117402114976No Hit
ACCTATGTTAGCGCTAGCACGTGGAACCCAATAGGCACACTTGTTATGGC2210.1655133159581798No Hit
GTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATC2200.16476438692669482No Hit
GCATCAATGAGTCTCAGTGAATACTGTGAAATTCCATCTAGTATTGTTAT2190.16401545789520985No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT2160.1617686708007549No Hit
GTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGA2150.16101974176926995No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT2130.1595218837063No Hit
GTATTGTTATAGCGGCCTTCTGTAAAACACGCACAGAATTTTGAGCAGTT2120.15877295467481503No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC2110.15802402564333004No Hit
CATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGT2110.15802402564333004No Hit
GTCTTGATTATGGAATTTAAGGGAAATACAAAATTTGGACATTCCCCATT2100.15727509661184505No Hit
CTTCATGAGAGTTGAAAGGCACATTTGGTTGCATTCATTTGGTGACGCAA2100.15727509661184505No Hit
TTATAATCCAAACCTTTCACAGTTTCCACAAAAGCACTTGTGGAAGCAGA2100.15727509661184505No Hit
TTTCAGAACGTTCCGTGTACCAAGCAATTTCATGCTCATGTTCACGGCAG2090.1565261675803601No Hit
GTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATA2080.15577723854887512No Hit
GGATTCAACAATTTGTTTGAATGCTTTATAATCCAAACCTTTCACAGTTT2080.15577723854887512No Hit
GGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAG2070.15502830951739013No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA2030.15203259339145023No Hit
CCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTC2020.15128366435996526No Hit
GGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACC2010.15053473532848027No Hit
CTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTT2000.14978580629699528No Hit
CTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACG2000.14978580629699528No Hit
CCTTTATTACCGTTCTTACGAAGAAGAACCTTGCGGTAAGCCACTGGTAT1950.1460411611395704No Hit
CACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTT1930.14454330307660046No Hit
AATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTA1860.13930079985620564No Hit
CATGAGGGACAAGGACACCAAGTGTCTCACCACTACGACCGTACTGAATG1850.13855187082472065No Hit
CTGTAAAACACGCACAGAATTTTGAGCAGTTTCAAGAGTGCGGGAGAAAA1840.13780294179323568No Hit
GCTATGTTTAGTGTTCCAGTTTTCTTGAAAATCTTCATAAGGATCAGTGC1840.13780294179323568No Hit
CTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGCATGAAGCTTT1840.13780294179323568No Hit
TTGTTATAGCGGCCTTCTGTAAAACACGCACAGAATTTTGAGCAGTTTCA1830.1370540127617507No Hit
GAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCT1830.1370540127617507No Hit
GTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCA1810.13555615469878074No Hit
AAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGG1810.13555615469878074No Hit
CCTGTATGGTTACAACCTATGTTAGCGCTAGCACGTGGAACCCAATAGGC1810.13555615469878074No Hit
GTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAG1790.13405829663581081No Hit
TCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTG1790.13405829663581081No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA1760.13181150954135587No Hit
ACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGG1740.13031365147838592No Hit
GCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTC1740.13031365147838592No Hit
GGTAACACCACTGCTATGTTTAGTGTTCCAGTTTTCTTGAAAATCTTCAT1740.13031365147838592No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA1740.13031365147838592No Hit
GTCTTTAATGCACTCAAGAGGGTAGCCATCAGGGCCACAGAAGTTGTTAT1720.12881579341541594No Hit
GATGTGAACATCATAGCATCAATGAGTCTCAGTGAATACTGTGAAATTCC1720.12881579341541594No Hit
ACACCACCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATC1720.12881579341541594No Hit
CAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCG1710.12806686438393097No Hit
GCATAAAGAGGACTCAGTATTGATTTCTGTTCACCAATATTCCAGGCACC1710.12806686438393097No Hit
CTGCTATGTTTAGTGTTCCAGTTTTCTTGAAAATCTTCATAAGGATCAGT1700.127317935352446No Hit
CTCAAGAGGGTAGCCATCAGGGCCACAGAAGTTGTTATCGACATAGCGAG1690.12656900632096102No Hit
GTACAACACGAGCAGCCTCTGATGCAAATGCATAAAGAGGACTCAGTATT1670.12507114825799107No Hit
GTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG1660.1243222192265061No Hit
CTTGTAGATCTGTTCTCTAAACGAACATGAAAATTATTCTTTTCTTGGCA1630.12207543213205117No Hit
CAGTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTC1620.12132650310056618No Hit
CCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACT1610.12057757406908122No Hit
ATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTG1590.11907971600611125No Hit
AACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCA1580.11833078697462629No Hit
CCGTTAAGCTCACGCATGAGTTCACGGGTAACACCACTGCTATGTTTAGT1570.11758185794314131No Hit
CGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGG1570.11758185794314131No Hit
ATCCAAACCTTTCACAGTTTCCACAAAAGCACTTGTGGAAGCAGAAAAAG1560.11683292891165632No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG1550.11608399988017135No Hit
CATTAAGACCTTCGGAACCTTCTCCAACAACACCTGTATGGTTACAACCT1550.11608399988017135No Hit
TCCGTGTACCAAGCAATTTCATGCTCATGTTCACGGCAGCAGTATACACC1540.11533507084868638No Hit
CCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGCATGAAGC1540.11533507084868638No Hit
AATCAGATGTGAACATCATAGCATCAATGAGTCTCAGTGAATACTGTGAA1530.11458614181720139No Hit
GCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC1530.11458614181720139No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1520.11383721278571642No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA1520.11383721278571642No Hit
GTTATAGCGGCCTTCTGTAAAACACGCACAGAATTTTGAGCAGTTTCAAG1520.11383721278571642No Hit
GTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCG1520.11383721278571642No Hit
GATAAGACCTCCTCCACGGAGTCTCCAAAGCCACGTACGAGCACGTCGCG1500.11233935472274648No Hit
TGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGAC1500.11233935472274648No Hit
TCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACT1490.11159042569126149No Hit
ATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGT1490.11159042569126149No Hit
CCTTTCACAGTTTCCACAAAAGCACTTGTGGAAGCAGAAAAAGATGCCAA1490.11159042569126149No Hit
ATATTGATGTTGACTTTCTCTTTTTGGAGTATTTCAAGAAGGTTGTCATT1470.11009256762829155No Hit
GGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCG1470.11009256762829155No Hit
GTTTAGTGTTCCAGTTTTCTTGAAAATCTTCATAAGGATCAGTGCCAAGC1470.11009256762829155No Hit
CCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCA1460.10934363859680656No Hit
CCCTTGGAGAGTGCTAGTTGCCATCTCTTTTTGAGGGTTATGATTTTGGA1450.10859470956532159No Hit
GAAGTCAACTGAACAACACCACCTGTAATGTAGGCCATTACAACTAGATT1440.10784578053383662No Hit
CTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGC1440.10784578053383662No Hit
GATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGA1410.10559899343938169No Hit
CATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTT1410.10559899343938169No Hit
ACTATGCTCAGGTCCTACTTCTGAATTGTGACATGCTGGACAATAAATTT1400.1048500644078967No Hit
CCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATC1390.10410113537641173No Hit
GGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCG1380.10335220634492676No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG1380.10335220634492676No Hit
CCTTTTTTAGCTTTTCCTTTTGTAACTTTAAAATTACCACAGGATTCAAC1370.10260327731344179No Hit
ATGTTAGCGCTAGCACGTGGAACCCAATAGGCACACTTGTTATGGCAACC1360.1018543482819568No Hit
GTTTAAAGTCACCAACAATATTGATGTTGACTTTCTCTTTTTGGAGTATT1340.10035649021898686No Hit
ATCTAGTATTGTTATAGCGGCCTTCTGTAAAACACGCACAGAATTTTGAG1340.10035649021898686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGCG100.00708403145.0145
TCGAAAC100.00708403145.04
AGGTTAT100.00708403145.01
AAATTAG151.227638E-4144.999985
GTCAACT406.088303E-790.6254
ACTGAAC1200.090.624993
TTGTAGA3450.084.057972
CTGAACA1300.083.653854
CTTGTAG3500.080.785711
AGATCTG3850.077.2077946
AACTGAA1600.077.031252
ATCTGTT4150.071.62658
TGAACAA1150.069.3478245
TAGATCT4400.067.5568165
TGTAGAT4350.066.6666643
GTAGATC4500.064.444444
TTATACT350.003559739862.1428574
CAACTGA2050.060.1219521
TATAAAT400.006039452354.3752
GATCTGT5900.051.610177
Produced by FastQC (version 0.11.5)