FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-70-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-70-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64288
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCT8471.3175087108013936No Hit
ATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAG5660.8804131408661025No Hit
GCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCCACGAGT4840.7528621204579392No Hit
CCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGG4360.6781981085116974No Hit
CGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTA4280.665754106520657No Hit
GTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCA4020.625311100049776No Hit
CTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGAT3780.587979094076655No Hit
ACTTTCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGG3410.5304255848680937No Hit
GCACTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGAC2930.45576157292185165No Hit
ACTTTCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGG2810.4370955699352912No Hit
GGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCA2720.42309606769537084No Hit
GCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACC2700.41998506719761075No Hit
TAACAAAGCACTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGA2680.41687406669985066No Hit
CTCCTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAAT2660.4137630662020906No Hit
GTAGTGTTCCTTAGGTAGGCGATCTCCTGTCATGGTTAGTGGGTGATCCC2490.38731956197112993No Hit
CCTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTT2440.3795420607267297No Hit
CCCTAATTATGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGA2300.35776505724240915No Hit
ACAAAGCACTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAA2270.35309855649576904No Hit
GTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTC2200.34221005475360877No Hit
CCATTTGACTCCTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAA2200.34221005475360877No Hit
GTTCCTAGGTAGTAGAAATCAATTTCCATTTGACTCCTGGGGGAGTGACA2150.3344325535092086No Hit
CCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGATGG2150.3344325535092086No Hit
GTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCA1900.2955450472872076No Hit
GTCAAATGGAAATTGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAA1810.28154554504728724No Hit
GGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACA1790.27843454454952715No Hit
CCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGG1730.2691015430562469No Hit
AAGCACTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTG1710.2659905425584868No Hit
AGGTAGTAGAAATCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCA1670.25976854156296664No Hit
CCATCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGG1630.25354654056744647No Hit
CCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGATGGT1620.25199104031856645No Hit
GTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAA1620.25199104031856645No Hit
ACTTTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCCACGAG1440.22399203583872573No Hit
GCTTAACCCTAATTATGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGA1420.22088103534096565No Hit
TGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACT1350.20999253359880538No Hit
GTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGATGGTGGTGTCAC1310.2037705326032852No Hit
GTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGAC1290.20065953210552512No Hit
CTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGATGGT1250.19443753111000497No Hit
ATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAA1230.1913265306122449No Hit
GGTATATGCTGCTCAAGCACAAGTCCCTCTGAGAAACATGCCATTCTGTG1220.18977103036336485No Hit
GTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCCA1060.16488302638128421No Hit
GGTATATGCCACCACACTCAGCTAATTTTGGCATTTTTAGTAGAGACAGG1060.16488302638128421No Hit
CCTAGTAACCATAATAGGAGATTGCCTAGTAACCATAACAGGAGATCACC1010.15710552513688403No Hit
TGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAG1010.15710552513688403No Hit
GTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGT1010.15710552513688403No Hit
CCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGATGGTG990.15399452463912394No Hit
CACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAGTGA980.1524390243902439No Hit
CTCGTGGATGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGAC970.15088352414136386No Hit
ACTTTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCATCCAC910.14155052264808363No Hit
GGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACT900.13999502239920358No Hit
CTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAGTTT890.13843952215032354No Hit
TTCCTAGGTAGTAGAAATCAATTTCCATTTGACTCCTGGGGGAGTGACAC870.13532852165256348No Hit
GCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCAATTTCC860.13377302140368344No Hit
ACTCCTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAA860.13377302140368344No Hit
CTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTC850.1322175211548034No Hit
ACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGG840.13066202090592335No Hit
GAAATTGACGCTTAACAAAGCACTCGTGGATGGTGGTGTCACTCCCCCAG830.12910652065704328No Hit
GTAATGGTCATTGTAGTGGTGAAGATGATGGTGGTAGTGGTGATGGTGGT820.12755102040816327No Hit
TGCTTAACCCTAATTATGATGGTGGTGTCACTCCCCCAGGAGTCAAATGG810.12599552015928323No Hit
CAAACCAACCAACTTTCAATTTCCATTTGACTCCTGGGGGAGTGACACCA780.1213290194126431No Hit
GTGTAGGGACCGCCCCTAGTTTTCTGGTAAGTGGCCCAGGTATGCTTAGG770.11977351916376305No Hit
AATTGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTA770.11977351916376305No Hit
CTTTCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGA760.11821801891488302No Hit
TCCCTAGACACAGGAGGCACAGCATGCCACACAGGGCCACATGAGGGAAG750.11666251866600298No Hit
GGGGGAGTGACACCACCATCAATTTCCATTTGACTCCTGGGGGAGTGACA740.11510701841712294No Hit
CTCCCCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCCCCCAGGAG720.11199601791936287No Hit
CACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGG720.11199601791936287No Hit
GGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGC710.11044051767048282No Hit
GTGCAGGTATATGCCACCACACTCAGCTAATTTTGGCATTTTTAGTAGAG700.10888501742160278No Hit
TCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGG690.10732951717272274No Hit
CCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAGTG670.10421851667496267No Hit
AATGGAAATTGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGA670.10421851667496267No Hit
GGGGGAGTGACACCACCATCATCCACGAGTGCTTTGTTAAGCGTCAATTT670.10421851667496267No Hit
TGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACA670.10421851667496267No Hit
GTAGTAGACTGTGAAATGGAAGATGAAGATCTAGGCCAATGGATGAGAAA670.10421851667496267No Hit
GTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTC660.10266301642608262No Hit
AACCATGACAGGAGATCGCCCACTAACTGTGACAGGAGATCTCCTAGTAA650.10110751617720258No Hit
CCACCATCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCC650.10110751617720258No Hit
CGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGATGGTGG650.10110751617720258No Hit
CCTGGGGGAGTGACACCACCATCAATTTCCATTTGACTCCTGGGGGAGTG650.10110751617720258No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT151.2246968E-4145.01
CATTGAA100.0070755375145.02
TCGTCGG151.2246968E-4145.04
GATCTTA151.2246968E-4145.01
TATCGTC151.2246968E-4145.02
GTCGGCA151.2246968E-4145.06
TTCCACG351.873559E-9124.285714
GTTCCTA900.0120.8333361
GCTGCTC256.418579E-6116.08
GGCAGCG203.8494327E-4108.759
ATCGTCG203.8494327E-4108.753
CGTCGGC203.8494327E-4108.755
ATGCTGC352.7321948E-7103.571436
CTAGGTA1050.0103.571435
ATATGCT301.5880385E-596.6666644
TATGCTG301.5880385E-596.6666645
AACCCTA1000.094.255
TTAACCC1050.089.76193
AGGTATA259.346533E-487.05
TCGGCAG259.346533E-487.07
Produced by FastQC (version 0.11.5)