FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-70-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-70-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42777
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGAGTAGCCATTCTTTTATTCCTTTACTTTCTTAATAAACTTGC3400.794819646071487No Hit
GTGCTAATACTGGTGCTGAAAAACATCCTGGAAATCAGTAACTTTGGAAA1730.4044229375599037No Hit
CATGAAGACAGTGTAGCTGAGGAACGGAAATCTGCAGTATGACCTCCACT1440.3366294971596886No Hit
GTGTCATGGAGTGGAGTGGAATGGAATGGAACGGAATGCAGTGGAGTGGA1440.3366294971596886No Hit
CATGAAGACAGTGTATCATTAAGAAATAACCATAAAGTGGGCAACCAGCA1410.32961638263552845No Hit
GTGTAGGAATATGTTCACATAGTCACCCAAATGGAGCATCCTACTCTGTC1380.32260326811136825No Hit
GAGGTGTGGTGTGAAGTGGAATGGATTGGAATGGAATCAGATGTAATGGA1370.3202655632699815No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA1360.3179278584285948No Hit
GTGTATCATTAAGAAATAACCATAAAGTGGGCAACCAGCAGCCCTTGGGG1210.28286228580779393No Hit
ACCATAAAGTGGGCAACCAGCAGCCCTTGGGGCTGCTCTGTCTATGGAGT1190.27818687612502047No Hit
GTGTAGCTGAGGAACGGAAATCTGCAGTATGACCTCCACTACTGGCTGGG1180.27584917128363373No Hit
GAATCAGATGTAATGGAATGTAGAGGAGTGAAGTGGAGTGGAATGGAATG1160.27117376160086026No Hit
ATTCCATTCCATTGCATTCCCGTCCTCTCCATTTCATTAGCTTCCATTCC1000.23377048413867266No Hit
ATCCTTAGACTATGGAATAATCTGTTCTGAATTATGTAAGTTCTTCAGGA990.23143277929728595No Hit
CATTCTATTCTATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTC940.21974425509035228No Hit
GGAATCAGATGTAATGGAATGTAGAGGAGTGAAGTGGAGTGGAATGGAAT900.2103934357248054No Hit
GGTGTTTATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGGATCAGTG850.19870491151787176No Hit
GCATACAGGTGTTTATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGG840.19636720667648502No Hit
ATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGGATCAGTGGTGATAA810.18935409215232485No Hit
GTGTTTATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGGATCAGTGG760.17766556794539123No Hit
CTGTTGGTAGTTTGTATTTCTCTGGGATCAGTGGTGATAATCCCCTTTAT760.17766556794539123No Hit
GGATTGGAATGGAATCAGATGTAATGGAATGTAGAGGAGTGAAGTGGAGT750.1753278631040045No Hit
GGAACATAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAAT740.17299015826261777No Hit
GTGCAGCACCGTGAGGTCCAGGGCTTCGAGTCGGCCACCTTCCTAGGCTA720.1683147485798443No Hit
TCCTTAGACTATGGAATAATCTGTTCTGAATTATGTAAGTTCTTCAGGAC710.1659770437384576No Hit
GAACTGAAGGAGATAGAGACACGAAAAACCCTTCAAAAATCAATGAATCC710.1659770437384576No Hit
CTCTTGGAGTCTGGATCAGGACCCCTCTCCTGTAACAATATGTATTTTTA700.16363933889707086No Hit
GGTCCAGGATGAAGCAGTCGCCATTGTTGAAGCTCTCCCAGGACACAGGT690.16130163405568412No Hit
GTGTTCTGAGTGTGCATTCCGCATACTCTATCCTTAGACTATGGAATAAT660.15428851953152395No Hit
GCCTGTAGCTTTCAAAATATCACCTAAGTGTGCTAATACTGGTGCTGAAA650.15195081469013721No Hit
GTCTAAGGATAGAGTATGCGGAATGCACACTCAGAACACAGACACGTGCC640.1496131098487505No Hit
GTTTAAATCATATATTGGTGACATACTTATCACGAGGACAAGGGGGAAAA620.14493770016597704No Hit
GTGTAGTCAGTGGGTGAAATTTATAATTTTAGAAGTTCCACACATGGAAA620.14493770016597704No Hit
GTTCTGAATTATGTAAGTTCTTCAGGACCATGTCCCATGAGTAAAATGCA610.14259999532459033No Hit
ACTAAGATGAGAGCAGAACTGAAGGAGATAGAGACACGAAAAACCCTTCA610.14259999532459033No Hit
GTGTATGATTCTACTTATATGAAATATCTAGAAAAGGCATATTCATAGAG600.1402622904832036No Hit
GTCCTGAAGAACTTACATAATTCAGAACAGATTATTCCATAGTCTAAGGA580.13558688080043013No Hit
ACTCTATCCTTAGACTATGGAATAATCTGTTCTGAATTATGTAAGTTCTT570.1332491759590434No Hit
TGCTAATACTGGTGCTGAAAAACATCCTGGAAATCAGTAACTTTGGAAAA560.13091147111765666No Hit
GAGCAAAACTCCATCTCAAAAAAACTGGAGAAGCAAGAGTAAACAAATTC560.13091147111765666No Hit
GAGTAAACAAATTCAAAAGCTAGCAGAAGACAAGAAATAACTAAGATGAG550.12857376627626996No Hit
GAGCAGAACTGAAGGAGATAGAGACACGAAAAACCCTTCAAAAATCAATG550.12857376627626996No Hit
GAGTTAATGCTGATAGAATTAAAAAGTATGAGTAAAGCAGAAATACAGCA540.12623606143488322No Hit
CAATGTGTCCAGCCTGTAGCTTTCAAAATATCACCTAAGTGTGCTAATAC540.12623606143488322No Hit
GTATGAGAGTTAATGCTGATAGAATTAAAAAGTATGAGTAAAGCAGAAAT540.12623606143488322No Hit
CCCATTCTATTCTATTCCATTCCATTCCATTCCATTCCATTCCATTCCAT540.12623606143488322No Hit
CTTCAGGACCATGTCCCATGAGTAAAATGCAGTCATGCTTCATGAATCTC530.1238983565934965No Hit
GATGAGAGCAGAACTGAAGGAGATAGAGACACGAAAAACCCTTCAAAAAT530.1238983565934965No Hit
CCTGTAACAATATGTATTTTTAAACCTAATCCATCTTTCCCTAGCATGGG530.1238983565934965No Hit
GTATGCATACAGCATTTTAGATTTCCAAGAATATGTTGGAGCATTCTGAA510.11922294691072305No Hit
CATGAAGACAGTGTTTTGAGGTCCATTTAGGAAAAAAATCTACTAACTAC510.11922294691072305No Hit
CATGAAGACAGTGTAGGAATATGTTCACATAGTCACCCAAATGGAGCATC500.11688524206933633No Hit
GTCCAGGATGAAGCAGTCGCCATTGTTGAAGCTCTCCCAGGACACAGGTA500.11688524206933633No Hit
CAGTGTAGCTGAGGAACGGAAATCTGCAGTATGACCTCCACTACTGGCTG480.11220983238656287No Hit
TTATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGGATCAGTGGTGAT480.11220983238656287No Hit
GTCCAACACCATGTCTGGCCTAGGGTAATTGTTCTTTCTAGTCTTTATTC480.11220983238656287No Hit
GCCCTTGGGGCTGCTCTGTCTATGGAGTAGCCATTCTTTTATTCCTTTAC480.11220983238656287No Hit
CTCTTGGACACTGTGTCTAGATTTGTTCTTGCAGCTCTTCTGAAACACAC470.10987212754517614No Hit
CCATATAGGAGCTCACTTCTTTTAATCCATAAGGCCATTTTCATAGGTTG470.10987212754517614No Hit
CTTTTATTCCTTTACTTTCTTAATAAACTTGCTTTCACTTTGCACTGCGG470.10987212754517614No Hit
CTCCCAGGACACAGGTACCTCGGTGGCACGGACCACACGCCGCCCTTTGA450.1051967178624027No Hit
ACCTAAGTGTGCTAATACTGGTGCTGAAAAACATCCTGGAAATCAGTAAC450.1051967178624027No Hit
CTCTATCCTTAGACTATGGAATAATCTGTTCTGAATTATGTAAGTTCTTC450.1051967178624027No Hit
CCTATAGCCTTTTCTACCTGAAGATACTCTGTGTTTTAAAGCTAAGCTTA440.10285901302101597No Hit
GATTGGGACAGTGCAACTTTAAGTAATGAGTCACTCTTGGACACTGTGTC440.10285901302101597No Hit
CTCCTGTAACAATATGTATTTTTAAACCTAATCCATCTTTCCCTAGCATG430.10052130817962925No Hit
GCTCTGTCTATGGAGTAGCCATTCTTTTATTCCTTTACTTTCTTAATAAA430.10052130817962925No Hit
GCCATATCCCCACACCCATCTTCAAAGTAGTCTTTATTATGTATGACTTG430.10052130817962925No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTCA100.0070673055145.01
TGAAGAC1050.0110.476193
GGCTCAA203.8405144E-4108.751
GTAGTAG203.8405144E-4108.753
GTCATGG301.5819234E-596.6666643
GAGTATA301.5819234E-596.6666641
GTAGGAA301.5819234E-596.6666643
AGTATAC301.5819234E-596.6666642
GTGTCAT301.5819234E-596.6666641
GTGTAGG301.5819234E-596.6666641
GACAGTG1550.093.5483867
GGGTAGT259.3249616E-487.01
TAGTCAG259.3249616E-487.04
GGTAGTA259.3249616E-487.02
AAGACAG1450.085.000015
ACAGTGT1650.074.696978
TGTCATG406.590552E-572.52
AGTCAGT300.001923047572.55
TTGGAAC300.001923047572.52
CAGTGTA1100.072.59
Produced by FastQC (version 0.11.5)